ICP-MS for the neurodegenerative and brain sciences

Ha, Yonghwang; Tsay, Olga G.; Churchill, David G.

doi:10.1007/978-3-7091-1001-0_19

Cited by 1 publication

(1 citation statement)

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“…In light of this, laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS), as a means of ultra-sensitive chemical analysis with a part per billion detection limit, has received much attention for visualizing metals in biological systems, such as intact samples and tissue sections [ 21 ]. LA-ICP-MS has been applied for quantitative imaging of Cu, Fe, Zn, and Mn in the MPTP mouse model of Parkinson’s disease [ 22 ]; measuring the Mn, Fe, Cu, and Zn concentrations in the brain of a rat model of Parkinson’s disease [ 23 ]; copper mapping in a zebrafish model of Menkes disease [ 24 ]; imaging of Cu, Zn, Pb, and U in human brain tumour resections [ 25 ]; measuring Zn and Fe concentrations in the mouse model of traumatic brain injury (TBI) [ 26 ]; and imaging of iron in the frontal cortex [ 27 ], hippocampus [ 28 ], and white and grey matter [ 29 ] of healthy controls and Alzheimer’s disease patients.…”

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confidence: 99%

Distribution of Copper, Iron, and Zinc in the Retina, Hippocampus, and Cortex of the Transgenic APP/PS1 Mouse Model of Alzheimer’s Disease

Hosseinpour-Mashkani

Bishop

Raoufi-Rad

et al. 2023

Cells

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A mis-metabolism of transition metals (i.e., copper, iron, and zinc) in the brain has been recognised as a precursor event for aggregation of Amyloid-β plaques, a pathological hallmark of Alzheimer’s disease (AD). However, imaging cerebral transition metals in vivo can be extremely challenging. As the retina is a known accessible extension of the central nervous system, we examined whether changes in the hippocampus and cortex metal load are also mirrored in the retina. Laser ablation inductively coupled plasma-mass spectrometry (LA-ICP-MS) was used to visualise and quantify the anatomical distribution and load of Cu, Fe, and Zn in the hippocampus, cortex, and retina of 9-month-old Amyloid Precursor Protein/Presenilin 1 (APP/PS1, n = 10) and Wild Type (WT, n = 10) mice. Our results show a similar metal load trend between the retina and the brain, with the WT mice displaying significantly higher concentrations of Cu, Fe, and Zn in the hippocampus (p < 0.05, p < 0.0001, p < 0.01), cortex (p < 0.05, p = 0.18, p < 0.0001) and the retina (p < 0.001, p = 0.01, p < 0.01) compared with the APP/PS1 mice. Our findings demonstrate that dysfunction of the cerebral transition metals in AD is also extended to the retina. This could lay the groundwork for future studies on the assessment of transition metal load in the retina in the context of early AD.

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