Icariin-loaded electrospun PCL/gelatin nanofiber membrane as potential artificial periosteum

Gong, Min; Chen, Cheng; Ye, Jingjing; Liao, Meihong; Xie, Weijun; Wu, Chengai; Shi, Rui; Zhang, Liqun

doi:10.1016/j.colsurfb.2018.06.012

Cited by 66 publications

(54 citation statements)

References 38 publications

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“…Wu et al showed that the ALP level of BMSCs cultured on new ICA-loaded hybrid hydroxyapatite granules with micro/nano pores was significantly enhanced [ 40 ]. Gong et al reported improved ALP and calcium levels in MC3T3-E1 cells cultured on an ICA-loaded polycaprolactone (PCL)/gelatin fibrous matrix via the release of ICA [ 45 ]. Those previous results are consistent with our results and suggest that ICA elevates ALP and calcium levels.…”

Section: Discussionmentioning

confidence: 99%

“…The expression of RUNX2/Id-1 was reported to be achieved by ICA in both pre-osteoblastic MC3T3-E1 cells and osteoblasts. Previous studies reported that ICA treatment not only enhanced COL I expression in both rBMSCs and MC3T3-E1 cells, but also increased the OPN level in MC3T3-E1 cells [ 16 , 23 , 45 ]. Recently, it was reported that miR-153 promoted the mRNA and protein levels of RUNX2 during the osteoblast differentiation of MC3T3-El cells [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

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Icariin-Functionalized Nanodiamonds to Enhance Osteogenic Capacity In Vitro

et al. 2020

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Nanodiamonds (NDs) have been used as drug delivery vehicles due to their low toxicity and biocompatibility. Recently, it has been reported that NDs have also osteogenic differentiation capacity. However, their capacity using NDs alone is not enough. To significantly improve their osteogenic activity, we developed icariin (ICA)-functionalized NDs (ICA-NDs) and evaluated whether ICA-NDs enhance their in vitro osteogenic capacity. Unmodified NDs and ICA-NDs showed nanosized particles that were spherical in shape. The ICA-NDs achieved a prolonged ICA release for up to 4 weeks. The osteogenic capacities of NDs, ICA (10 μg)-NDs, and ICA (50 μg)-NDs were demonstrated by alkaline phosphatase (ALP) activity; calcium content; and mRNA gene levels of osteogenic-related markers, including ALP, runt-related transcript factor 2 (RUNX2), collagen type I alpha 1 (COL1A1), and osteopontin (OPN). In vitro cell studies revealed that ICA (50 μg)-ND-treated MC3T3-E1 cells greatly increased osteogenic markers, including ALP, calcium content, and mRNA gene levels of osteogenic-related markers, including ALP, RUNX2, COL1A1, and OPN compared to ICA (10 μg)-NDs or ND-treated cells. These our data suggest that ICA-NDs can promote osteogenic capacity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Icariin-Functionalized Nanodiamonds to Enhance Osteogenic Capacity In Vitro

et al. 2020

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“…The architecture of electrospun nanofibrous made from natural biodegradable and biocompatible material (gelatin and chitosan) was similar to real tissue, which can satisfy physiological requirements. However, the strength properties of electro spun chitosan fibers are weak [19], and gelatin is sensitive to degradation [20,21]. In this work, the blending of chitosan with gelatin can improve the mechanical properties of chitosan fibers, what's more, chitosan is a hard electrospinning biomaterial, and gelatin can increase the spinnability of chitosan.…”

Section: Discussionmentioning

confidence: 87%

Construction of Sandwich-like cell sheets with natural electrospun gelatin/chitosan nanofibrous delivered plasmid VEGF for accelerated wound healing

Zhu

Liao

Zhang

et al. 2020

Preprint

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Background Development of natural biodegradable electrospun nanofibrous with appropriate physical properties and biocompatibility is highly desirable to support multi-layer cell sheets construction for wound healing. Results We developed a series of electrospun gelatin/chitosan nanofibrous with different gelatin/chitosan ratios and controlled pore sizes, and impregnated plasmid VEGF into membrane, which as supporting membrane to construct sandwich-like adipose-derived stem cells (ADSCs) cell sheets with a simple and effective technique for accelerated wound healing. We found that the physical properties of the electrospun nanofibrous including water retention, stiffness, strength, elasticity and degradation could be tailored by changing the proportion of gelatin/chitosan. We further observed that the optimized electrospun nanofibrous with the optimal ratio of gelatin to chitosan (7:3) which were soft and elastic could most effectively support cell adhesion, proliferation and migration into the whole nanofibrous membranes. Nanofibrous delivered plasmid VEGF facilitating multi-layer ADSC cell sheets formation and promoting regeneration of cutaneous tissues within two weeks. Conclusions Such natural biodegradable and biocompatible electrospun gelatin/chitosan nanofibrous with plasmid have the potential to become fully cellularized and support sandwich-like ADSC cell sheets formation, which will make it suitable for widespread applications such as skin substitute or wound dressing.

