Identification and characterization of a fast-neutron-induced mutant with elevated seed protein content in soybean

Prenger, Elizabeth; Ostezan, Alexandra; Mian, Rouf; Stupar, Robert M.; Glenn, Travis C.; Li, Zenglu

doi:10.1007/s00122-019-03399-w

Cited by 11 publications

(9 citation statements)

References 70 publications

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“…Although yield data were not included for the correlation analysis, the previously reported negative correlation between protein and yield and the positive correlation between oil and yield indicated weak correlations among protein, oil, and yield traits due to the low correlation coefficient (r < 0.3) [17,37]. The weak correlation between protein and sucrose was also observed in the M3 population, while protein was repeatedly reported to negatively correlate with sucrose [19,45,46]. In the M3 population, the oil showed no or weak correlation with sucrose, stachyose, and raffinose, meaning the pathways converting carbohydrates to oil were severely affected by the chemical mutagen during the mutagenesis process.…”

Section: Discussionmentioning

confidence: 93%

Assessment of Phenotypic Variations and Correlation among Seed Composition Traits in Mutagenized Soybean Populations

Zhou

Lakhssassi

Cullen

et al. 2019

Genes

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Soybean [Glycine max (L.) Merr.] seed is a valuable source of protein and oil worldwide. Traditionally, the natural variations were heavily used in conventional soybean breeding programs to select desired traits. However, traditional plant breeding is encumbered with low frequencies of spontaneous mutations. In mutation breeding, genetic variations from induced mutations provide abundant sources of alterations in important soybean traits; this facilitated the development of soybean germplasm with modified seed composition traits to meet the different needs of end users. In this study, a total of 2366 ‘Forrest’-derived M2 families were developed for both forward and reverse genetic studies. A subset of 881 M3 families was forward genetically screened to measure the contents of protein, oil, carbohydrates, and fatty acids. A total of 14 mutants were identified to have stable seed composition phenotypes observed in both M3 and M4 generations. Correlation analyses have been conducted among ten seed composition traits and compared to a collection of 103 soybean germplasms. Mainly, ethyl methanesulfonate (EMS) mutagenesis had a strong impact on the seed-composition correlation that was observed among the 103 soybean germplasms, which offers multiple benefits for the soybean farmers and industry to breed for desired multiple seed phenotypes.

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Section: Discussionmentioning

confidence: 93%

Assessment of Phenotypic Variations and Correlation among Seed Composition Traits in Mutagenized Soybean Populations

Zhou

Lakhssassi

Cullen

et al. 2019

Genes

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“…A distinct FN-induced sequence rearrangement at an NAP1 gene model associated with stunted trichrome development in soybean has also been reported . Recently, an FN mutant, where approximately 5.4 Mb (from position 15.7–21.1 Mb) of Chr 12 has been deleted, exhibited higher protein content measured by near infrared (NIR) spectroscopy . This investigation has provided a useful information about the chromosomal deletion and increase in total seed protein content.…”

Section: Introductionmentioning

confidence: 79%

“…13 Recently, an FN mutant, where approximately 5.4 Mb (from position 15.7−21.1 Mb) of Chr 12 has been deleted, exhibited higher protein content measured by near infrared (NIR) spectroscopy. 14 This investigation has provided a useful information about the chromosomal deletion and increase in total seed protein content. However, it is not known how the deleted chromosomal segment affected overall protein synthesis processes using high-throughput quantitative proteomics analyses.…”

Section: ■ Introductionmentioning

confidence: 95%

Proteomic Profiling of Fast Neutron-Induced Soybean Mutant Unveiled Pathways Associated with Increased Seed Protein Content

Islam¹,

Krishnan

Natarajan³

2020

J. Proteome Res.

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Mutagenesis through fast neutron (FN) radiation of soybean resulted in a mutant with a 15% increase in seed protein content. A comparative genomic hybridization analysis confirmed that the mutant is lacking 24 genes located at chromosomes 5 and 10. A tandem mass tag-based proteomic profiling of the wild type and the FN mutant revealed 3,502 proteins, of which 206 proteins exhibited increased abundance and 214 proteins showed decreased abundance. Among the abundant proteins, basic 7S globulin increased fourfold, followed by vacuolar-sorting receptor and protein transporters. The differentially expressed proteins were mapped on the global metabolic pathways. It was observed that there was an enrichment of 29 ribosomal proteins, 16 endoplasmic reticular proteins, and several proteins in export metabolic pathways. The deletion of the sequence-specific DNA binding transcription factor along with 23 other genes may have altered the negative regulation of protein syntheses processes, resulting in an increase in the overall protein content of the mutant seed. This mutant is a valuable resource for researchers to understand the metabolic pathways that may affect an increase in seed protein content (the mass spectrometry data files were submitted to massive.ucsd.edu # MassIVE MSV000084228).

