Identificação do circovírus suíno tipo 2 por reação em cadeia da polimerase e por imunoistoquímica em tecidos suínos arquivados desde 1988 no Brasil

Zanella, J. R. C.; Morés, N.; Simon, N. L.; Oliveira, Salete Rodrigues de; Gava, Danielle

doi:10.1590/s0103-84782006000500021

Cited by 14 publications

(7 citation statements)

References 13 publications

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“…The feasibility of more effective laboratory techniques for the detection of PCV2 is essential. The literature reports the identification of PCV2 DNA sequences by molecular techniques such as in situ hybridization and PCR (Larochelle et al., ; Kim and Chae, ; Ciacci‐Zanella et al., ; Vilcek et al., ). In this study, we developed and evaluated the STNPCR for the detection of PCV2 DNA.…”

Section: Discussionmentioning

confidence: 99%

Development and Evaluation of Single-tube Nested PCR (STNPCR) for the Detection of Porcine Circovirus type 2 (PCV2)

Pontes

Barbosa

Jesus

et al. 2012

Transbound Emerg Dis

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Porcine circovirus 2 (PCV2) is a common virus in pig population and is associated with the postweaning multisystemic wasting disease (PMWS). In this study, it was developed and evaluated the single-tube nested PCR (STNPCR) method for the detection of PCV2 DNA. PCV2 reference controls and swine tissue samples were used, and primers were selected for targeting specific regions of the viral genome. In comparison of the methods, STNPCR was 10 times more sensitive than conventional PCR and showed the same sensitivity to nested PCR (NPCR), but with reduction in the risk of cross-contamination. In clinical application, 55 tissue samples were analysed by conventional PCR and resulted in 67% (37/55) of positive reactions, while the NPCR and STNPCR were able to identify the presence of viral DNA in 100% (55/55) of the samples. The high sensitivity combined with the elimination of cross-contamination makes the STNPCR method suitable for the epidemiological studies of PCV2 and can aid in the diagnosis of PMWS.

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Section: Discussionmentioning

confidence: 99%

Development and Evaluation of Single-tube Nested PCR (STNPCR) for the Detection of Porcine Circovirus type 2 (PCV2)

Pontes

Barbosa

Jesus

et al. 2012

Transbound Emerg Dis

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“…In order to verify the absence of other primary respiratory pathogens, the IHC test was carried out for PCV2 (Ciacci-Zanella et al 2006), influenza A virus (Vincent et al 1997) and M. hyopneumoniae in all lung samples. For PCV2 the test was also performed in mediastinal lymph nodes.…”

Section: Methodsmentioning

confidence: 99%

Pasteurella multocida type A as the primary agent of pneumonia and septicaemia in pigs

et al. 2015

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In order to understand better the pathological aspects and spread of Pasteurella multocida type A as the primary cause of pneumonia in pigs, was made an experiment with intranasal inoculation of different concentrations of inocula [Group (G1): 108 Colony Forming Units (CFU)/ml; G2: 107 CFU/ml; G3: 106 CFU/ml and G4: 105 CFU/ml], using two pigs per group. The pigs were obtained from a high health status herd. Pigs were monitored clinically for 4 days and subsequently necropsied. All pigs had clinical signs and lesions associated with respiratory disease. Dyspnoea and hyperthermia were the main clinical signs observed. Suppurative cranioventral bronchopneumonia, in some cases associated with necrosuppurative pleuropneumonia, fibrinous pericarditis and pleuritic, were the most frequent types of lesion found. The disease evolved with septicaemia, characterized by septic infarctions in the liver and spleen, with the detection of P. multocida type A. In this study, P. multocida type A strain #11246 was the primary agent of fibrinous pleuritis and suppurative cranioventral bronchopneumonia, pericarditis and septicaemia in the pigs. All concentrations of inoculum used (105-108 CFU/ml) were able to produce clinical and pathological changes of pneumonia, pleuritis, pericarditis and septicemia in challenged animals.

