Identification and analysis of the chicken CD3ε gene

Abstract

The chicken T cell receptor CD3epsilon gene was isolated using a degenerate polymerase chain reaction. The 1883 bp long cDNA encoded a transmembrane protein of 16.9 kDa lacking N-linked glycosylation sites. Comparison of the chicken and mammalian CD3epsilon proteins revealed low homology in the extracellular domain with clusters of similarities located around the N-terminal cysteine residue and proximal to the transmembrane region. The high conservation of the cytoplasmic domain included motifs important for signal transduction. The alignment of all CD3gamma, CD3delta and CD3epsilon proteins allowed the identification of highly conserved residues and motifs. Southern blot analysis indicated the presence of a single copy CD3epsilon gene. The expression of the CD3epsilon transcript was limited to T cells and natural killer cells. A recessive mutation of the CD3epsilon gene in the CB chicken strain enabled the mapping of the epitope recognized by the CT3 monoclonal antibody. This analysis of the first non-mammalian CD3epsilon gene provides novel information about evolutionary conserved structural features and its expression in natural killer cells.

Variability of invariant mouse CD3ε chains detected by anti-CD3 antibodies

“…In fact, a cluster of negatively charged amino acids could be formed in the CD3 4 chains of most species by the proximity of the N-terminal tail, the B to C loop, and the F to G loop of the external Ig domain ( Fig. 9; see, for example [19,26] for complete CD3 4 sequence data). The interaction of this cluster with positively charged amino acid side chains located in TCR constant regions might explain why the extracytoplasmic domain of CD3 4 is involved in the interaction with TCR g chains [27].…”

Effect of infection of turkeys with haemorrhagic enteritis adenovirus isolate on the selected parameters of cellular immunity and the course of colibacillosis

“…The CD4 and CD8 T lymphocytes receptors were marked out with monoclonal antibodies intended for chickens, with a cross-reactivity with turkey lymphocytes, which has been certified in multiple studies (Li et al 1999, 2000, Powell et al 2009). The amino acid sequence in the endoplasmatic domain of the Σ chain of CD3 receptor of the T cells is highly conservative (Göbel andFluri 1997, Kothlow et al 2005) and it is very similar in many animal species and humans, therefore the CD3 + cells could be detected in turkeys with the use of Rat Anti Human CD3-APC (clone CD3-12) monoclonal antibodies. The IgM + B cells were marked out with the Goat Anti Chicken IgM-FITC polyclonal antibodies which show cross-reactivity to the turkeys' IgM (van Nerom et al 1997).…”

Characterization of the Region Involved in CD3 Pairwise Interactions within the T Cell Receptor Complex

“…Along this line, we cannot exclude that the major effect of deletions 1 and 14 in CD3-⑀ is to alter the epitope recognized by the conformation-dependent antibodies. In fact, a sequence proximal to region 14 has been proposed in chicken CD3-⑀ as the site for antibody CT3 recognition (24).…”