The autosomal Gsdf gene plays a role in male gonad development in Chinese tongue sole (Cynoglossus semilaevis)

Zhu, Ying; Meng, Liang; Xu, Wenteng; Cui, Zhongkai; Zhang, Nianwei; Guo, Hua; Wang, Na; Shao, Changwei; Chen, Songlin

doi:10.1038/s41598-018-35553-7

Cited by 34 publications

(28 citation statements)

References 60 publications

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“…The expression pattern has obvious gender dimorphism. This result was consistent with those in Oncorhynchus mykiss, Oryzias latipes and Cynoglossus semilaevis [11,12,15]. The distinct gonad expression pattern in fish indicated that gsdf may have a particular impact on gonad during the period of sex differentiation and maintenance male characteristics.…”

Section: Discussionsupporting

confidence: 89%

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Functional Analysis Evolution History of Gonadal Soma-derived Factor (gsdf) in Black Rockfish, Sebastes schlegelii

Wang

2019

AJAAR

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As a teleost- and gonad-specific growth factor, gsdf has been indicated to play an important role in sex differentiation and determination. In this study, the complete open reading frame (ORF) of gsdf was isolated from black rockfish, Sebastes schlegelii. Bioinformatics analysis showed that there is a conserved transforming growth factor-β (TGF-β) domain located on the C-terminus of Gsdf. Multiple sequence alignments revealed that fish gsdf were highly conserved in TGF-β domain which suggested their functional conservatism. Synteny analysis provided evidence for the hypothesis that gsdf was originated from fish-specific genome duplication (FSGD). To further explore its function, the expression pattern was examined based on the RNA-seq data and the result showed that significantly sexually dimorphic expression existing between male and female individuals. These results suggested that gsdf might play an important role in maintenance of male characteristics in Sebastes schlegelii.

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Section: Discussionsupporting

confidence: 89%

“…Moreover, gsdf transcription is activated directly by dmy, which established the autosomal gsdf as the first male sex initiator in Oryzias latipes [14]. And in Cynoglossus semilaevis, the autosomal gsdf gene play a positive role in germ differentiation and proliferation via influencing genes related to sex differentiation [15].…”

Section: Introductionmentioning

confidence: 99%

Functional Analysis Evolution History of Gonadal Soma-derived Factor (gsdf) in Black Rockfish, Sebastes schlegelii

Wang

2019

AJAAR

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“…Likewise, expression of gsdf in the same cell types has been observed in several fish species including rainbow trout (Oncorhynchus mykiss), medaka (Oryzias latipes), zebrafish, Nile tilapia (Oreochromis niloticus), Olive flounder (Paralichthys olivaceus), Spotted scat (Scatophagus argus) and Japanese flounder (Paralichthys olivaceus) [24,[42][43][44][45][46][47][48]. Transcripts of gsdf have also been detected within oocytes in Olive flounder, and within spermatogonia and spermatids in Chinese tongue sole (Cynoglossus semilaevis) [46,49]. Although the function(s) of gsdf in male fish seems strongly associated with germ cells, it may differ between species since this gene has been linked to several processes such as proliferation of primordial germ cells and spermatogonia in rainbow trout, sex determination in Oryzias luzonensis, sex differentiation in Chinese tounge sole, and testis differentiation in medaka and Nile tilapia [24,[49][50][51][52][53].…”

Section: Gsdfmentioning

confidence: 83%

“…Transcripts of gsdf have also been detected within oocytes in Olive flounder, and within spermatogonia and spermatids in Chinese tongue sole (Cynoglossus semilaevis) [46,49]. Although the function(s) of gsdf in male fish seems strongly associated with germ cells, it may differ between species since this gene has been linked to several processes such as proliferation of primordial germ cells and spermatogonia in rainbow trout, sex determination in Oryzias luzonensis, sex differentiation in Chinese tounge sole, and testis differentiation in medaka and Nile tilapia [24,[49][50][51][52][53]. Although we do not know the function of Gsdf in salmon testis, it is likely that this protein has one or more roles as described in other fish species.…”

