Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis)

Zhu, Ying; Hu, Qiaomu; Xu, Wenteng; Li, Hailong; Guo, Hua; Meng, Liang; Wei, Min; Lü, Sheng; Shao, Changwei; Wang, Na; Yang, Guanpin; Chen, Songlin

doi:10.1371/journal.pone.0176122

Cited by 11 publications

(7 citation statements)

References 54 publications

(74 reference statements)

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“…Similar results appeared in two types of medaka ( O. latipes and O. sakaizumii ), which proved Gsdf affected oestrogen production during sex differentiation 19,20 . Simultaneously, Wnt4a is a key gene in the Wnt4/β-catenin1 pathway that regulates gonad development/differentiation 53,54 . CS - wnt4a was also upregulated after RNAi, although the mechanisms remain elusive.…”

Section: Discussionmentioning

confidence: 99%

The autosomal Gsdf gene plays a role in male gonad development in Chinese tongue sole (Cynoglossus semilaevis)

Zhu

Meng

et al. 2018

Sci Rep

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Gsdf is a key gene for testicular differentiation in teleost. However, little is known about the function of Gsdf in Chinese tongue sole (Cynoglossus semilaevis). In this study, we obtained the full-length Gsdf gene (CS-Gsdf), and functional characterization revealed its potential participation during germ cell differentiation in testes. CS-Gsdf transcription was predominantly detected in gonads, while the levels in testes were significantly higher than those in ovaries. During the different developmental stages in male gonads, the mRNA level was significantly upregulated at 86 dph, and a peak appeared at 120 dph; then, the level decreased at 1 and 2 yph. In situ hybridization revealed that CS-Gsdf mRNA was mainly localized in the Sertoli cells, spermatogonia, and spermatids in mature testes. After CS-Gsdf knockdown in the male testes cell line by RNA interference, a series of sex-related genes was influenced, including several sex differentiation genes, CS-Wnt4a, CS-Cyp19a1a and CS-Star. Based on these data, we speculated that CS-Gsdf may play a positive role in germ differentiation and proliferation via influencing genes related to sex differentiation.

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Section: Discussionmentioning

confidence: 99%

The autosomal Gsdf gene plays a role in male gonad development in Chinese tongue sole (Cynoglossus semilaevis)

Zhu

Meng

et al. 2018

Sci Rep

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“…In C. semilaevis , ovarian differentiation can be observed earlier than the testicular differentiation. Histological observations show that gonadal differentiation begins at ~ 62 days post hatching (dph) [ 24 ]. At this stage, primordial germ cells (PGCs) begin mitosis rapidly to form clusters of oocytes.…”

Section: Discussionmentioning

confidence: 99%

Early response to heat stress in Chinese tongue sole (Cynoglossus semilaevis): performance of different sexes, candidate genes and networks

et al. 2020

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Background Temperature is known to affect living organisms and alter the expression of responsive genes, which affects a series of life processes, such as development, reproduction and metabolism. Several genes and gene families have been involved in high temperature responses, such as heat shock protein (hsp) family, Jumonji family and genes related to cortisol synthesis. Gonad is a vital organ related to the existence of a species. However, the comprehensive understanding of gonadal responses to environmental temperature is limited. Results To explore the effects of environmental temperature on genes and gene networks in gonads, we performed acute heat treatment (48 h) on Chinese tongue sole (Cynoglossus semilaevis). Gonadal transcriptome analysis was conducted on females, pseudomales and males exposed to high (28 °C) and normal (22 °C) temperatures. A total of 1226.24 million clean reads were obtained from 18 libraries. Principal component analysis (PCA) and differentially expressed gene (DEG) analysis revealed different performance of sex responses to heat stress. There were 4565, 790 and 1117 specific genes altered their expression level in females, pseudomales and males, respectively. Of these, genes related to hsp gene family, cortisol synthesis and metabolism and epigenetic regulation were involved in early heat response. Furthermore, a total of 1048 DEGs were shared among females, pesudomales and males, which may represent the inherent difference between high and normal temperatures. Genes, such as eef1akmt3, eef1akmt4, pnmt and hsp family members, were found. Conclusions Our results depicted for the first time the gonadal gene expression under acute high temperature treatment in Chinese tongue sole. The findings may provide a clue for understanding the responses of genes and networks to environmental temperature.

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“…Both wnt4a KO ( Kossack et al, 2019 ) and Wnt antagonist dkk1 overexpression cause testis development ( Sreenivasan et al, 2014 ), confirming a key role of the Wnt / β - catenin pathway in ovarian differentiation in zebrafish. Different chemical inhibitors of the Wnt / β - catenin pathway have been used in other fish species, resulting in downregulation of pro-ovarian genes such as cyp19a1a or foxl2 in tongue sole ( Zhu et al, 2017 ) and carp ( Wu et al, 2019 ) or upregulation of pro-testis gene dmrt1 in tilapia ( Wu et al, 2016 ). This suggests a conservation of the Wnt / β - catenin pathway functions in ovarian differentiation in teleosts, although each actor of the pathway may not play the same part, depending on the species.…”

Section: Genes and Pathways Controlling Granulosa Cell Differentiatio...mentioning

confidence: 99%

Becoming female: Ovarian differentiation from an evolutionary perspective

Nicol

Estermann

Yao

et al. 2022

Front. Cell Dev. Biol.

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Differentiation of the bipotential gonadal primordium into ovaries and testes is a common process among vertebrate species. While vertebrate ovaries eventually share the same functions of producing oocytes and estrogens, ovarian differentiation relies on different morphogenetic, cellular, and molecular cues depending on species. The aim of this review is to highlight the conserved and divergent features of ovarian differentiation through an evolutionary perspective. From teleosts to mammals, each clade or species has a different story to tell. For this purpose, this review focuses on three specific aspects of ovarian differentiation: ovarian morphogenesis, the evolution of the role of estrogens on ovarian differentiation and the molecular pathways involved in granulosa cell determination and maintenance.

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Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis)

Cited by 11 publications

References 54 publications

The autosomal Gsdf gene plays a role in male gonad development in Chinese tongue sole (Cynoglossus semilaevis)

The autosomal Gsdf gene plays a role in male gonad development in Chinese tongue sole (Cynoglossus semilaevis)

Early response to heat stress in Chinese tongue sole (Cynoglossus semilaevis): performance of different sexes, candidate genes and networks

Becoming female: Ovarian differentiation from an evolutionary perspective

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