Ibudilast reverses the decrease in the synaptic signaling protein phosphatidylethanolamine-binding protein 1 (PEBP1) produced by chronic methamphetamine intake in rats

Charntikov, Sergios; Pittenger, Steven T.; Thapa, Ishwor; Bastola, Dhundy; Bevins, Rick A.; Pendyala, Gurudutt

doi:10.1016/j.drugalcdep.2015.04.012

Cited by 22 publications

(29 citation statements)

References 49 publications

(56 reference statements)

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“…This molecule is downregulated in several neurological conditions such as Alzheimer’s 75 and other memory-related disorders 76 . Its localization at the synapse suggests that it may be a critical regulator of neuronal survival 77 . Downregulation of PEBP1 could be associated with cognitive impairment which has been suggested to occur under exposure to high doses of F 78, 79 , despite this theory still being debated.…”

Section: Discussionmentioning

confidence: 99%

Enteric innervation combined with proteomics for the evaluation of the effects of chronic fluoride exposure on the duodenum of rats

Melo

Perles

Zanoni

et al. 2017

Sci Rep

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Ingested fluoride (F) is absorbed mainly in the small intestine, which is controlled by the Enteric Nervous System (ENS). Although important intestinal symptomatology has been described after excessive F exposure, there have been no studies reporting the effects of F on the ENS. In this study, the effects of chronic F exposure were evaluated on the duodenums of rats through proteomic and morphological analyses. Concentrations of 0, 10, or 50 ppm of F were applied to the drinking water for 30 days. Immunofluorescence techniques were performed in the myenteric plexus of the duodenum to detect HuC/D, neuronal nitric oxide (nNOS), vasoactive intestinal peptide (VIP), calcitonin gene related peptide (CGRP), and substance P (SP). The 50 ppm F group presented a significant decrease in the density of nNOS-IR neurons. Significant morphological alterations were also observed in HUC/D-IR and nNOS-IR neurons; VIP-IR, CGRP-IR, and SP-IR varicosities for both groups (10 and 50 ppm F). Proteomic analysis of the duodenum demonstrated alterations in the expression of several proteins, especially those related to important biological processes, such as protein polymerization, which helps to explain the downregulation of many proteins upon exposure to 50 ppm of F.

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Section: Discussionmentioning

confidence: 99%

Enteric innervation combined with proteomics for the evaluation of the effects of chronic fluoride exposure on the duodenum of rats

Melo

Perles

Zanoni

et al. 2017

Sci Rep

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“…Following colony room acclimation and 90% food restriction, rats were trained to lever press using our lab’s standard protocol (Charntikov et al, 2015; Charntikov et al, 2013; Pittenger et al, 2016). At the start of each session the house light was illuminated and 1 of the 2 levers was insertion into the chamber (randomly selected).…”

Section: Methodsmentioning

confidence: 99%

“…Indwelling jugular catheters were implanted using the standard procedures in our lab (previously described in Charntikov et al, 2015; Charntikov et al, 2013; Pittenger et al, 2016). Rats were anesthetized with a 2:1 ketamine HCl (100 mg/kg; MWI, Boise, ID) plus xylazine HCl (20 mg/kg; MWI, Boise, ID) cocktail (intramuscular; IM).…”

Section: Methodsmentioning

confidence: 99%

“…All rats that displayed motor ataxia within 20 sec were considered patent (cf. Charntikov et al, 2015; Charntikov et al, 2013; Reichel et al, 2008; Pittenger et al, 2016). Rats were excluded from the study if their catheter was not patent.…”

Section: Methodsmentioning

confidence: 99%

“…Levers were inserted individually with the condition that the same lever was not inserted more than 2 times in a row. These procedures were again designed to result in responding on both levers, each having a similar reinforcement history (Charntikov et al, 2015; Charntikov et al, 2013; Pittenger et al, 2016). …”