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“…Samples of tissues embedded in paraffin were sectioned at 4μm, placed on slides (previously treated with 10% poly-L-lysine) and IHC was performed as described previously (Gava et al 2005a). Controls for the analyses includes samples of lymph nodes collected from Specific Pathogen Free pigs (SPF) and from PMWS positive pigs, previously tested by HE and nested-PCR (Ciacci-Zanella & Mores 2003, Ciacci-Zanella et al 2006. Sections were deparaffinized in xylene and rehydrated in decreasing graded alcohol solutions (100, 96, 80 and 70%).…”

Section: Methodsmentioning

confidence: 99%

“…However, retrospective studies indicated the presence of PCV2 and PMWS lesions since 1988 (Ciacci-Zanella et al 2006). Subsequently, the disease has been described in several other states and in other countries; and both PCV1 and PCV2 circulate within the Brazilian swine population (Castro et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Transmission of porcine circovirus 2 (PCV2) by semen and viral distribution in different piglet tissues

et al. 2008

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RESUMO.-[Transmissão do circovirus suíno 2 (PCV2) pelo sêmen e distribuição do vírus nos tecidos da leitegada.] A circovirose suína é causada pelo circovirus suíno 2 (PCV-2). Dentre seis diferentes manifestações clí-nicas envolvendo sinais respiratórios, entéricos, nervosos e reprodutivos, a síndrome multissistêmica do definhamento do suíno (SMDS) é a doença mais importante e estudada. Todavia, relatos sobre falhas reprodutivas associadas ao PCV2 tem aumentado. Alguns estudos mostraram a possibilidade de contaminação das fêmeas pelo sêmen de machos positivos para PCV2. Porcine circovirus infections are caused by the porcine circovirus 2 (PCV2). Among six different clinical manifestations involving respiratory, enteric, nervous and reproductive signs, the postweaning multisystemic wasting syndrome (PMWS) is the most important and studied disease. However, reproductive failures associated with PCV2 have been increasingly reported. Some studies have shown the possible contamination of sows by semen of PCV2 positive boars. In order to investigate the transmission of PCV2 by contaminated semen and its ability to infect the sow and piglets, 20 PCV2 negative sows were inseminated, 10 with negative boar semen and 10 with previously nested-PCR tested positive boar semen. The sows were weekly monitored and blood samples were collected. Based on the results, 4 out 20 sows were selected (1 sow was PCR negative and inseminated with a negative semen, 2 sows were PCR negative and inseminated with a positive semen and 1 sow was PCR negative and inseminated with a positive semen, but became PCR positive around the 30 days of pregnancy). After weaning, 12 male piglets, 3 of each sow, were selected and maintained under isolation. In order to investigate which organs harbored the virus, the young pigs were necropsied around 9 months of age. Samples of serum collected monthly were tested by immunocitochemistry (ICC), and all 12 pigs serum converted. Samples of lymphoid, systemic and reproductive organs were analyzed by nested-PCR and immunohistochemistry (IHC). Evaluation of the samples by nested-PCR, revealed that several tissues were positive in 10 of 12 pigs, mainly the lymph nodes, bone marrow and spleen. Various samples were positive by IHC in 8 of 12 piglets, being the lymph nodes, tonsils and bulbourethral glands the most frequently positive. Thus, the results of testing different samples, in the 3 tests (ICC, nested-PCR and IHC) were complementary. These results show that PCV2 transmission through semen to the sows and piglets may occur and may also represent a potential risk for the herd.

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Identificação do circovírus suíno tipo 2 por reação em cadeia da polimerase e por imunoistoquímica em tecidos suínos arquivados desde 1988 no Brasil

Cited by 14 publications

References 13 publications

Development and Evaluation of Single-tube Nested PCR (STNPCR) for the Detection of Porcine Circovirus type 2 (PCV2)

Development and Evaluation of Single-tube Nested PCR (STNPCR) for the Detection of Porcine Circovirus type 2 (PCV2)

Pasteurella multocida type A as the primary agent of pneumonia and septicaemia in pigs

Transmission of porcine circovirus 2 (PCV2) by semen and viral distribution in different piglet tissues

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