Icariin enhances intestinal barrier function by inhibiting NF-κB signaling pathways and modulating gut microbiota in a piglet model

Xiong, Wen; Ma, Haoyue; Zhu, Zhiliang; Jin, Minghua; Wang, Jian; Xu, Yu; Wang, Zili

doi:10.1039/c9ra07176h

Cited by 22 publications

(27 citation statements)

References 61 publications

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“…The intestinal epithelial barrier includes a lining of enterocytes and intercellular multiprotein complexes (such as ZO-1, claudin and occludin), which plays a crucial role in preventing pathogenic bacteria, antigens and toxins from entering into the systemic circulation via the gut lumen( Chen et al., 2018 ; Li et al., 2019 ). The DAO, D-lactate and endotoxins in serum are useful markers of intestinal permeability ( Xiong et al., 2019 ). The present study showed that WB supplementation reduced the serum DAO activity compared with CON on d 14, suggesting decreased intestinal permeability and enhanced intestinal barrier function.…”

Section: Discussionmentioning

confidence: 99%

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Source of fiber influences growth, immune responses, gut barrier function and microbiota in weaned piglets fed antibiotic-free diets

Shang

Liu

et al. 2021

Animal Nutrition

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This study examined the impacts of different fiber sources on growth, immune status and gut health in weaned piglets fed antibiotic-free diets. Sixty piglets (BW = 8.18 ± 1.35 kg) were assigned to 3 dietary treatments based on BW and gender in a randomized complete block design (5 replicates/treatment and 4 piglets [2 barrows and 2 gilts]/replicate): (1) an antibiotic-free diet (control, CON); (2) CON + 6% wheat bran (WB); (3) CON + 4% sugar beet pulp (SBP). Dietary WB supplementation tended to increase ADG compared with CON from d 1 to 14 ( P = 0.051) and from d 1 to 28 ( P = 0.099). Supplementation of WB increased ( P < 0.05) G:F compared with CON and SBP from d 1 to 14 and from d 1 to 28. Compared with CON, the addition of WB reduced ( P < 0.05) diarrhea rate from d 1 to 14 and tended ( P = 0.054) to reduce diarrhea rate from d 1 to 28. The addition of WB decreased ( P < 0.05) serum diamine oxidase activity on d 14, and up-regulated ( P < 0.05) ileal mRNA levels of occludin on d 28 when compared with CON. Piglets fed WB showed decreased ( P < 0.05) serum interleukin-6 levels compared to those fed SBP and decreased ( P < 0.05) ileal interleukin-8 levels compared to those fed CON and SBP on d 28. Supplementation of WB increased ( P < 0.05) serum levels of immunoglobulin A (IgA), IgG and IgM compared with SBP on d 14, and increased ( P < 0.05) the levels of serum IgA and ileal sIgA compared with CON and SBP on d 28. Piglets fed WB showed an enhanced ( P < 0.05) α-diversity of cecal microbiota than those fed SBP, while piglets fed SBP showed reduced ( P < 0.05) α-diversity of cecal microbiota than those fed CON. Compared with CON, the addition of WB elevated ( P < 0.05) the abundance of Lachnospira and cecal butyric acid level. Piglets fed WB also showed increased ( P < 0.05) abundances of Lachnospira and unclassified_f_Lachnospiraceae compared with those fed SBP. Collectively, the supplementation of WB to antibiotic-free diets improved performance, immune responses, gut barrier function and microbiota compared with the CON and SBP fed piglets. Therefore, supplementing weaned piglets with WB was more effective than SBP.

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Section: Discussionmentioning

confidence: 99%

“…The intestinal microbiota is a complicated ecosystem that has a significant impact on gastrointestinal function, immune function, and health of the host ( Xiong et al., 2019 ). Alpha-diversity is defined as the diversity of organisms within one site or one sample.…”

Section: Discussionmentioning

confidence: 99%

Source of fiber influences growth, immune responses, gut barrier function and microbiota in weaned piglets fed antibiotic-free diets

Shang

Liu

et al. 2021

Animal Nutrition

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“…Weaning stress could induce imperfect and impaired gastrointestinal tract, which is associated with increased permeability, resulting in translocation of pathogens through the intestinal epithelial cells into systemic circulation [8]. DAO, endotoxin and D-lactate are intestinal permeability-related biomarkers and their levels in serum could re ect the intestinal barrier function [35]. In this study, HWP increased serum DAO concentration on day 28 compared with ESBM, indicating that piglets in the ESBM group had better intestinal barrier function.…”

Section: Discussionmentioning

confidence: 58%

“…Weaning process leads to intestinal in ammation and promotes the release of pro-in ammatory cytokines, which can explain the stunted growth performance and intestinal disorders of piglets [36]. Numerous pro-in ammatory cytokines including IL-1β, IL-6, and TNF-α are involved in the regulation of the in ammatory response and their levels could re ect the in ammatory status in the body [35]. Our result showed that ESBM decreased serum levels of IL-1β and IL-6 compared with HWP and FSBM, indicating that ESBM was superior to HWP and FSBM in alleviating weaning stress-induced in ammatory response.…”