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“…The advantage of doing so was to regulate the dual release of ICA and Mg 2+ ion in a more controllable mode. ICA is poorly water‐soluble and slowly released from polymeric matrixes if it is embedded directly, [ 13 ] on the contrary, the degradation of MgO in water is fast due to its hydrophilicity. From Figure 1, it was found the ICA would like to adsorb onto MgO particles rather than onto MgCO 3 particles.…”

Section: Discussionmentioning

confidence: 99%

“…loaded ICA into electrospun polycaprolactone/gelatin composite fibers and found the fibrous mesh containing 0.05 wt% ICA able to promote expressions of various indicators as akaline phosphatase (ALP), collagen I (Col‐I), osteocalcin (OCN), and Ca 2+ deposition in relation to osteogenesis. [ 13 ] Lai et al. incorporated ICA into composite scaffolds made of poly(lactide‐ co ‐glycolide) (PLGA) and β ‐tricalcium phosphate ( β ‐TCP), and the scaffolds exhibited significant osteoinductivity in treating bone defects that were resulted from steroid‐associated osteonecrosis.…”

Section: Introductionmentioning

confidence: 99%

Dual‐Controlled Release of Icariin/Mg²⁺ from Biodegradable Microspheres and Their Synergistic Upregulation Effect on Bone Regeneration

Yuan

Wan

Wei

et al. 2020

Adv Healthcare Materials

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Current scaffolds applied for bone tissue engineering are still lacking sufficient osteogenic capacity to induce efficient bone regeneration. Biodegradable microsphere‐type scaffolds are designed to achieve the dual‐controlled release of a Chinese medicine (i.e., icariin, ICA) and a bioactive ion (i.e., Mg2+), in order to achieve their synergistic effect on inducing osteogenesis. The hydrophobic icariin is preloaded onto MgO/MgCO3 (1:1 in weight ratio) particles at different amounts and then the particles are encapsulated into biodegradable poly(lactide‐co‐glycolide) (PLGA) microspheres (PMI) at a fixed fraction (20 wt%). Continuous releases of Mg2+ ion and icariin from the microspheres are detected, showing dependence on icariin amounts. At an optimized moderate loading amount, the resulting PMI‐M microspheres display the strongest activation effect on cell biological behaviors among all the designs. By implanting the PMI‐M microspheres into rat calvarial defects for 16 weeks, it is found that they can effectively enhance new bone formation, presenting significantly higher capacity in inducing osteogenesis than PMg (containing MgO/MgCO3 but without icariin) and blank PLGA microspheres. Clearly, the released Mg2+ ions are beneficial to osteogenesis, and the coincorporation of icariin exerts supplemental effects in inducing new bone formation, which suggest a promising strategy to regenerate severe bone injuries by designing a dual‐release system.

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Icariin-loaded electrospun PCL/gelatin nanofiber membrane as potential artificial periosteum

Cited by 66 publications

References 38 publications

Icariin-Functionalized Nanodiamonds to Enhance Osteogenic Capacity In Vitro

Icariin-Functionalized Nanodiamonds to Enhance Osteogenic Capacity In Vitro

Construction of Sandwich-like cell sheets with natural electrospun gelatin/chitosan nanofibrous delivered plasmid VEGF for accelerated wound healing

Dual‐Controlled Release of Icariin/Mg²⁺ from Biodegradable Microspheres and Their Synergistic Upregulation Effect on Bone Regeneration

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Icariin-loaded electrospun PCL/gelatin nanofiber membrane as potential artificial periosteum

Cited by 66 publications

References 38 publications

Icariin-Functionalized Nanodiamonds to Enhance Osteogenic Capacity In Vitro

Icariin-Functionalized Nanodiamonds to Enhance Osteogenic Capacity In Vitro

Construction of Sandwich-like cell sheets with natural electrospun gelatin/chitosan nanofibrous delivered plasmid VEGF for accelerated wound healing

Dual‐Controlled Release of Icariin/Mg2+ from Biodegradable Microspheres and Their Synergistic Upregulation Effect on Bone Regeneration

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Dual‐Controlled Release of Icariin/Mg²⁺ from Biodegradable Microspheres and Their Synergistic Upregulation Effect on Bone Regeneration