Identification and characterization of a very long-chain fatty acid elongase gene in the methylotrophic yeast, Hansenula polymorpha

Prasitchoke, Phatthanon; Kaneko, Yoshinobu; Bamba, Takeshi; Fukusaki, Eiichiro; Kobayashi, Akio; Harashima, Satoshi

doi:10.1016/j.gene.2006.11.013

Cited by 15 publications

(4 citation statements)

References 40 publications

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“…Depending on the strain, the major compound accumulated was C18:1, C18:2 or C16:0. No molecules with a longer chain length were observed, by contrast to what has been reported for Saccharomyces cerevisiae and Hansenula polymorpha [43], [44]. Candida albicans can synthesize and accumulate C18:3 to levels of up to 22.7% of total fatty acids [43], but none of the yeasts of the Yarrowia clade was able to synthesize C18:3.…”

Section: Discussioncontrasting

confidence: 59%

Comparative Physiology of Oleaginous Species from the Yarrowia Clade

et al. 2013

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Yarrowia lipolytica is a genetically tractable yeast species that has become an attractive model for analyses of lipid metabolism, due to its oleaginous nature. We investigated the regulation and evolution of lipid metabolism in non-Saccharomycetaceae yeasts, by carrying out a comparative physiological analysis of eight species recently assigned to the Yarrowia clade: Candida alimentaria, Y. deformans, C. galli, C. hispaniensis, C. hollandica, C. oslonensis, C. phangngensis and Y. yakushimensis. We compared the abilities of type strains of these species to grow on 31 non hydrophobic (sugars and other carbohydrate compounds) and 13 hydrophobic (triglycerides, alkanes and free fatty acids) carbon sources. Limited phenotypic diversity was observed in terms of the range of substrates used and, in the case of short-chain fatty acids, their toxicity. We assessed the oleaginous nature of these species, by evaluating their ability to store and to synthesize lipids. The mean lipid content of cells grown on oleic acid differed considerably between species, ranging from 30% of cell dry weight in C. oslonensis to 67% in C. hispaniensis. Lipid synthesis in cells grown on glucose resulted in the accumulation of C18:1 (n-9) as the major compound in most species, except for C. alimentaria and Y. yakushimensis, which accumulated principally C18:2(n-6), and C. hispaniensis, which accumulated both C16:0 and C18:1(n-9). Thus, all species of the clade were oleaginous, but they presented specific patterns of growth, lipid synthesis and storage, and therefore constitute good models for the comparative analysis of lipid metabolism in this basal yeast clade.

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Section: Discussioncontrasting

confidence: 59%

Comparative Physiology of Oleaginous Species from the Yarrowia Clade

et al. 2013

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“…Fatty acids were extracted as described previously (52). Fatty acid methyl esters were prepared as described previously, with little modification (53). Fatty acids were saponified with 0.4 M KOH in methanol and mixed with 1 ml benzene-petroleum ether (1:1 [vol/vol]), followed immediately by shaking and standing.…”

Section: Methodsmentioning

confidence: 99%

Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae

Fang

Chen

Wang

et al. 2017

Appl Environ Microbiol

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The heavy metal cadmium is widely used and released into the environment, posing a severe threat to crops and humans. Saccharomyces cerevisiae is one of the most commonly used organisms in the investigation of environmental metal toxicity. We investigated cadmium stress and the adaptive mechanisms of yeast by screening a genome-wide essential gene overexpression library. A candidate gene, OLE1, encodes a delta-9 desaturase and was associated with high anti-cadmiumstress activity. The results demonstrated that the expression of OLE1 was positively correlated with cadmium stress tolerance and induction was independent of Mga2p and Spt23p (important regulatory factors for OLE1). Moreover, in response to cadmium stress, cellular levels of monounsaturated fatty acids were increased. The addition of exogenous unsaturated fatty acids simulated overexpression of OLE1, leading to cadmium resistance. Such regulation of OLE1 in the synthesis of unsaturated fatty acids may serve as a positive feedback mechanism to help cells counter the lipid peroxidation and cytoplasmic membrane damage caused by cadmium. IMPORTANCE A S. cerevisiae gene encoding a delta-9 desaturase, OLE1, was associated with high anti-cadmium-stress activity. The data suggest that the regulation of OLE1 in the synthesis of unsaturated fatty acids may serve as a positive feedback mechanism to help yeast cells counter the lipid peroxidation and cytoplasmic membrane damage caused by cadmium. The discovery of OLE1 involvement in membrane stability may indicate a novel defense strategy against cadmium stress.

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“…The mechanism of its action was mostly determined using various mutant strains-in essence, it always includes blocking certain enzymes of fatty acid biosynthesis. For instance HpELO1, a fatty acid elongase gene encoding a 319-amino-acid protein, was identified in the methylotrophic yeast Hansenula polymorpha and it is an ortholog of the S. cerevisiae ELO3 gene that is involved in the elongation of VLCFA [20]. Also other yeast mutants lacking endogenous de novo fatty acid synthesis were used for the purpose [21].…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of Phospholipids with Very Long Chain Fatty Acids in Brewer's Yeast

Řezanka

Kolouchová

Gharwalová

et al. 2017

Lipids

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Yeast lipids and fatty acids (FA) were analyzed in Saccharomyces pastorianus from seven breweries and in the dietary yeast supplement Pangamin. GC-MS identified more than 30 FA, half of which were very-long chain fatty acids (VLCFA) with hydrocarbon chain lengths of ≥22 C atoms. Positional isomers ω-9 and ω-7 were identified in FA with C18-C28 even-numbered alkyl chains. The most abundant ω-7 isomer was cis-vaccenic acid. The structure of monounsaturated FA was proved by dimethyl disulfide adducts (position of double bonds and cis geometric configuration) and by GC-MS of pyridyl carbinol esters. Ultra-high performance liquid chromatography-tandem mass spectrometry with negative electrospray ionization identified the phospholipids phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine, with more than 150 molecular species. Wild-type unmutated brewer's yeast strains conventionally used for the manufacture of food supplements were found to contain VLCFA.

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Identification and characterization of a very long-chain fatty acid elongase gene in the methylotrophic yeast, Hansenula polymorpha

Cited by 15 publications

References 40 publications

Comparative Physiology of Oleaginous Species from the Yarrowia Clade

Comparative Physiology of Oleaginous Species from the Yarrowia Clade

Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae

Identification and Characterization of Phospholipids with Very Long Chain Fatty Acids in Brewer's Yeast

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