Identification and characterization of abamectin resistance in Tetranychus urticae Koch populations from greenhouses in Turkey

Çağatay, Naciye Sena; Menault, Pauline; Riga, Maria; Vontas, John; Ay, Recep

doi:10.1016/j.cropro.2018.05.016

Cited by 44 publications

(40 citation statements)

References 31 publications

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“…However, none of the Turkish strains harbored any of the reported abamectin resistance mutations. Similarly, previous studies did also not detect these mutations in Turkish populations (Ilias et al 2014;Çağatay et al 2018), despite the high resistance levels to abamectin (Çağatay et al 2018). Abamectin resistance mutations were not frequently observed in various strains collected from 27 countries (Ilias et al 2014) and mutations were also not found in 26 strains from Washington, USA (Piraneo et al 2015), wheras it has been reported as more common in seven strains from China (Xu et al 2018).…”

Section: Discussionmentioning

confidence: 74%

“…However, a recent study showed that resistance mutations (individually or a combination of both mutations) to abamectin do not confer high level of resistance on their own (Riga et al 2017). Contribution of resistance mechanisms other than target-site mutations seems also to play major role in abamectin resistance as previously reported (Stumpf and Nauen 2002;Khajehali et al 2011, Riga et al 2014Çağatay et al 2018).…”

Section: Discussionmentioning

confidence: 84%

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Resistance incidence and presence of resistance mutations in populations of Tetranychus urticae from vegetable crops in Turkey

et al. 2019

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Tetranychus urticae Koch is one of the most common and harmful pests in vegetable production areas. Similar to other countries, control of T. urticae is mainly based on acaricides in Turkey. However, T. urticae rapidly develops resistance and failures in chemical control have occurred frequently. The toxicity of various acaricides was investigated in 10 T. urticae populations collected from vegetable crops in Turkey. In addition, populations were screened for the presence of all currently known target-site resistance mutations. It was shown that resistance to bifenthrin was the most widespread, but also half of the populations were resistant to abamectin and hexythiazox. Resistance mutations in the voltage-gated sodium channel (VGSC) and chitin synthase 1 were found in various populations. Moreover, for the first time, F1538I and L1024V VGSC mutations were reported for Turkish populations. Mutations that confer resistance to abamectin, bifenazate and METI-I acaricides such as pyridaben were not detected. These results will contribute to the design of an effective resistance management program in Turkey.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 84%

Resistance incidence and presence of resistance mutations in populations of Tetranychus urticae from vegetable crops in Turkey

et al. 2019

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“…It must be noted, however, that even with this approach, the observed resistance ratios among different strains vs. the control vary from 1.69 to 2.32 at the maximum (Table 2). While such ratios are statistically significant, they only represent a small fraction of observable abamectin resistance in the field [11,17,[59][60][61]. This fact either represents an inherent limitation of the Drosophila model in order to fully recapitulate the field conditions (also relevant in the assessment of target-site resistance [42]) or reflects the synergistic action of multiple molecular mechanisms in resistant pest populations, perhaps involving target-site abamectin resistance [14], in order to generate the resistant phenotype.…”

Section: Discussionmentioning

confidence: 99%

Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the Tetranychus urticae CYP392A16-Based Abamectin Resistance in Drosophila

Riga

Ilias

Vontas

et al. 2020

Insects

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Overexpression of the cytochrome P450 monooxygenase CYP392A16 has been previously associated with abamectin resistance using transcriptional analysis in the two-spotted spider mite Tetranychus urticae, an important pest species worldwide; however, this association has not been functionally validated in vivo despite the demonstrated ability of CYP392A16 to metabolize abamectin in vitro. We expressed CYP392A16 in vivo via a Gal4 transcription activator protein/Upstream Activating Sequence (GAL4/UAS) system in Drosophila melanogaster flies, driving expression with detoxification tissue-specific drivers. We demonstrated that CYP392A16 expression confers statistically significant abamectin resistance in toxicity bioassays in Drosophila only when its homologous redox partner, cytochrome P450 reductase (TuCPR), is co-expressed in transgenic flies. Our study shows that the Drosophila model can be further improved, to facilitate the functional analysis of insecticide resistance mechanisms acting alone or in combination.

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“…hominis [72], and the vegetable leafminer, Liriomyza sativae [73]. In T. urticae and R. microplus, CYP, GST, and esterase enzymes were all shown to be linked to abamectin or ivermectin resistance [28,33,74,75]. Clearly, macrocyclic lactone detoxification can involve different enzymes depending upon the species and in many instances, overexpression of these genes has been linked to resistance to other insecticides as well.…”

Section: Discussionmentioning

confidence: 99%

The ABCB Multidrug Resistance Proteins Do Not Contribute to Ivermectin Detoxification in the Colorado Potato Beetle, Leptinotarsa decemlineata (Say)

Favell

McNeil

Donly

2020

Insects

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The Colorado potato beetle, Leptinotarsa decemlineata (Say), is a significant agricultural pest that has developed resistance to many insecticides that are used to control it. Investigating the mechanisms of insecticide detoxification in this pest is important for ensuring its continued control, since they may be contributors to such resistance. Multidrug resistance (MDR) genes that code for the ABCB transmembrane efflux transporters are one potential source of insecticide detoxification activity that have not been thoroughly examined in L. decemlineata. In this study, we annotated the ABCB genes found in the L. decemlineata genome and then characterized the expression profiles across midgut, nerve, and Malpighian tubule tissues of the three full transporters identified. To investigate if these genes are involved in defense against the macrocyclic lactone insecticide ivermectin in this insect, each gene was silenced using RNA interference or MDR protein activity was inhibited using a chemical inhibitor, verapamil, before challenging the insects with a dose of ivermectin. Survival of the insects did not significantly change due to gene silencing or protein inhibition, suggesting that MDR transporters do not significantly contribute to defense against ivermectin in L. decemlineata.

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Identification and characterization of abamectin resistance in Tetranychus urticae Koch populations from greenhouses in Turkey

Cited by 44 publications

References 31 publications

Resistance incidence and presence of resistance mutations in populations of Tetranychus urticae from vegetable crops in Turkey

Resistance incidence and presence of resistance mutations in populations of Tetranychus urticae from vegetable crops in Turkey

Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the Tetranychus urticae CYP392A16-Based Abamectin Resistance in Drosophila

The ABCB Multidrug Resistance Proteins Do Not Contribute to Ivermectin Detoxification in the Colorado Potato Beetle, Leptinotarsa decemlineata (Say)

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