Effect of infection of turkeys with haemorrhagic enteritis adenovirus isolate on the selected parameters of cellular immunity and the course of colibacillosis

Koncicki, A.; Tykałowski, Bartłomiej; Stenzel, Tomasz; Smiałek, M; Pestka, Daria

doi:10.2478/v10181-011-0136-2

Cited by 23 publications

(29 citation statements)

References 14 publications

(15 reference statements)

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“…Peripheral blood mononuclear cells (PBMCs) were isolated according to a previously described procedure (Koncicki et al 2012. The isolation of mononuclear cells from the spleen, thymus, bursa of Fabricius and CTs, and the determination of the percentages of CD4 + ,CD4 + /CD8 + , CD8 + T cell and IgM+ B cell subpopulations in PBMCs and selected organs of the immune system were carried out as described by Kubińska et al (2014).…”

Section: Flow Cytometrymentioning

confidence: 99%

“…The monoclonal (anti CD4 and CD8a) and polyclonal (anti IgM) antibodies used in the present experiment were originally developed to study the immune system of chickens, but they had been tested for cross-reactivity with turkeys, and the results of those tests were published by numerous authors (Van Nerom et al 1997, Li et al 1999a, Koncicki et al 2012.…”

Section: Immunological Parametersmentioning

confidence: 99%

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Biochemical and immunological responses of young turkeys to vaccination against Ornithobacterium rhinotraheale and different levels of dietary methionine

Kubińska¹,

Tykałowski²,

Koncicki³

et al. 2015

Polish Journal of Veterinary Sciences

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The objective of this study was to verify the hypothesis that increasing levels of dietary methionine can stimulate the mechanisms of cell-mediated and humoral immunity in young turkeys. The blood and organs involved in cell-mediated and humoral immune responses were analyzed in 8-week-old turkeys that had been vaccinated against Ornithobacterium rhinotraheale (ORT) infection (on days 17 and 48). The birds were fed diets with a low (LM), medium (MM) and high (HM) methionine content (0.45 and 0.40%, 0.60 and 0.51%, 0.71 and 0.57% in weeks 1 -4 and 5 -8, respectively).Dietary methionine supplementation led to a significant increase in body weights of turkeys at 56 days of age, from 3532 g in group LM to 3720 g in group MM and 3760 g in group HM (p=0.001). A significant increase in vaccine-induced antibody titers against ORT was noted in group HM relative to group LM (p=0.006). Increasing levels of methionine had no significant effect on total serum IgG nor IgM levels and most serum biochemical parameters, TP, ALB, GLOB, GLU, AST, ALP, P and Ca. In comparison with group LM, group HM turkeys were characterized by a lower percentage of IgM + B cell subpopulation in the blood and bursa of Fabricius.The percentages of CD4 + and CD8 + T cell subpopulations in the bursa of Fabricius in group HM were significantly different from those found in groups LM and MM. The highest percentages of CD4 + T cells and CD8 + T cells in the spleen were observed in groups LM (p<0.001) and HM (p=0.04), respectively. The differences were statistically significant relative to the remaining groups. Turkeys of group LM were characterized by a lower CD4 + T cell percentage in the thymus (p<0.001) and a lower CD8 + T cell percentage in the cecal tonsils (CTs) (p<0.01).Vaccination against ORT resulted in a significant increase in the percentage of CD4 + /CD8 + T cell subpopulation and a decrease in the percentage of CD8 + T cell subset in the spleen.

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Section: Flow Cytometrymentioning

confidence: 99%

Section: Immunological Parametersmentioning

confidence: 99%

Biochemical and immunological responses of young turkeys to vaccination against Ornithobacterium rhinotraheale and different levels of dietary methionine

Kubińska¹,

Tykałowski²,

Koncicki³

et al. 2015

Polish Journal of Veterinary Sciences

Self Cite

View full text Add to dashboard Cite

show abstract

“…The monoclonal (anti-CD4 and CD8a) and polyclonal (anti-IgM) antibodies used in the present experiment were originally developed to study the immune system of chickens, but they had been tested for cross-reactivity with turkeys, and the results of those tests were published by numerous authors (Van Nerom et al 1997, Li et al 1999, Koncicki et al 2012, Tykałowski et al 2014.…”

Section: Immunological Parametersmentioning

confidence: 99%

“…Commercially available fluorochrome-labeled monoclonal and polyclonal antibodies that recognize specific receptors and molecules on the surface and inside the immunocompetent cells are designed for flow cytometry detection of quantitative and qualitative changes in lymphocyte populations and subpopulations in the blood and organs of birds during the ongoing disease process (Koncicki et al 2012). Flow cytometry also allows the assessment of the functional status of various cell subpopulations in response to feed additives, drugs, immunosuppressants and immunostimulants (Tykałowski et al 2014).…”

Section: Immunological Parametersmentioning

confidence: 99%

“…Peripheral blood mononuclear cells (PBMCs) were isolated according to a previously described procedure (Koncicki et al 2012, Tykałowski et al 2014. Collected organs: spleen, thymus, bursa of Fabricius and cecal tonsils were individually homogenized in 1 ml RPMI-1640 medium (Sigma-Aldrich, Germany) with 5% FCS and centrifuged, using an automated tissue homogenizer (TissueLyser II, Qiagen, Germany).…”

Section: Isolation Of Mononuclear Cells and Flow Cytometrymentioning

confidence: 99%

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Immunological and biochemical indicators in turkeys fed diets with a different Methionine content

Kubińska¹,

Tykałowski²,

Jankowski³

et al. 2014

Polish Journal of Veterinary Sciences

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The objective of this study was to determine the effect of different levels of dietary methionine on selected immune parameters of young turkeys. A total of 357 one-day-old female Hybrid Converter turkeys were randomly divided into three groups with low, medium and high level of dietary methionine (LM, MM, HM) of seven replicates each. Methionine was added to the basal diet (LM) at 0.16% (MM) and 0.32% (HM). At 17 days of age, seven birds per group were vaccinated against Ornithobacterium rhinotraheale (ORT) infection, with ORNITIN (ABIC) vaccine. At 28 days of age, blood was sampled and the birds were euthanized. The serum concentrations and activity of selected biochemical parameters, total IgY and IgM, and vaccine-induced antibody titers (IgY) against ORT were determined. The percentages of CD4 + , CD8 + , CD4 + /CD8 + T cell subpopulations and IgM

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Adenovirus Infections

Fitzgerald¹,

Rautenschlein²,

Mahsoub³

et al. 2019

Diseases of Poultry

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Effect of infection of turkeys with haemorrhagic enteritis adenovirus isolate on the selected parameters of cellular immunity and the course of colibacillosis

Cited by 23 publications

References 14 publications

Biochemical and immunological responses of young turkeys to vaccination against Ornithobacterium rhinotraheale and different levels of dietary methionine

Biochemical and immunological responses of young turkeys to vaccination against Ornithobacterium rhinotraheale and different levels of dietary methionine

Immunological and biochemical indicators in turkeys fed diets with a different Methionine content

Adenovirus Infections

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