Identification and analysis of olfactory genes in Dioryctria abietella based on the antennal transcriptome

Transcriptome Analysis and Characterization of Chemosensory Genes in the Forest Pest, Dioryctria abietella (Lepidoptera: Pyralidae)

“…In comparison with a previous study on chemosensory genes in this species identified from the antennal transcriptome (15 OBPs, 18 CSPs, 42 ORs, 4 GRs, and 14 IRs) (Xing et al, 2021), our current transcriptome did not retrieve one CSP gene, namely DabiCSP2, but newly identified 140 genes encoding 27 OBPs, 6 CSPs, 33 ORs, 58 GRs, and 16 IRs. Of the identified OBPs and CSPs, we followed the previous nomenclature systems, except for DabiPBP3, GOBP1, and GOBP2 that were designated according to their orthology to OBPs in other lepidopterans.…”

“…Such sequencing and analysis yielded a low error rate (0.03%), high Phred values (Q20 = 98.26% and Q30 = 94.69%) and a moderate GC content (45.24%) (Table 1). With an emphasis on the antennae responsible for olfaction, here our transcriptome sequencing obtained more raw data from D. abietella antennae (females: 58,464,276 sequences and males: 71,979,886 sequences) compared to previous studies in D. abietella (47,005,976 and 50,818,590 reads for females and males, respectively) (Xing et al, 2021), and other two pyralid moths, G. mellonella (41,665,706 and 37,238,352 reads for females and males, respectively) (Zhao et al, 2019) and Plodia interpunctella (21,937,207 and 22,682,896 reads for females and males, respectively) (Jia et al, 2018). This may explain, to some extent, why D. abietella here possessed a larger number of ORs as members of this gene family were primarily enriched in the antennae (70 ORs in this study, 42 ORs in a previous study from D. abietella, 46 ORs in G. mellonella and 47 ORs in P. interpunctella) (Jia et al, 2018;Zhao et al, 2019;Xing et al, 2021).…”

“…For the identification of chemosensory genes in D. abietella, two de novo transcriptomes were used, i.e., the transcript transcriptome and the unigene transcriptome. First, a homologybased search with TBLASTN, implemented in Geneious v10.1.3 1 , was employed to identify genes from the transcriptomes, with queries of chemosensory genes from D. abietella, Galleria mellonella, Ostrinia furnacalis, and Bombyx mori (Zhang T. et al, 2015;Guo et al, 2017;Liu et al, 2018;Zhao et al, 2019;Jiang et al, 2021;Xing et al, 2021;Yin et al, 2021). In search of each query, we set e-value and maximum blast hits as 1e −5 and 20, respectively.…”

“…Of these, 35 HarmORs previously characterized were included (Liu et al, 2013;Jiang et al, 2014;Guo et al, 2020;Yang and Wang, 2021). The IRs from B. mori, D. abietella, H. armigera and O. furnacalis were used to infer the tree (Zhang T. et al, 2015;Liu et al, 2018;Xing et al, 2021). In the GR tree, we selected the GRs from B. mori, D. abietella and H. armigera, in which 15 representatives in H. armigera (CO 2 , sugar, GR43a-like and some bitter GRs known ligands) were included (Xu et al, 2016;Guo et al, 2017).…”

“…However, little is known about molecular mechanisms underlying intraspecific communication between female and male moths, as well as interspecific interaction between this pest and host plants. More recently, Xing et al (2021) described six chemosensory gene repertoires from D. abietella by antennal transcriptome analysis (15 OBPs, 18 CSPs, 65 ORs, 5 GRs, 24 IRs and 5 SNMPs), but the majority of which were partial sequences. Moreover, after our transcriptome-wide sequencing and comparative analyses of 16 tissues including the antennae in this species, the numbers of ORs, GRs, IRs, and SNMPs reported in the previous study were summed down to 42, 4, 14, and 3, respectively.…”