ICI 182,780 acts as a partial agonist and antagonist of estradiol effects in specific cells of the sheep uterus

Robertson, Jane A.; Zhang, Yuhua; Ing, Nancy H.

doi:10.1016/s0960-0760(01)00061-9

Cited by 39 publications

(28 citation statements)

References 22 publications

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“…Our results suggest that expression of a relatively high level of PRB might be associated with tamoxifen and/or ICI 182,780 resistance in human breast tumors and may involve induction of VEGF to support tumor growth. This is consistent with the observation that tamoxifen is an agonist for estrogen-stimulated YB xenografts (30) and that ICI-182,780 can also function as an agonist in a cell-specific manner (37,39).…”

Section: Resultssupporting

confidence: 91%

Progestin-Dependent Induction of Vascular Endothelial Growth Factor in Human Breast Cancer Cells

Richer

Horwitz

et al. 2004

Cancer Research

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Section: Resultssupporting

confidence: 91%

Progestin-Dependent Induction of Vascular Endothelial Growth Factor in Human Breast Cancer Cells

Richer

Horwitz

et al. 2004

Cancer Research

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“…The same low dose of E 2 (10 9 M) that increased expression of pERE 2 tkCAT in transfected Ishikawa cells also up-regulated ER mRNA in untransfected cells. The magnitude of the E 2 -induced increase in ER mRNA levels in Ishikawa cells is consistent with that in the endometrium of ovariectomized ewes in response to a physiological dose of E 2 (Robertson et al 1998, Zou & Ing 1998, Robertson et al 2001). E 2 levels of 10 9 and 10 8 M have been used to up-regulate expression of the PR gene and other genes in Ishikawa cells (Holinka et al 1986, Jamil et al 1991, Lessey et al 1996, Pinelli et al 1998, Lovely et al 2000.…”

Section: Discussionsupporting

confidence: 72%

Estradiol up-regulates estrogen receptor messenger ribonucleic acid in endometrial carcinoma (Ishikawa) cells by stabilizing the message

Robertson

Farnell²,

Lindahl³

et al. 2002

Journal of Molecular Endocrinology

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Estrogens up-regulate expression of the estrogen receptor alpha (ER) gene in most mammalian tissues studied. Using the ovariectomized ewe as a model, we determined that estradiol (E 2 ) acted post-transcriptionally to increase endometrial ER mRNA concentrations by enhancing the stability of the message. The purpose of this study was to determine whether a similar E 2 effect occurs in Ishikawa cells, a well-differentiated human endometrial adenocarcinoma cell line. The presence and function of ER protein in Ishikawa cells was demonstrated by transactivation of a transfected plasmid (ERE 2 tkCAT) in response to 10 −9 M E 2 , resulting in a 550% increase in reporter gene RNA. Ishikawa cells also responded to E 2 by up-regulating their ER mRNA concentration an average of 100% between 7 and 24 h of treatment. The effect of E 2 on ER mRNA stability was measured after blocking transcription with actinomycin D to find that the half-life increased from 6 to 10 h in control and E 2 -treated cells respectively. These results are consistent with cell-free studies which showed significant enhancement of the half-life of radiolabeled ER 3′ untranslated region (3′UTR) RNA in extracts from E 2 -treated cells versus those from control cells. Thus, Ishikawa cells provide a relevant model system for the study of E 2 -regulated endometrial gene expression.

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“…In vivo, ovine uterine cells responded to estrogen treatment by up-regulating the transcription of ERa mRNA (Ing and Tornesi, 1997;Robertson et al, 2001). Estrogen also increased ERa mRNA levels in ZR-75 (Clayton et al, 1997) and Ishikawa cells (Robertson et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells

Zhang¹,

Chen²,

Li³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Estrogen regulates reproductive behavior and drives the proliferation and differentiation of several cell types. These physiological functions of estrogen are mediated by estrogen receptors (ERs), and each ER isoform plays a distinct role. To clarify the molecular mechanism of estrogen action and to evaluate the effect of ERs on the secretion of ovalbumin (OVA) in pigeon oviduct epithelial cells (POECs), we determined the complete coding sequences encoding ER alpha (ERα) and ER beta (ERβ) in pigeons. The abundance of pigeon ERα and ERβ mRNA was detected using quantitative polymerase chain reaction. These results revealed that pigeon ERα is highly expressed in the oviduct, while pigeon ERb is highly expressed in the ovary and kidney. We hypothesize that ERα mRNA predominates over that of ERβ in the oviduct. The expression of ERα can be down- 1927©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (1): 1926-1937 (2014 Characterization and regulation of pigeon ERs regulated by 17β-estradiol, and the knockdown of ERα promoted OVA mRNA expression in cultured POECs, indicating that ERα may play an important role in OVA secretion.

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ICI 182,780 acts as a partial agonist and antagonist of estradiol effects in specific cells of the sheep uterus

Cited by 39 publications

References 22 publications

Progestin-Dependent Induction of Vascular Endothelial Growth Factor in Human Breast Cancer Cells

Progestin-Dependent Induction of Vascular Endothelial Growth Factor in Human Breast Cancer Cells

Estradiol up-regulates estrogen receptor messenger ribonucleic acid in endometrial carcinoma (Ishikawa) cells by stabilizing the message

Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells

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