Identification and antibiotic susceptibility testing of Klebsiella species from various clinical specimens in a tertiary care hospital

Abstract

Klebsiella are Gram-negative, shorter and thicker rod shaped bacilli. On Mac-Conkey agar it shows mucoid colonies due to presence of polysaccharide capsule.Total 50 isolates of Klebsiella species collected from different clinical samples were studied biochemically. The different samples were urine 19 (38%), pus 15 (30%), blood 11 (22%), and sputum 5 (10%).Antibiotic susceptibility tests were done by Kirby Bauer disk diffusion method and the ESBL detection by double disc synergy method.Klebsiellapneumoniae were the most common 48 (96%), followed by Klebsiellaoxytoca 2 (4%) in our study. Antibiotic resistance to ampicillin were 100%, percentages to cephalosporins were high: cefozolin and cefuroxime 30 (60%), cefotaxime 27 (54%) and cefoperazone and ceftazidime were 28 (56%). Most of those 21 (42%) were from the infection associated in the hospital. Resistance to Aminoglycosides ranged from 60 to 70%. Resistance to co-trimoxazole and tetracycline were 25 (50%) and 27 (54%) respectively. Resistance to fluoroquinolones were 28 (56%). Resistance to carbapenem (meropenem) were 20 (40%) and to piperacillin sulbactam were 21 (42%).Resistance to third generation cephalosporins were 15 (30%) out of which 9 (60%) showed ESBL production using double disc synergy method