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“…Genome mapping techniques such as array CGH for locating gene deletions and duplications have expanded the use of FN mutagenized populations as a resource for reverse genetic studies. A number of examples now indicate enhanced protein levels through gene combinations in lines with high oil ( [54] and references within) and the use of FN mutagenized populations to identify lines with similar potential [40,41]. In this study, a set of FN lines with high oil, protein, and/or known genetic alterations was selected based on deletions to central carbon metabolic genes responsible for partitioning the maternal nutrient sources into biomass (Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…Fast neutron (FN) mutagenesis-based forward genetic screens have been successful at identifying the genetic basis of valuable phenotypes in soybean [33][34][35][36]. Advances in the use of whole genome sequencing and comparative genome hybridization (CGH) techniques for mapping the deletions in mutant populations [37][38][39] also provide a means to identify novel mutations in genomic studies [40,41] that can have applications in plant breeding programs for improved agronomic traits. Studying the role of altered C and N partitioning as a function of development may provide clues to overcome the inverse correlation between oil and protein and to develop soybean lines with improved seed composition.…”

Section: Introductionmentioning

confidence: 99%

On the Inverse Correlation of Protein and Oil: Examining the Effects of Altered Central Carbon Metabolism on Seed Composition Using Soybean Fast Neutron Mutants

et al. 2019

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Protein and oil levels measured at maturity are inversely correlated across soybean lines; however, carbon is in limited supply during maturation resulting in tradeoffs for the production of other reserves including oligosaccharides. During the late stages of seed development, the allocation of carbon for storage reserves changes. Lipid and protein levels decline while concentrations of indigestible raffinose family oligosaccharides (RFOs) increase, leading to a decreased crop value. Since the maternal source of carbon is diminished during seed maturation stages of development, carbon supplied to RFO synthesis likely comes from an internal, turned-over source and may contribute to the reduction in protein and lipid content in mature seeds. In this study, fast neutron (FN) mutagenized soybean populations with deletions in central carbon metabolic genes were examined for trends in oil, protein, sugar, and RFO accumulation leading to an altered final composition. Two lines with concurrent increases in oil and protein, by combined 10%, were identified. A delayed switch in carbon allocation towards RFO biosynthesis resulted in extended lipid accumulation and without compromising protein. Strategies for future soybean improvement using FN resources are described. of 153-12% of biomass is present as sugars and oligosaccharides [4]. Sugars, such as sucrose, represent a metabolizable energy source in animal feed, but raffinose and stachyose that are members of the raffinose family oligosaccharides (RFOs) are indigestible and undesirable for livestock production [5,6]. RFOs have hypothesized roles in seed desiccation tolerance [7-9], stability of liposomes during dehydration, and seed germination [10][11][12], although efforts in breeding and genetic engineering have demonstrated that reduced levels do not impact seed viability [13][14][15]. The RFO and sucrose levels are the result of central carbon metabolism and could adversely impact the levels of oil and protein, which are known to be inversely correlated in mature seeds [16][17][18][19][20][21].Protein and lipid accumulation over the course of seed development is dependent on the supply of amino acids and sugars from the maternal sources (i.e., organic carbon assimilated in the leaf) as well as the metabolism within the developing seed (i.e., cotyledons) [22][23][24][25]. The levels of intermediates of central carbon (C) and nitrogen (N) metabolism that are precursors for storage reserve production, vary depending on the stage of reproductive development and may indicate temporal changes in metabolism [26][27][28]. During maturation, 10-15% of lipids are degraded [28][29][30], coinciding with RFO accumulation, and occurring at the time when there are little to no exogenous resources supplied by the maternal plant. The expression of genes involved in gluconeogenesis and glyoxylate cycle suggest carbon remobilization from lipid may occur. Along with existing sucrose present late in development, carbon from lipid could contribute to central metabolism and enable RFO pro...

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Identification and characterization of a fast-neutron-induced mutant with elevated seed protein content in soybean

Cited by 11 publications

References 70 publications

Assessment of Phenotypic Variations and Correlation among Seed Composition Traits in Mutagenized Soybean Populations

Assessment of Phenotypic Variations and Correlation among Seed Composition Traits in Mutagenized Soybean Populations

Proteomic Profiling of Fast Neutron-Induced Soybean Mutant Unveiled Pathways Associated with Increased Seed Protein Content

On the Inverse Correlation of Protein and Oil: Examining the Effects of Altered Central Carbon Metabolism on Seed Composition Using Soybean Fast Neutron Mutants

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