ICTV Virus Taxonomy Profile: Ophioviridae

García, María Laura; Bó, E. Dal; Graça, J. V. da; Gago‐Zachert, Selma; Moreno, Pedro; Natsuaki, Tomohide; Pallás, Vicente; Navarro, J. A.; Reyes, Carina A.; Luna, Gabriel Robles; Sasaya, Takahide; Tzanetakis, Ioannis E.; Vaira, A. M.; Verbeek, M.

doi:10.1099/jgv.0.000836

Cited by 29 publications

(20 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ophioviruses (family Ophioviridae) are a recently established family of viruses 2 that infects plants, causing economically important diseases [1,2]. Only one genus 3 (Ophiovirus) is currently recognized, containing seven species ( Table 1).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of the first endogenous Ophiovirus sequence

Marsile-Medun

Debat

Gifford

2017

Preprint

View full text Add to dashboard Cite

Endogenous viral elements (EVEs) are sequences in eukaryotic genomes that are derived from the ancestral integration of viral sequences into germline cells.Ophioviruses (family Ophioviridae) are a recently established family of viruses that infects plants. In this report, we describe the first example of an EVE derived from an ophiovirus, in the genome of eelgrass (Zostera marina). These findings extend the host range of ophioviruses to include seagrasses of the family Zosteraceae.

show abstract

Section: Introductionmentioning

confidence: 99%

“…while the coat protein (CP) is encoded by the third. A fourth segment has been 10 reported in ophioviruses infecting lettuce (Lactuca sativa), which encodes putative 11 proteins of unknown function [1]. 12…”

Section: Introductionmentioning

confidence: 99%

Identification of the first endogenous Ophiovirus sequence

Marsile-Medun

Debat

Gifford

2017

Preprint

View full text Add to dashboard Cite

show abstract

“…The RsAVL2 partial ORF encodes RdRp (Pfam00978, E-value 2.9e-21), whereas both RdRp (Pfam00978, E-values 3.8e-18 and 2.1e-19, respectively) and viral helicase (Pfam01443, E-values 8.5e-05 and 6.6e-11, respectively) domains can be detected in RsAVL1 and RsAVL3 [6]. A new family Mycoalphaviridae was proposed to accommodate RsALV1, RsALV 2, and RsALV3, together with Rhizoctonia solani RNA virus 1, 2, and 3 (RsRV1-3/DC17; Table S1) Aspiviridae, formerly known as Ophioviridae, is a family of flexible filamentous viruses known to infect plants [121] and belongs to the order Serpentovirales. The family Aspiviridae currently accommodates one genus Ophiovirus and 7 species.…”

Section: (+)Ssrna Viruses: Mycoalphaviridaementioning

confidence: 99%

“…The family Aspiviridae currently accommodates one genus Ophiovirus and 7 species. The members of the family Aspirividae contain a (−)ssRNA genome ranging from 11.3 to 12.5 kb in length separated into 3 to 4 segments [121]. Recently, unclassified partial virus sequences related to ophioviruses were reported infecting soil-borne R. solani strains and named Rhizoctonia solani negative-stranded RNA virus 1 to 3 (RsNSRV1-3; Table 2).…”

Section: (+)Ssrna Viruses: Mycoalphaviridaementioning

confidence: 99%

Viruses Infecting the Plant Pathogenic Fungus Rhizoctonia solani

Abdoulaye

Foda

Kotta‐Loizou

2019

Viruses

View full text Add to dashboard Cite

The cosmopolitan fungus Rhizoctonia solani has a wide host range and is the causal agent of numerous crop diseases, leading to significant economic losses. To date, no cultivars showing complete resistance to R. solani have been identified and it is imperative to develop a strategy to control the spread of the disease. Fungal viruses, or mycoviruses, are widespread in all major groups of fungi and next-generation sequencing (NGS) is currently the most efficient approach for their identification. An increasing number of novel mycoviruses are being reported, including double-stranded (ds) RNA, circular single-stranded (ss) DNA, negative sense (−)ssRNA, and positive sense (+)ssRNA viruses. The majority of mycovirus infections are cryptic with no obvious symptoms on the hosts; however, some mycoviruses may alter fungal host pathogenicity resulting in hypervirulence or hypovirulence and are therefore potential biological control agents that could be used to combat fungal diseases. R. solani harbors a range of dsRNA and ssRNA viruses, either belonging to established families, such as Endornaviridae, Tymoviridae, Partitiviridae, and Narnaviridae, or unclassified, and some of them have been associated with hypervirulence or hypovirulence. Here we discuss in depth the molecular features of known viruses infecting R. solani and their potential as biological control agents.

