Proteomic Profiling of Fast Neutron-Induced Soybean Mutant Unveiled Pathways Associated with Increased Seed Protein Content

Islam, Nazrul; Krishnan, Hari B.; Natarajan, Savithiry

doi:10.1021/acs.jproteome.0c00160

Cited by 16 publications

(16 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The protein content of a soybean mutant induced by fast neutron (FN) irradiation was increased by 15%. Islam et al (2020) compared the difference in seed protein expression between the wild-type and FN-induced mutant and found that the level of basic 7S globulin in the mutant was fourfold higher than that in wild-type soybean seeds. This study provides a valuable germplasm resource for clarifying the molecular mechanism regulating the synthesis and degradation of soybean protein.…”

Section: Proteomicsmentioning

confidence: 99%

Soybean genetic resources contributing to sustainable protein production

Guo

Sun²,

Ren³

et al. 2022

Theor Appl Genet

View full text Add to dashboard Cite

Key message Genetic resources contributes to the sustainable protein production in soybean. Abstract Soybean is an important crop for food, oil, and forage and is the main source of edible vegetable oil and vegetable protein. It plays an important role in maintaining balanced dietary nutrients for human health. The soybean protein content is a quantitative trait mainly controlled by gene additive effects and is usually negatively correlated with agronomic traits such as the oil content and yield. The selection of soybean varieties with high protein content and high yield to secure sustainable protein production is one of the difficulties in soybean breeding. The abundant genetic variation of soybean germplasm resources is the basis for overcoming the obstacles in breeding for soybean varieties with high yield and high protein content. Soybean has been cultivated for more than 5000 years and has spread from China to other parts of the world. The rich genetic resources play an important role in promoting the sustainable production of soybean protein worldwide. In this paper, the origin and spread of soybean and the current status of soybean production are reviewed; the genetic characteristics of soybean protein and the distribution of resources are expounded based on phenotypes; the discovery of soybean seed protein-related genes as well as transcriptomic, metabolomic, and proteomic studies in soybean are elaborated; the creation and utilization of high-protein germplasm resources are introduced; and the prospect of high-protein soybean breeding is described.

show abstract

Section: Proteomicsmentioning

confidence: 99%

Soybean genetic resources contributing to sustainable protein production

Guo

Sun²,

Ren³

et al. 2022

Theor Appl Genet

View full text Add to dashboard Cite

show abstract

“…R-studio was used for data visualization. The procedure used for metabolic mapping of omics data is described in refs , .…”

Section: Methodsmentioning

confidence: 99%

“…Genomic, transcriptomic, and proteomic analyses have been applied to some of the FN soybean mutants to characterize their seed composition. − While some investigations were focused on root nodulation and other attributes of plant growth and response to the environment, , fewer studies have been performed on seed quality using metabolic profiling of soybean. Recently, an integrative transcriptomic and metabolomic approach has been directed toward improvement of seed quality in soybean. , …”

Section: Introductionmentioning

confidence: 99%

Metabolic Profiling of a Fast Neutron Soybean Mutant Reveals an Increased Abundance of Isoflavones

Islam¹,

Krishnan

Slovin

et al. 2023

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

A total of 718 metabolites were identified in leaves and seeds of the soybean (Glycine max (L.) Merr., Fabaceae) fast neutron (FN) mutant 2012CM7F040p05ar154bMN15, which was previously shown to have 21 genes deleted and higher protein content in seeds as compared to wild-type. Among the identified metabolites, 164 were found only in seeds, 89 only in leaves, and 465 in both leaves and seeds. Metabolites that exhibited higher abundance in the mutant leaf than in the wild type include the flavonoids afromosin, biochanin A, dihydrodaidzein, and apigenin. Mutant leaves also exhibited a higher accumulation of glyciteinglucoside, dihydrokaempferol, and pipecolate. The seed-only metabolites that were found in higher abundance in the mutant compared to the wild type included 3-hydroxybenzoate, 3-aminoisobutyrate, coenzyme A, N-acetyl-β-alanine, and 1-methylhistidine. Among several amino acids, the cysteine content increased in the mutant leaf and seed when compared to the wild type. We anticipate that the deletion of acetyl-CoA synthase created a negative feedback effect on carbon dynamics, resulting in increased amounts of cysteine and isoflavone-associated metabolites. Metabolic profiling provided new insight into the cascading effect of gene deletions that helps breeders to produce value-added nutritional seed traits.

show abstract

“…In the recent decade, fast neutron radiation-induced mutagenesis has been increasingly used due to its efficiency in rapidly generating a library of knockout genes [ 19 ]. Its ability to create multiple deletions ranging from 1 bp to 18 Mb has been valuable in delineating several biosynthesis pathways in important crops, such as raffinose family oligosaccharide synthesis [ 20 ] and seed protein synthesis in the soybean ( Glycine max ) [ 21 ]. To date, there are about 3200 radiation-induced plant varieties produced from 210 plant species cultivated in over 70 different countries [ 16 ].…”

Section: Mutation Breeding To Increase Plant Varietiesmentioning

confidence: 99%

Green Revolution to Gene Revolution: Technological Advances in Agriculture to Feed the World

Hamdan

Noor

Abd-Aziz

et al. 2022

Plants

View full text Add to dashboard Cite

Technological applications in agriculture have evolved substantially to increase crop yields and quality to meet global food demand. Conventional techniques, such as seed saving, selective breeding, and mutation breeding (variation breeding), have dramatically increased crop production, especially during the ‘Green Revolution’ in the 1990s. However, newer issues, such as limited arable lands, climate change, and ever-increasing food demand, pose challenges to agricultural production and threaten food security. In the following ‘Gene Revolution’ era, rapid innovations in the biotechnology field provide alternative strategies to further improve crop yield, quality, and resilience towards biotic and abiotic stresses. These innovations include the introduction of DNA recombinant technology and applications of genome editing techniques, such as transcription activator-like effector (TALEN), zinc-finger nucleases (ZFN), and clustered regularly interspaced short palindromic repeats/CRISPR associated (CRISPR/Cas) systems. However, the acceptance and future of these modern tools rely on the regulatory frameworks governing their development and production in various countries. Herein, we examine the evolution of technological applications in agriculture, focusing on the motivations for their introduction, technical challenges, possible benefits and concerns, and regulatory frameworks governing genetically engineered product development and production.

show abstract

Proteomic Profiling of Fast Neutron-Induced Soybean Mutant Unveiled Pathways Associated with Increased Seed Protein Content

Cited by 16 publications

References 45 publications

Soybean genetic resources contributing to sustainable protein production

Soybean genetic resources contributing to sustainable protein production

Metabolic Profiling of a Fast Neutron Soybean Mutant Reveals an Increased Abundance of Isoflavones

Green Revolution to Gene Revolution: Technological Advances in Agriculture to Feed the World

Contact Info

Product

Resources

About