Section: Methodsmentioning

confidence: 99%

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Nicotine- and cocaine-triggered methamphetamine reinstatement in female and male Sprague-Dawley rats

Pittenger

Chou

Barrett

et al. 2017

Pharmacology Biochemistry and Behavior

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Preclinical studies have demonstrated a return to methamphetamine (meth)-seeking behavior (reinstatement) induced by injections of meth administered by the experimenter (drug-prime). Notably, females tend to be more sensitive to drug-prime; often displaying more reinstatement behavior when compared to males. While meth-primed reinstatement of meth-seeking behavior has been established, little is known about the ability of other drugs of abuse to substitute for meth during drug-primed reinstatement; nicotine and cocaine were the focus of the present work. We also examined if self-administration and/or reinstated meth-seeking behavior was affected by repeated nicotine administration. Male and female Sprague-Dawley rats were trained to self-administer meth during daily sessions. During this self-administration phase, rats were placed into 1 of 2 groups: saline or repeated nicotine exposure. Rats in the repeated nicotine group received nicotine injections 4h after meth self-administration sessions, whereas the remaining rats received saline. Following self-administration was extinction in which meth was no longer available and nicotine was no longer administered. After extinction, rats were tested to determine if 0 (saline), 0.2, and 0.4 mg/kg nicotine reinstated meth-seeking behavior. Three days of re-extinction followed nicotine testing. Finally, rats received reinstatement tests with 0 (saline), 5, and 10 mg/kg cocaine. Nicotine and cocaine reinstated meth-seeking behavior in male and female rats with no difference between the sexes. Repeated nicotine administration potentiated meth reinstatement following the 0.4 mg/kg nicotine-prime. While females may be more sensitive to reinstatement triggered with the original self-administration drug, this effect may not generalize to priming with other drugs of abuse.

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A comprehensive study to delineate the role of an extracellular vesicle‐associated microRNA‐29a in chronic methamphetamine use disorder

Chand

Gowen

Savine

et al. 2021

J of Extracellular Vesicle

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Extracellular vesicles (EVs), which express a repertoire of cargo molecules (cf. proteins, microRNA, lipids, etc.), have been garnering a prominent role in the modulation of several cellular processes. Here, using both non‐human primate and rodent model systems, we provide evidence that brain‐derived EV (BDE) miRNA, miR‐29a‐3p (mir‐29a), is significantly increased during chronic methamphetamine (MA) exposure. Further, miR‐29a levels show significant increase both with drug‐seeking and reinstatement in a rat MA self‐administration model. We also show that EV‐associated miR‐29a is enriched in EV pool comprising of small EVs and exomeres and further plays a critical role in MA‐induced inflammation and synaptodendritic damage. Furthermore, treatment with the anti‐inflammatory drug ibudilast (AV411), which is known to reduce MA relapse, decreased the expression of miR‐29a and subsequently attenuated inflammation and rescued synaptodendritic injury. Finally, using plasma from MUD subjects, we provide translational evidence that EV‐miR29a could potentially serve as a biomarker to detect neuronal damage in humans diagnosed with MA use disorder (MUD). In summary, our work suggests that EV‐associated miR‐29a‐3p plays a crucial role in MUD and might be used as a potential blood‐based biomarker for detecting chronic inflammation and synaptic damage.

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Ibudilast reverses the decrease in the synaptic signaling protein phosphatidylethanolamine-binding protein 1 (PEBP1) produced by chronic methamphetamine intake in rats

Cited by 22 publications

References 49 publications

Enteric innervation combined with proteomics for the evaluation of the effects of chronic fluoride exposure on the duodenum of rats

Enteric innervation combined with proteomics for the evaluation of the effects of chronic fluoride exposure on the duodenum of rats

Nicotine- and cocaine-triggered methamphetamine reinstatement in female and male Sprague-Dawley rats

A comprehensive study to delineate the role of an extracellular vesicle‐associated microRNA‐29a in chronic methamphetamine use disorder

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