Identification and characterization of a secreted M28 aminopeptidase protein in Acanthamoeba

Huang, Jian; Chang, Yao; Shih, Ming Chang; Lin, Wei Chen; Huang, Fu Chin

doi:10.1007/s00436-019-06332-8

Cited by 6 publications

(6 citation statements)

References 27 publications

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“…The molecular functions and characteristics of M28AP protein were studied by using anti-M28AP antibody and M28AP mutant produced by CRISPR/Cas9 system. The results showed that M28AP was involved in the degradation of human complement, such as C3b and iC3b, suggesting it played a vital role in pathology ( Huang et al, 2019 ). The study also found that Acanthamoeba is usually associated with biofilms distributed throughout the environment.…”

Section: Acanthamoeba Related Diseasesmentioning

confidence: 99%

Biological characteristics and pathogenicity of Acanthamoeba

et al. 2023

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Acanthamoeba is an opportunistic protozoa, which exists widely in nature and is mainly distributed in soil and water. Acanthamoeba usually exists in two forms, trophozoites and cysts. The trophozoite stage is one of growth and reproduction while the cyst stage is characterized by cellular quiescence, commonly resulting in human infection, and the lack of effective monotherapy after initial infection leads to chronic disease. Acanthamoeba can infect several human body tissues such as the skin, cornea, conjunctiva, respiratory tract, and reproductive tract, especially when the tissue barriers are damaged. Furthermore, serious infections can cause Acanthamoeba keratitis, granulomatous amoebic encephalitis, skin, and lung infections. With an increasing number of Acanthamoeba infections in recent years, the pathogenicity of Acanthamoeba is becoming more relevant to mainstream clinical care. This review article will describe the etiological characteristics of Acanthamoeba infection in detail from the aspects of biological characteristic, classification, disease, and pathogenic mechanism in order to provide scientific basis for the diagnosis, treatment, and prevention of Acanthamoeba infection.

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Section: Acanthamoeba Related Diseasesmentioning

confidence: 99%

Biological characteristics and pathogenicity of Acanthamoeba

et al. 2023

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“…Furthermore, Acanthamoeba spp. degrade components of the complement system, which will support survival in blood [52]. Acanthamoeba spp.…”

Section: Invasion Of the Brain Occurs Via Lungs And Skinmentioning

confidence: 99%

Three encephalitis-causing amoebae and their distinct interactions with the host

Sarink

Meijs

Denzer

et al. 2022

Trends in Parasitology

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“…[25]. We found a novel protein secreted by Acanthamoeba spp., M28AP, from the extracellular secreted proteomic database as a target of the human innate immune defense system [26]. We purified the recombinant M28AP from CHO cells to characterize the biochemical properties of M28AP ( Figure 2A).…”

Section: Biochemical Characterization Of M28apmentioning

confidence: 99%

“…We previously showed that the M20/M25/M40 superfamily aminopeptidase protein, which is an aminopeptidase found in Acanthamoeba-secreted proteins (Asp), causes cell damage and disruption [24]. We also identified a novel protein that was secreted by Acanthamoeba spp., an M28 aminopeptidase (M28AP), as a target of the human innate immune defense [25,26]. However, little is known regarding the biochemical and functional characterization of M28AP.…”

Section: Introductionmentioning

confidence: 99%

The Biochemical and Functional Characterization of M28 Aminopeptidase Protein Secreted by Acanthamoeba spp. on Host Cell Interaction

2019

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Acanthamoeba are a free-living protozoan whose pathogenic strain can cause severe human diseases, such as granulomatous encephalitis and keratitis. As such, the pathogenic mechanism between humans and Acanthamoeba is still unknown. In our previous study, we identified the secreted Acanthamoeba M28 aminopeptidase (M28AP) and then suggested that M28AP can degrade human C3b and iC3b for inhibiting the destruction of Acanthamoeba spp. with the human immune response. We constructed the produced the recombinant M28AP from a CHO cell, which is a mammalian expression system, to characterize the biochemical properties of Acanthamoeba M28AP. The recombinant M28AP more rapidly hydrolyzed Leu-AMC than Arg-AMC and could be inhibited by EDTA treatment. We show that recombinant M28AP can be delivered into the individual cell line and cause cell line apoptosis in a co-culture model. In conclusion, we successfully investigated the potential molecular characteristics of M28AP.

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Identification and characterization of a secreted M28 aminopeptidase protein in Acanthamoeba

Cited by 6 publications

References 27 publications

Biological characteristics and pathogenicity of Acanthamoeba

Biological characteristics and pathogenicity of Acanthamoeba

Three encephalitis-causing amoebae and their distinct interactions with the host

The Biochemical and Functional Characterization of M28 Aminopeptidase Protein Secreted by Acanthamoeba spp. on Host Cell Interaction

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