show abstract

“…Citrus psorosis virus (CPsV), the type member of the Aspiviridae family (formerly Ophioviridae), genus Ophiovirus (40,41), is a three-partite, nonenveloped, negativesense, single-stranded RNA (ssRNA) virus. RNA 1 encodes a 280-kDa replicase (42) as well as a 24-kDa protein that affects microRNA (miRNA) maturation (43) and has RNA silencing-suppressing activity (44).…”

mentioning

confidence: 99%

Citrus Psorosis Virus Movement Protein Contains an Aspartic Protease Required for Autocleavage and the Formation of Tubule-Like Structures at Plasmodesmata

Luna

Peña

Borniego

et al. 2018

J Virol

Self Cite

View full text Add to dashboard Cite

Plant virus cell-to-cell movement is an essential step in viral infections. This process is facilitated by specific virus-encoded movement proteins (MPs), which manipulate the cell wall channels between neighboring cells known as plasmodesmata (PD). Citrus psorosis virus (CPsV) infection in sweet orange involves the formation of tubule-like structures within PD, suggesting that CPsV belongs to "tubule-forming" viruses that encode MPs able to assemble a hollow tubule extending between cells to allow virus movement. Consistent with this hypothesis, we show that the MP of CPsV (MP) indeed forms tubule-like structures at PD upon transient expression in leaves. Tubule formation by MP depends on its cleavage capacity, mediated by a specific aspartic protease motif present in its primary sequence. A single amino acid mutation in this motif abolishes MP cleavage, alters the subcellular localization of the protein, and negatively affects its activity in facilitating virus movement. The amino-terminal 34-kDa cleavage product (34K), but not the 20-kDa fragment (20K), supports virus movement. Moreover, similar to tubule-forming MPs of other viruses, MP (and also the 34K cleavage product) can homooligomerize, interact with PD-located protein 1 (PDLP1), and assemble tubule-like structures at PD by a mechanism dependent on the secretory pathway. 20K retains the protease activity and is able to cleave a cleavage-deficient MP in Altogether, these results demonstrate that CPsV movement depends on the autolytic cleavage of MP by an aspartic protease activity, which removes the 20K protease and thereby releases the 34K protein for PDLP1-dependent tubule formation at PD. Infection by citrus psorosis virus (CPsV) involves a self-cleaving aspartic protease activity within the viral movement protein (MP), which results in the production of two peptides, termed 34K and 20K, that carry the MP and viral protease activities, respectively. The underlying protease motif within the MP is also found in the MPs of other members of the family, suggesting that protease-mediated protein processing represents a conserved mechanism of protein expression in this virus family. The results also demonstrate that CPsV and potentially other ophioviruses move by a tubule-guided mechanism. Although several viruses from different genera were shown to use this mechanism for cell-to-cell movement, our results also demonstrate that this mechanism is controlled by posttranslational protein cleavage. Moreover, given that tubule formation and virus movement could be inhibited by a mutation in the protease motif, targeting the protease activity for inactivation could represent an important approach for ophiovirus control.

show abstract

ICTV Virus Taxonomy Profile: Ophioviridae

Cited by 29 publications

References 6 publications

Identification of the first endogenous Ophiovirus sequence

Identification of the first endogenous Ophiovirus sequence

Viruses Infecting the Plant Pathogenic Fungus Rhizoctonia solani

Citrus Psorosis Virus Movement Protein Contains an Aspartic Protease Required for Autocleavage and the Formation of Tubule-Like Structures at Plasmodesmata

Contact Info

Product

Resources

About