Ibuprofen and hydrogel-released ibuprofen in the reduction of inflammation-induced migration in melanoma cells

Redpath, Margaret; Marques, C. M. G.; Dibden, Catherine; Waddon, A. J.; Lalla, Ravindra; MacNeil, Sheila

doi:10.1111/j.1365-2133.2009.09220.x

Cited by 32 publications

(30 citation statements)

References 36 publications

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“…When compared to individual migration, collective migration, as in melanomas, presents higher dependence on cell-ECM adhesion [11]. In accordance with this statement, studies have shown that the presence of TNF-increased melanoma cell migration [12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 57%

“…Experimental data used on calibration were based on the work by Redpath et al [15]. Data were obtained from a study using C8161 human cutaneous melanoma, in control media culture and under the influence of pro-inflammatory cytokine TNF-.…”

Section: Calibrationmentioning

confidence: 99%

“…When cutaneous melanoma is surgically removed, an inflammatory environment arises, which can favor local recurrences after surgery [15], and the exact area to be extracted during surgery is still a controversial theme. The area to be extracted is determined with classification, such as the Clark's levels, that establishes a relationship between tumor invasion level and patient's clinical prognosis, and the Breslow thickness, that refined Clark's levels by establishing a relationship between tumor thickness and patient survival prognosis [4].…”

Section: Introductionmentioning

confidence: 99%

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Mathematical Modeling of Melanoma Cell Migration with an Elastic Continuum Model for the Evaluation of the Influence of Tumor Necrosis Factor-Alpha on Migration

Gallinaro

Marques

Azevedo

et al. 2013

Journal of Computational Medicine

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An elastic continuum mathematical model was implemented to study collective C8161 melanoma cell migration during a "scratch wound" assay, in control and under the influence of the proinflammatory cytokine tumour necrosis factor-alpha (TNF-). The model has four constants: force that results from lamellipod formation (F), adhesion constant between cells and extracellular matrix (ECM) (b), cell layer elasticity modulus (k), and growth rate ( ). A nonlinear regression routine was used to obtain the parameters of the model with data from an experiment made with C8161 melanoma cells, with and without TNF-. Coefficient of determination for both situations was 2 = 0.89 and 2 = 0.92, respectively. The parameters values obtained were similar to the ones found in the literature. However, the adhesion constant value decreased with the introduction of TNF-, which is not in accordance with expected since the presence of TNF-is associated with an increased expression of integrins that would promote an enhanced adhesion among cells. The model was used in a study relating to the adhesion constant and cell migration, and the results suggested that cell migration decreases with higher adhesion, which is also not in accordance with expected. These differences would not occur if it was considered that TNF-increases the elasticity modulus of the cell layer.

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Section: Introductionmentioning

confidence: 57%

Section: Calibrationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mathematical Modeling of Melanoma Cell Migration with an Elastic Continuum Model for the Evaluation of the Influence of Tumor Necrosis Factor-Alpha on Migration

Gallinaro

Marques

Azevedo

et al. 2013

Journal of Computational Medicine

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“…In silico results were then compared with the ones collected experimentally in water and in hydrogel environments. The drug investigated in this work is sodium ibuprofene (IP), a member of non-steroidal anti-inflammatory drugs, commonly used for relief from arthritis, fever and as a generic pain reliever [33][34][35] . The chosen hydrogel, specifically developed for central nervous system repair strategies, was obtained by synthesis from statistical block polycondensation between agarose and carbomer 974P (briefly termed as AC, acronymic of their main components), together with cross-linkers 36,37 .…”

Section: Introductionmentioning

confidence: 99%

On the parallelism between the mechanisms behind chromatography and drug delivery: the role of interactions with a stationary phase

Rossi

Castiglione

Salvalaglio

et al. 2017

Phys. Chem. Chem. Phys.

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A huge number of studies and works in drug delivery literature are focused on understanding and modeling transport phenomena, the pivotal point for a good device design. The rationalization of all phenomena involved is fundamental, but several concerns arise leaving many issues unsolved. In order to change point of view we decided to focus our attention on parallelisms between two fields that seem to be very far each other: chromatography and drug release. Taking advantages on the studies conducted by many researchers with chromatographic columns we decided to explain all the phenomena involved in drug delivery considering sodium ibuprofen molecules (IP) as analytes and hydrogel as stationary phase. In particular we considered not only diffusion, but also drug-polymer interactions as adsorption on the stationary phase and drug-drug interactions as analytes aggregation. Hydrogel investigated is a promising formulation made of agarose and carbomer 974p (AC) loaded with IP, a non-steroidal common anti-inflammatory drugs. The self-diffusion coefficient of IP in AC formulations was measured by using an innovative method based on Magic Angle Spinning NMR spectroscopic technique to produce High Resolution (liquid-like) spectra.This method (HR-MAS NMR) is used in combination with pulsed field gradient spin echo (PGSE) liquid-state techniques. The model predictions satisfactorily match with experimental data obtained in water and the gel environment, indicating that the model presented here, despite its simplicity, is able to describe the key phenomena governing the device behavior and could be used to rationalize the experimental activity.!

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“…Um efeito muito similar na redução da migração, também foi observado em células de melanomas, tratadas com ibuprofeno (Redpath et al, 2009). Ensaios de transwell e de monocamada mostraram uma diminuição na migração em câncer de próstata tratada com AINEs (Wynne e Djakiew, 2010).…”

Section: Análise Da Expressão Da Proteínaunclassified

Análise do papel da prostaglandina E2 na proliferação, migração e apoptose na linhagem de glioma humano T98G e o efeito do ácido gama-linolênico e ibuprofeno sobre este prostanóide.

Gomes¹

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Ibuprofen and hydrogel-released ibuprofen in the reduction of inflammation-induced migration in melanoma cells

Abstract: These results demonstrate that TNF-alpha upregulated malignant melanoma migration in vitro and that this could be reduced by ibuprofen both in solution and delivered from a hydrogel. These effects of ibuprofen cannot be attributed simply to induction of apoptosis.

Cited by 32 publications

References 36 publications

Mathematical Modeling of Melanoma Cell Migration with an Elastic Continuum Model for the Evaluation of the Influence of Tumor Necrosis Factor-Alpha on Migration

Mathematical Modeling of Melanoma Cell Migration with an Elastic Continuum Model for the Evaluation of the Influence of Tumor Necrosis Factor-Alpha on Migration

On the parallelism between the mechanisms behind chromatography and drug delivery: the role of interactions with a stationary phase

Análise do papel da prostaglandina E2 na proliferação, migração e apoptose na linhagem de glioma humano T98G e o efeito do ácido gama-linolênico e ibuprofeno sobre este prostanóide.

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