Ichthyotoxic Phloroglucinol Derivatives from Dryopteris fragrans and Their Anti-tumor Promoting Activity.

Ichthyotoxic and Anticarcinogenic Effects of Triterpenoids from Sandoricum koetjape Bark

“…As shown in Table 1, compounds 1, 2, and 3 inhibited EBV-EA activation by 65.4, 70.1, and 64%, respectively, at a concentration of 500 mol ratio/TPA, preserving high viability of the cells. These potencies were comparable to those reported for oleanolic acid 17) and the well-known anti-tumor promoting agent EGCG 12,18) ( Table 1). Although many types of triterpenoids such as taraxastane-, multiflorane-, cucurbitane-, and oleanane-type have been reported to show an inhibitory effect on EBV-EA activation, 17,[19][20][21][22] the effect of the oleanane-type related triterpenoids, 17,[20][21][22] including compounds 1 and 2, appears to be more potent than that of the multiflorane-and cucurbitane-type tripterpenoids.…”

“…16) Our study provided additional evidence of the effectiveness of the ichthyotoxic assay in the search for bioactive natural products, although relationships between the ichthyotoxic activity and the other activities are not clear. The remarkable anti-tumor promoting effect of aspidin BB 12) prompted us to examine the anti-tumor effects of compounds 1 and 2 in vitro and in vivo.…”

“…12) Raji cells were incubated at 37°C for 48 h in a medium (1 ml) containing n-butyric acid (4 mM), TPA [20 ng (32 pM) in DMSO 2 ml] as an inducer, and a known amount of test compound in 5 ml DMSO. Smears were made from the cell suspensions, and the activated cells that were stained with EBV-EA-positive serum were detected using an indirect immunofluorescence technique.…”

Characterization of a New Sesquiterpene and Antifungal Activities of Chemical Constituents from Dryopteris fragrans (L.) Schott

“…The retention time was similar to that of a D-glucose derivative, so the sugar was identified as D-glucose. [8][9][10] M. canis and E. floccosum were obtained from the fungus preservation center in the China Academy of Sciences and the Institute of Medicine of Dermatology, respectively. Antifungal tests were performed using the method of Dilution Antifungal Susceptibility Testing of Filamentous Fungi as described by the National Committee for Clinical Laboratory Standards [9].…”

“…Previous research had discovered phloroglucins, terpenes, flavonoids, saponins, essential oils and sterols in this plant, and activity screenings of the related constituents have become popular [2,5,6]. Our research group has reported one new phenolic acid from the herb [7,8].…”