Section: Gsdfmentioning

confidence: 99%

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

et al. 2020

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Background: Sustainability challenges are currently hampering an increase in salmon production. Using sterile salmon can solve problems with precocious puberty and genetic introgression from farmed escapees to wild populations. Recently sterile salmon was produced by knocking out the germ cell-specific dead end (dnd). Several approaches may be applied to inhibit Dnd function, including gene knockout, knockdown or immunization. Since it is challenging to develop a successful treatment against a gene product already existing in the body, alternative targets are being explored. Germ cells are surrounded by, and dependent on, gonadal somatic cells. Targeting genes essential for the survival of gonadal somatic cells may be good alternative targets for sterility treatments. Our aim was to identify and characterize novel germ cell and gonadal somatic factors in Atlantic salmon. Results: We have for the first time analysed RNA-sequencing data from germ cell-free (GCF)/dnd knockout and wild type (WT) salmon testis and searched for genes preferentially expressed in either germ cells or gonadal somatic cells. To exclude genes with extra-gonadal expression, our dataset was merged with available multi-tissue transcriptome data. We identified 389 gonad specific genes, of which 194 were preferentially expressed within germ cells, and 11 were confined to gonadal somatic cells. Interestingly, 5 of the 11 gonadal somatic transcripts represented genes encoding secreted TGF-β factors; gsdf, inha, nodal and two bmp6-like genes, all representative vaccine targets. Of these, gsdf and inha had the highest transcript levels. Expression of gsdf and inha was further confirmed to be gonad specific, and their spatial expression was restricted to granulosa and Sertoli cells of the ovary and testis, respectively. Finally, we show that inha expression increases with puberty in both ovary and testis tissue, while gsdf expression does not change or decreases during puberty in ovary and testis tissue, respectively. Conclusions: This study contributes with transcriptome data on salmon testis tissue with and without germ cells. We provide a list of novel and known germ cell-and gonad somatic specific transcripts, and show that the expression of two highly active gonadal somatic secreted TGF-β factors, gsdf and inha, are located within granulosa and Sertoli cells.

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“…Gsdf is a growth factor and as mentioned above, a member of the Transforming growth factor beta (TGF-β) superfamily [24]. Gsdf has mainly been detected in teleosts, but recently also in the coelacanth Latimeria menadoensis [41] [47], and within spermatogonia and spermatids in Chinese tongue sole (Cynoglossus semilaevis) [50]. The gsdf gene was first described as an essential player in proliferation of primordial germ cells and spermatogonia in rainbow trout [24].…”

Section: Gsdfmentioning

confidence: 99%

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

Kleppe

Edvardsen

Furmanek

et al. 2019

Preprint

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Background Sustainability challenges are currently hampering an increase in salmon production. Using sterile salmon can solve problems with precocious puberty and genetic introgression from farmed escapees to wild populations. Recently sterile salmon was produced by knocking out the germ cell-specific dead end (dnd). Several approaches may be applied to inhibit Dnd function, including gene knockout, knockdown or immunization. Since it is challenging to develop a successful treatment against a gene product already existing in the body, alternative targets are being explored. Germ cells are surrounded by, and dependent on, gonadal somatic cells. Targeting genes essential for the survival of gonadal somatic cells may be good alternative targets for sterility treatments. Our aim was to identify and characterize novel germ cell and gonadal somatic factors in Atlantic salmon. Results We have for the first time analysed RNA-sequencing data from germ cell-free (GCF)/dnd knockout and wild type (WT) salmon testis and searched for genes preferentially expressed in either germ cells or gonadal somatic cells. To exclude genes with extra-gonadal expression, our dataset was merged with available multi-tissue transcriptome data. We identified 389 gonad specific genes, of which 194 were preferentially expressed within germ cells, and 11 were confined to gonadal somatic cells. Interestingly, 5 of the 11 gonadal somatic transcripts represented genes encoding secreted TGF-β factors; gsdf, inha, nodal and two bmp6-like genes, all representative vaccine targets. Of these, gsdf and inha had the highest transcript levels. Expression of gsdf and inha was further confirmed to be gonad specific, and their spatial expression was restricted to granulosa and Sertoli cells of the ovary and testis, respectively. Finally, we show that inha expression increases with puberty in both ovary and testis tissue, while gsdf expression does not change or decreases during puberty in ovary and testis tissue, respectively. Conclusions This study contributes with transcriptome data on salmon testis tissue with and without germ cells. We provide a list of novel and known germ cell- and gonad somatic specific transcripts, and show that the expression of two highly active gonadal somatic secreted TGF-β factors, gsdf and inha, are located within granulosa and Sertoli cells.

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The autosomal Gsdf gene plays a role in male gonad development in Chinese tongue sole (Cynoglossus semilaevis)

Cited by 34 publications

References 60 publications

Functional Analysis Evolution History of Gonadal Soma-derived Factor (gsdf) in Black Rockfish, Sebastes schlegelii

Functional Analysis Evolution History of Gonadal Soma-derived Factor (gsdf) in Black Rockfish, Sebastes schlegelii

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

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