Identification and Characterization of an Aspergillus fumigatus “Supermater” Pair

Sugui, Janyce A.; Losada, Liliana; Wang, Wei; Varga, John J.; Ngamskulrungroj, Popchai; Abu‐Asab, Mones; Chang, Yun C.; O’Gorman, Céline M.; Wickes, Brian L.; Nierman, William C.; Dyer, Paul S.; Kwon‐Chung, Kyung J.

doi:10.1128/mbio.00234-11

Cited by 57 publications

(95 citation statements)

References 28 publications

(55 reference statements)

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“…In general, interspecific crosses produced cleistothecia with viable ascospores in 4 to 8 weeks. The average viability of the hybrid ascospores harvested from 8-weekold crosses was less than 1%, significantly lower than that from conspecific pairings of A. parafelis (4%), A. lentulus (8%), and A. fumigatus (40%) (18).…”

Section: Mating Sexual Crosses Included a Fumigatus A Lentulus Amentioning

confidence: 99%

“…Aspergillus wyomingensis isolates CCF 4417 and CCF 4416 were kindly provided by V. Hubka of Charles University, Prague, Czech Republic. Isolates were cultured at 37°C on Aspergillus minimal medium agar (MMA) (17), malt extract agar (MEA), Czapek's solution agar (CZA), or oatmeal agar (OA) (18).…”

Section: Methodsmentioning

confidence: 99%

“…Mating. Strain crosses were carried out as described elsewhere (18). Briefly, strains of the opposite mating type were inoculated pairwise onto OA plates, which were then sealed with Parafilm and incubated at 30°C.…”

Section: Growthmentioning

confidence: 99%

“…The mating plates were monitored weekly for the formation of cleistothecia and viable ascospores. Ascospore viability assays were carried out as described previously (18). Briefly, ascospores were released from cleistothecia and suspended in water at concentrations adjusted to 1 ϫ 10 3 ascospores/ml by hemocytometer counts.…”

Section: Growthmentioning

confidence: 99%

“…Two strains of mice were also used for virulence studies, BALB/c mice (Division of Cancer Treatment, National Cancer Institute, NIH, USA) immunosuppressed with hydrocortisone acetate (Sigma, St. Louis, MO) and gp91phox-deficient mice (B6.129S6-Cybbtm1Din/J) (The Jackson Laboratory, USA). Hydrocortisone acetate treatment was carried out as described elsewhere (18). BALB/c and gp91phox-deficient mice were inoculated with 30 l of conidial suspension containing 3 ϫ 10 7 and 3 ϫ 10 5 conidia/ml, respectively.…”

Section: Growthmentioning

confidence: 99%

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Genetic Relatedness versus Biological Compatibility between Aspergillus fumigatus and Related Species

et al. 2014

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e Aspergillus section Fumigati contains 12 clinically relevant species. Among these Aspergillus species, A. fumigatus is the most frequent agent of invasive aspergillosis, followed by A. lentulus and A. viridinutans. Genealogical concordance and mating experiments were performed to examine the relationship between phylogenetic distance and mating success in these three heterothallic species. Analyses of 19 isolates from section Fumigati revealed the presence of three previously unrecognized species within the broadly circumscribed species A. viridinutans. A single mating type was found in the new species Aspergillus pseudofelis and Aspergillus pseudoviridinutans, but in Aspergillus parafelis, both mating types were present. Reciprocal interspecific pairings of all species in the study showed that the only successful crosses occurred with the MAT1-2 isolates of both A. parafelis and A. pseudofelis. The MAT1-2 isolate of A. parafelis was fertile when paired with the MAT1-1 isolates of A. fumigatus, A. viridinutans, A. felis, A. pseudoviridinutans, and A. wyomingensis but was not fertile with the MAT1-1 isolate of A. lentulus. The MAT1-2 isolates of A. pseudofelis were fertile when paired with the MAT1-1 isolate of A. felis but not with any of the other species. The general infertility in the interspecies crossings suggests that genetically unrelated species are also biologically incompatible, with the MAT1-2 isolates of A. parafelis and A. pseudofelis being the exception. Our findings underscore the importance of genealogical concordance analysis for species circumscription, as well as for accurate species identification, since misidentification of morphologically similar pathogens with differences in innate drug resistance may be of grave consequences for disease management.

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Section: Mating Sexual Crosses Included a Fumigatus A Lentulus Amentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Growthmentioning

confidence: 99%

Section: Growthmentioning

confidence: 99%

Section: Growthmentioning

confidence: 99%

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Genetic Relatedness versus Biological Compatibility between Aspergillus fumigatus and Related Species

et al. 2014

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Culturing and Mating of Aspergillus fumigatus

Ashton

Dyer

2019

CP Microbiology

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Aspergillus fumigatus is an opportunistic human fungal pathogen, capable of causing invasive aspergillosis in patients with compromised immune systems. The fungus was long considered a purely asexual organism. However, a sexual cycle was reported in 2009, with methods described to induce mating under laboratory conditions. The presence of a sexual cycle now offers a valuable tool for classical genetic analysis of the fungus, such as allowing determination of whether traits of interest are mono‐ or poly‐genic in nature. For example, the sexual cycle is currently being exploited to determine the genetic basis of traits of medical importance such as resistance to azole antifungals and virulence, and to characterize the genes involved. The sexual cycle can also be used to assess the possibility of gene flow between isolates. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. This unit describes protocols for culturing of A. fumigatus and for inducing sexual reproduction between compatible MAT1‐1 and MAT1‐2 isolates of the species. The unit also provides working methods for harvesting sexual structures, isolating single‐spore progeny and confirming whether sexual recombination has occurred. © The Authors. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Induction of the Sexual Cycle in Filamentous Ascomycetes

Houbraken

Dyer

2014

Fungal Biology

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The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specifi c statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. While the advice and information in this book are believed to be true and accurate at the date of publication, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein.

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Identification and Characterization of an Aspergillus fumigatus “Supermater” Pair

Cited by 57 publications

References 28 publications

Genetic Relatedness versus Biological Compatibility between Aspergillus fumigatus and Related Species

Genetic Relatedness versus Biological Compatibility between Aspergillus fumigatus and Related Species

Culturing and Mating of Aspergillus fumigatus

Induction of the Sexual Cycle in Filamentous Ascomycetes

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