Section: Discussionmentioning

confidence: 99%

Different Dietary Protein Sources in Low-Protein Diets Induces Changes in Antioxidant Capacity, Immunity, Fecal Microbiota and Metabolites of Weaned Piglets

Zhang

Long

2021

Preprint

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Background: The inclusion of high-quality proteins and the protein restriction are commonly used in swine production. Our study was conducted to evaluate the effects of hydrolyzed wheat protein (HWP), fermented soybean meal (FSBM), and enzyme-treated soybean meal (ESBM) in low-protein diets on antioxidant capacity, immunity, fecal microbiota and metabolites of weaned piglets.Methods: A total of 144 weaned piglets were randomly assigned to 3 dietary treatments with 6 replications of 8 piglets per pen. The experiment was divided into phase 1 ( days 0-14) and phase 2 (days 15-28). The dietary treatments contained 15.90% HWP, 15.80% FSBM, and 15.10% ESBM in phase 1, and 7.90% HWP, 7.80% FSBM, and 7.50% ESBM in phase 2, respectively.Results: The ADG of piglets in the ESBM group was higher (P < 0.05) than HWP and FSBM during days 1-28. Compared with HWP and FSBM, piglets in ESBM had higher (P < 0.05) serum levels of FRAP and SOD on days 14, as well as higher (P < 0.05) serum FRAP level on days 28. Piglets in ESBM had lower (P < 0.05) serum levels of DAO and IL-1β than HWP on day 28. ESBM significantly increased the abundance of Bacteroidetes, Oscillospiraceae and Christensenellaceae and decreased the Clostridiaceae abundance in the feces compared with HWP and FSBM. The PICRUSt analysis revealed that the number of gene tags involved in valine, leucine and isoleucine degradation and lysine degradation in ESBM were lower (P < 0.05) than HWP and FSBM. Piglets in ESBM had higher (P < 0.05) fecal butyrate content compared with FSBM, and ESBM tended to decrease (P = 0.076) fecal cadaverine level.Conclusions: Overall, ESBM had advantages over HWP and FSBM in improving antioxidant capacity, immunity, and fecal microbiota and metabolites of weaned piglets.

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“…In addition, intestinal in ammation is closely associated with the intestinal barrier. IL-1β, IL-6 and IL-8 were reported to impair intestinal barrier function by rearranging tight junction proteins, while TNF-α signi cantly disrupts the tight junctions between intestinal epithelial cells 48 . Hence, GOS alleviated the colonic in ammation caused by LPS challenge in piglets, which contributed to the maintenance of colonic mucosal barrier function.…”

Section: Discussionmentioning

confidence: 99%

Galacto-Oligosaccharides Improve Barrier Function and Relieve Colonic Inflammation via Modulating Mucosa-Associated Microbiota Composition in Lipopolysaccharides-Challenged Piglets

Gao

Tian

Wang

et al. 2021

Preprint

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BackgroundGalacto-oligosaccharides (GOS) have been shown to modulate the intestinal microbiota of suckling piglets to exert beneficial effects on intestinal function. However, the modulation of intestinal microbiota and intestinal function by GOS in intestinal inflammation injury models has rarely been reported. In this study, we investigated the effects of GOS on the colonic mucosal microbiota composition, barrier function and inflammatory response of lipopolysaccharides-challenged suckling piglets.Resultwe found that the colonic mucosa of LPS-challenged piglets was significantly injured and shedding, while the colonic mucosa of the LPS-GOS group piglets maintained its structure. GOS increased the relative abundance of Lactobacillus (P = 0.058), norank_f__Muribaculaceae and Romboutsia, and decreased the relative abundance of Alloprevotella, Campylobacter and Helicobacter in the colonic mucosa of LPS-challenged suckling piglets. In addition, GOS increased the concentrations of acetate, butyrate and total SCFAs in the colonic digesta of LPS-challenged suckling piglets. Increased SCFAs had beneficial effects on colonic barrier function and inflammatory status, including the enhanced protein expression of Claudin-1 and ZO-1 in the colonic mucosa of LPS-challenged suckling piglets, the decreased concentrations of interleukin 1β (IL-1β), interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α) and cluster of differentiation 14 (CD14), and the decreased relative expression of Toll-like receptor 4 (TLR4) and myeloid differentiation primary response 88 (MyD88).ConclusionsThese results suggested that GOS modulated the colonic mucosa-associated microbiota composition and improved the intestinal function of LPS-challenged suckling piglets.

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Icariin enhances intestinal barrier function by inhibiting NF-κB signaling pathways and modulating gut microbiota in a piglet model

Abstract: This study investigated the effects of icariin on intestinal barrier function and its underlying mechanisms.

Cited by 22 publications

References 61 publications

Source of fiber influences growth, immune responses, gut barrier function and microbiota in weaned piglets fed antibiotic-free diets

Source of fiber influences growth, immune responses, gut barrier function and microbiota in weaned piglets fed antibiotic-free diets

Different Dietary Protein Sources in Low-Protein Diets Induces Changes in Antioxidant Capacity, Immunity, Fecal Microbiota and Metabolites of Weaned Piglets

Galacto-Oligosaccharides Improve Barrier Function and Relieve Colonic Inflammation via Modulating Mucosa-Associated Microbiota Composition in Lipopolysaccharides-Challenged Piglets

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