Identificação dos genes Ty-2 e Ty-3 de resistência a begomovírusem genótipos de tomateiro

Abstract

O objetivo deste trabalho foi identificar a presença dos genes Ty-2 e Ty-3, de resistência a begomovírus, em acessos de tomateiro do Banco de Germoplasma de Hortaliças da Universidade Federal de Viçosa. Os oligonucleotídeos TO302 F/R e FLUW25 F/R foram utilizados em reações de PCR, para verificar a presença de marcadores relacionados aos genes Ty-2 e Ty-3, respectivamente. Observou-se a presença do gene Ty-2, em heterozigose na subamostra BGH-6881 (Solanum peruvianum), e do gene Ty-3, em homozigose nas subamostras BGH-6878, BGH-6897 (S. lycopersicum) e em heterozigose na subamostra BGH-6881. A identificação dos genes de resistência, com reações de PCR, representa um avanço para os programas de melhoramento de tomateiro no Brasil.

Evaluation of Datura stramonium and Nicandra physaloides as reservoirs of tomato severe rugose virus and whiteflies

“…There are several genes reported to confer resistance to tomato plants for begomoviruses, such as Ty‐1 , Ty‐2 , Ty‐3 , Ty‐4 , Ty‐5 , ty‐5 , tcm‐1 , and tgr‐1 . Initially, these genes were developed to reduce the impact of TYLCV, a monopartite begomovirus (Inoue‐Nagata et al , ); however, as ToSRV is a bipartite species, these genes do not confer a high resistance to the Brazilian virus, which can also lead to great losses in high inoculum pressure situations (Boiteux et al , ; Aguilera et al , ). Additionally, another technique adopted in order to reduce the inoculum source of ToSRV is to establish a crop‐free period (Salati et al , ).…”

“…When tomato is prematurely infected, symptoms and losses caused by the disease are higher (Giordano et al, 2005), and can reach 100% (Bergamin-Filho et al, 2016). It is also (Boiteux et al, 2007;Aguilera et al, 2011). Additionally, another technique adopted in order to reduce the inoculum source of ToSRV is to establish a crop-free period (Salati et al, 2002).…”

A Temporal Diversity Analysis of Brazilian Begomoviruses in Tomato Reveals a Decrease in Species Richness between 2003 and 2016

“…Traditionally, the most common preventive measures for begomoviral disease control are the application of insecticides against the vector and the use of resistant cultivars (Lapidot and Friedmann, 2002;Hurtado et al, 2012). To date, genes of the series Ty-1 to Ty-6 (and a few others such as tcm-1 and ty-5) were reported to provide resistance/tolerance to tomato yellow leaf curl virus (TYLCV), the most widespread begomovirus in the world (e.g., Zamir et al, 1994;Giordano et al, 2005;Anbinder et al, 2009;Ji et al, 2009;Hutton et al, 2012;Bai et al, 2018;Gill et al, 2019); some of these genes can provide a moderate level of control against NW begomoviruses (Giordano et al, 2005;Boiteux et al, 2007;Aguilera et al, 2011). For example, an experimental heterozygous hybrid carrying Ty-1 was less infected and displayed milder or no symptom under infection of the Brazilian begomoviruses tomato rugose mosaic virus (ToRMV) and tomato yellow vein streak virus (ToYVSV), and thus classified as tolerant to these viruses (Boiteux et al, 2007).…”