Identification and analysis of long non-coding RNAs that are involved in inflammatory process in response to transmissible gastroenteritis virus infection

Abstract

Background: Transmissible gastroenteritis virus (TGEV) infection can activate the immune response and cause inflammation. Long noncoding RNAs (lncRNAs) play important roles in antiviral innate immune response. However, whether lncRNAs participate in TGEV-induced inflammation in porcine intestinal epithelial cells (IPECs) is largely unknown. Results: In this study, the next-generation sequencing (NGS) technology was used to analyze the profiles of lncRNAs in Mock and TGEV-infected porcine intestinal epithelial cell-jejunum 2 (IPEC-J2) cell line. A total of 106 lncRNAs were differentially expressed. Many differentially expressed lncRNAs act as elements to competitively attach miRNAs with mRNAs to mediate expression of genes that related to Toll-like receptor, NOD-like receptor, TNF, and RIG-I-like receptor pathways. Functional analysis of the binding proteins and the up/down-stream genes of the differentially expressed lncRNAs revealed that lncRNAs were principally related to immune response. Meanwhile, we found that the differentially expressed lncRNA TCONS_00058367 might lead to a reduction of p-p65 in TGEV-infected IPEC-J2 cells by negatively regulating its antisense gene PML. Conclusions: The data showed that differentially expressed lncRNAs might be involved in immune response induced by TGEV through acting as miRNA sponges, regulating their up/down-stream genes, or directly binding proteins.

Diverse roles of long non‐coding RNAs in viral diseases

“…For example, differentially expressed lncRNAs affect transmissible gastroenteritis virus (TGEV)-induced inflammation through modulation of the NF-κB pathway. 88 Virus binding and entry are the first steps of viral infection.…”