Identification and characterization of a myeloid differentiation factor 88 (MyD88) cDNA from Zhikong scallop Chlamys farreri

Qiu, Limei; Song, Linsheng; Yu, Yundong; Xu, Wei; Ni, Duojiao; Zhang, Qing‐Chun

doi:10.1016/j.fsi.2007.01.012

Cited by 84 publications

(54 citation statements)

References 58 publications

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“…Both MyD88Ts displayed a constitutive expression in all detected tissues, which is consistent with its homologs in other mollusk, such as C. farreri [30], Ruditapes philippinarum [31]. CgMyD88Ts were expressed at highest level in the gills and hemocytes, demonstrating that their expression profile has diversified after gene duplication.…”

Section: Discussionsupporting

confidence: 79%

Expression and function analysis of two naturally truncated MyD88 variants in the Pacific oyster Crassostrea gigas

Xu¹,

Zhang²,

Li³

et al. 2015

Fish & Shellfish Immunology

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Section: Discussionsupporting

confidence: 79%

Expression and function analysis of two naturally truncated MyD88 variants in the Pacific oyster Crassostrea gigas

Xu¹,

Zhang²,

Li³

et al. 2015

Fish & Shellfish Immunology

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“…There is no report about the function of box3 in signal transduction. However, amino acid point mutations had no affect on the structure and signal transduction function Qiu et al, 2007). Except in TLR3 (Biswas and Tergaonkar, 2007), there is a "BB loop" that is exposed at the surface of the three-dimensional structure of box2 of the TIR domain (Ohnishi et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…The TIR domain includes three typical box motifs, namely box1, box2 and box3 (Qiu et al, 2007). The TIR may trigger a series of signal responses through interactions with other proteins having TIR domains Luke et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Identification, expression pattern and functional characterization of As-MyD88 in bacteria challenge and during different developmental stages of Artemia sinica

Qin

Zhao

Luan

et al. 2015

Developmental & Comparative Immunology

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“…Subsequently, a variety of MyD88 genes have been extensively studied in other species, including human [18], mouse [19], chicken [20], frog [21], scallop [22] and fruit fly [23]. All reported MyD88 genes consist of two conserved domains comprising an amino terminal death domain and a typical TIR domain at the carboxyl terminal region.…”

mentioning

confidence: 99%

Functional genomic studies on an immune- and antiviral-related gene of MyD88 in orange-spotted grouper, Epinephelus coioides

et al. 2012

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Myeloid differentiation factor 88 (MyD88) is a universal adaptor protein involved in Toll-like receptors and in interleukin-1 receptor-induced nuclear factor-kappaB (NF-κB) activation. In this study, a new MyD88 gene (designated as Og-MyD88) was cloned from orange-spotted grouper, Epinephelus coioides, based on the expressed sequence tag (EST) obtained following Roche 454 GS-FLX™ sequencing. The full-length Og-MyD88 cDNA is composed of 1682 bp and encodes a deduced polypeptide of 289 amino acids with 86% homology to MyD88 of Siniperca chuatsi. The deduced amino acid sequence of Og-MyD88 contains a typical death domain at the amino terminus and a conserved Toll/IL-1R (TIR) domain at the carboxyl terminus, as well as three highly conserved motifs (Box1, Box2 and Box3) within the C-terminal TIR domain. In healthy fish, Og-MyD88 was found to be strongly expressed in immune-related tissues, including the spleen, head kidney, kidney, liver, skin and intestine, with lower expression in heart, stomach, brain and muscle. Transcripts of Og-MyD88 were found to be markedly up-regulated in fish spleen after challenge with Singapore grouper iridovirus (SGIV), a highly lethal viral pathogen to grouper fish. Furthermore, the full length Og-MyD88 and its N-terminal death domain were capable of inducing NF-κB activity in HEK-293 cells. Overexpressed Og-MyD88 showed the ability to inhibit replication of SGIV in grouper spleen (GS) cells. These results suggest that Og-MyD88 is involved in the grouper immune response to invasion of viral pathogens and may share similar functions to those observed in higher vertebrates.

show abstract

Identification and characterization of a myeloid differentiation factor 88 (MyD88) cDNA from Zhikong scallop Chlamys farreri

Cited by 84 publications

References 58 publications

Expression and function analysis of two naturally truncated MyD88 variants in the Pacific oyster Crassostrea gigas

Expression and function analysis of two naturally truncated MyD88 variants in the Pacific oyster Crassostrea gigas

Identification, expression pattern and functional characterization of As-MyD88 in bacteria challenge and during different developmental stages of Artemia sinica

Functional genomic studies on an immune- and antiviral-related gene of MyD88 in orange-spotted grouper, Epinephelus coioides

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