Ibulocydine sensitizes human cancers to radiotherapy by induction of mitochondria-mediated apoptosis

Park, Seok Soon; Kim, Young-Jong; Ju, Eun Jin; Shin, Sang‐Goo; Choi, Jinhyang; Park, Jaesook; Lee, Jae Hee; Lee, Kyung Jin; Park, Jin; Park, Hye Ji; Ko, Eun Jung; Hwang, Jung Jin; Jin, Dong-Hoon; Suh, Nayoung; Cho, Dong‐Hyung; Lee, Jung Shin; Song, Si Yeol; Kim, Byeong Moon; Jeong, Seong‐Yun; Choi, Eun Kyung

doi:10.1016/j.radonc.2014.07.005

Cited by 13 publications

(12 citation statements)

References 19 publications

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“…Radiotherapy is one of the most local treatments against solid tumours inducing cell apoptosis, but is limited by cell resistance and toxicity on peripheral healthy tissues. Therefore, targeting apoptosis represents a potential interest to observe the efficiency of a cancer treatment on tumours (24) and also to quantify the radiation-induced apoptosis on peripheral normal tissues after radiotherapy (25).…”

Section: Introductionmentioning

confidence: 99%

Chemical and in vitro characterizations of a promising bimodal AGuIX probe able to target apoptotic cells for applications in MRI and optical imaging

Dentamaro¹,

Lux²,

Elst³

et al. 2016

Contrast Media & Molecular

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Different studies on AGuIX nanoparticles have been achieved in the biomedical domain, showing that they allow us to combine multimodal and theranostic properties in oncology. The targeting of apoptotic cells presents a wide range of biomedical applications, including the monitoring of antitumoral therapy and the diagnosis of diseases related to this process, such as atherosclerosis, ischemia, chronic inflammation or autoimmune disorders. AGuIX nanoparticles functionalized with a peptide that recognizes apoptotic cells and with organic fluorophores were characterized by several physicochemical and biological methods such as HPLC, relaxometry and photon correlation spectroscopy, which attested to their potential as bimodal tracers detected by optical imaging and MRI. An increase of relaxivity and stability of AGuIX nanoparticles is also observed after their vectorization. The biological efficiency of this novel bimodal probe to target apoptotic cells was evaluated by fluorescence microscopy, relaxometry, MRI and flow cytometry on a lymphoblastic human T-cell line. In vitro cell apoptosis was chemically induced by incubation with camptothecin. Our in vitro experiments showed a significant specificity of vectorized AGuIX nanoparticles for camptothecin-treated cells that suggests their potential efficiency as probes to target apoptosis. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Introductionmentioning

confidence: 99%

Chemical and in vitro characterizations of a promising bimodal AGuIX probe able to target apoptotic cells for applications in MRI and optical imaging

Dentamaro¹,

Lux²,

Elst³

et al. 2016

Contrast Media & Molecular

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show abstract

“…This was further corroborated by significantly decreased cyclinB1 and CDK1 in the combination treatment, thereby inducing G2/M phase arrest. Previous studies have shown that targeting apoptotic pathways is a crucial strategy in antitumor therapy [ 24 ]; Also, the G2/M cell cycle phase is most sensitive to IR [ 25 ]. Based on these results, we postulated that Analgecine in combination with radiotherapy enhanced the antitumor response by activating apoptotic pathways and inducing G2/M phase arrest.…”

Section: Discussionmentioning

confidence: 99%

Analgecine enhances the anti-tumor response of radiotherapy by increasing apoptosis and cell cycle arrest in non-small cell lung cancer

et al. 2017

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We investigated whether Analgecine treatment enhanced the antitumor response of radiotherapy in non-small cell lung cancer (NSCLC) cells. Lewis lung carcinoma (LLC) xenograft mice treated with Analgecine plus irradiation showed reduced tumor growth and increased survival. Tumor cell apoptosis was enhanced by Analgecine, based on TUNEL assays. It also increased plasma levels of pro-inflammatory cytokines (IL-6, IL-12, and IFN-γ) and decreased anti-inflammatory cytokines (TGFβ and IL-10), suggesting an enhanced immune response. Analgecine plus irradiation reduced cell viability and colony formation by A549 NSCLC cells. Analgecine treatments also activated apoptotic signaling with increased levels of pro-apoptotic proteins, including cytochrome c, caspase-3, cleaved caspase-3, caspase-9, p53 and Bax, and decreased Bcl2. Analgecine enhanced G2/M phase arrest in A549 cells by decreasing cyclinB1 and CDK1. These observations demonstrate that Analgecine combined with radiotherapy enhances anti-tumor responses by inducing apoptosis and cell cycle arrest. Moreover, they suggest possible future clinical application of Analgecine for the treatment of NSCLC.

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“…One is an extrinsic pathway mediated by the cell death receptor, and the other is a mitochondriamediated intrinsic pathway. 23 The mitochondriamediated apoptotic pathway causes Cyt C release and cleavage of caspase-3, ultimately resulting in chromatin condensation, DNA fragmentation and formation of apoptotic bodies. 24 Bcl-2 and Bax are major apoptosis-regulating proteins.…”

Section: Discussionmentioning

confidence: 99%

Chemotherapeutic effect of Zerumbone on melanoma cells through mitochondria-mediated pathways

Wang

Han

et al. 2016

Clin Exp Dermatol

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Ibulocydine sensitizes human cancers to radiotherapy by induction of mitochondria-mediated apoptosis

Cited by 13 publications

References 19 publications

Chemical and in vitro characterizations of a promising bimodal AGuIX probe able to target apoptotic cells for applications in MRI and optical imaging

Chemical and in vitro characterizations of a promising bimodal AGuIX probe able to target apoptotic cells for applications in MRI and optical imaging

Analgecine enhances the anti-tumor response of radiotherapy by increasing apoptosis and cell cycle arrest in non-small cell lung cancer

Chemotherapeutic effect of Zerumbone on melanoma cells through mitochondria-mediated pathways

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