Ia‐positive dendritic cells form a tightly meshed network within the human airway epithelium

Holt, Patrick G.; Schon-Hegrad, M A; Phillips, M. James; McMenamin, Paul G.

doi:10.1111/j.1365-2222.1989.tb02752.x

Cited by 174 publications

(94 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This finding might be due to L1 presentation occurring in BALT for the lung or in larynx-associated lymphoid tissue for the trachea preparation that contained a rather high content of B cells after T-cell depletion (40%; data not shown), suggesting that some lymphoid tissue remained associated. A high L1 presentation activity was detected only in the FT or in the CD11c ϩ fraction of the draining TBLN, within 24 h. This probably reflects the migration pathways of DCs that have been primed in the lungs and trachea, where they reside in great numbers (19,21,22). A low-level and transient activity of presentation was also detected in the FT fraction of the sCLN at 48 h. This may result from the migration of DC primed in the nasal mucosa (28), and it suggests that L1 presentation did not occur in the NALT or was below the limit of detection.…”

Section: Discussionmentioning

confidence: 98%

Trachea, Lung, and Tracheobronchial Lymph Nodes Are the Major Sites Where Antigen-Presenting Cells Are Detected after Nasal Vaccination of Mice with Human Papillomavirus Type 16 Virus-Like Particles

Balmelli

Demotz²,

Acha‐Orbea

et al. 2002

J Virol

View full text Add to dashboard Cite

Systemic and mucosal antibodies have been successfully induced following nasal vaccination using live vectors (32,42,44,45), soluble proteins together with cholera toxin (48, 49), or microparticle-delivered antigens (20). Moreover, nasal vaccination has been the most effective method for inducing specific immunity in the genital tract (4,12,13,15,23,34,35,40,43). The inductive sites, where the immune response is mounted after nasal vaccination, remain so far unclear, but their identification is important for the design of efficient protocols for human vaccination. The nasal-associated lymphoid tissue (NALT) is a potential site from which both soluble and particulate antigens can be sampled following nasal administration (reviewed in references 1, 28, and 50). In humans the NALT is absent, but tissue equivalents are formed by the so-called Waldeyer's ring (tonsils, adenoids etc.) (6, 7). Following nasal vaccination, inhaled antigen may also come in contact with other mucosal surfaces, such as the trachea and the lung, where dendritic cells (DC) have been shown to take up antigen and migrate to draining lymph nodes (21, 51). Furthermore, in the lower respiratory tract, the bronchus-associated lymphoid tissue (BALT) (5) and the larynx-associated lymphoid tissue (26) have also been implicated (16). We have been particularly interested in the design of mucosal vaccination strategies against human papillomavirus type 16 (HPV16), which is etiologically linked to more than 50% of cervical cancer (47). Cervical cancer is the second leading cause of cancer deaths in women worldwide, encouraging the development of a vaccine to prevent infection by these viruses. Recently we have shown that nasal vaccination of anesthetized mice with purified HPV16 virus-like-particles (VLPs) induced high levels of HPV16-neutralizing immunoglobulin G and immunoglobulin A in genital secretions (4). Interaction of the antigen with the lung played a predominant role in the efficient induction of these antibodies, although interaction of the VLPs with the NALT was sufficient to induce a mucosal response after parenteral priming. In order to evaluate the respective roles of the upper and lower respiratory tracts in the induction of a specific genital immune response after nasal vaccination, in the present study we localized the sites of uptake and/or presentation of the HPV16 VLP and defined the cell types involved. For this purpose, we constructed a CD4ϩ -T-cell hybridoma (HD9L1) specific for HPV16 L1, the major component of the VLP. HPV16 VLP presentation was examined in different tissues of the upper and lower respiratory tracts and in the corresponding draining LN.

show abstract

Section: Discussionmentioning

confidence: 98%

Trachea, Lung, and Tracheobronchial Lymph Nodes Are the Major Sites Where Antigen-Presenting Cells Are Detected after Nasal Vaccination of Mice with Human Papillomavirus Type 16 Virus-Like Particles

Balmelli

Demotz²,

Acha‐Orbea

et al. 2002

J Virol

View full text Add to dashboard Cite

show abstract

“…The lining airway epithelium of adult experimental animals and humans contains a network of DC which comprise in the range of 400 -800 DC/mm 2 epithelial surface, comparable to the Langerhans cell network of the epidermis (10,11). The airway epithelial DC turn over more rapidly than DC at any other peripheral tissue site with the possible exception of the intestinal wall (12), and accumulating evidence suggests that this continuous and rapid population renewal is a direct result of the intensity of local antigenic stimulation (13).…”

Section: Endritic Cells (Dc)mentioning

confidence: 99%

“…Preparation, fixation, and immunostaining of lung sections and epidermal sheets, and subsequent enumeration of DC and Langerhans cells, was as detailed previously (10,11).…”

Section: Immunohistochemical Analysismentioning

confidence: 99%

Regulation of Dendritic Cell Recruitment into Resting and Inflamed Airway Epithelium: Use of Alternative Chemokine Receptors as a Function of Inducing Stimulus

Stumbles

Strickland

Pimm

et al. 2001

The Journal of Immunology

110

View full text Add to dashboard Cite

Dendritic cells (DC) were purified by flow cytometry from rat tracheal mucosa; they exhibited the phenotypic characteristics of immature DC including high endocytic activity, low CD80/86 expression, and in vitro responsiveness to a broad range of CC chemokines. Daily treatment of adult rats with the selective CCR1 and CCR5 antagonist Met-RANTES reduced baseline numbers of tracheal intraepithelial DC by 50–60%, and pretreatment of animals with Met-RANTES before inhalation of aerosol containing heat-killed bacteria abolished the rapid DC influx into the epithelium that occurred in untreated controls, implicating CCR1 and CCR5 and their ligands in recruitment of immature DC precursors into resting airway tissues and during acute bacterial-induced inflammation. Comparable levels of DC recruitment were observed during airway mucosal Sendai virus infection and after aerosol challenge of sensitized animals with the soluble recall Ag OVA. However, Met-RANTES did not affect these latter responses, indicating the use of alternative chemokine receptors/ligands for DC recruitment, or possibly attraction of different DC subsets, depending on the nature of the eliciting stimulus.

show abstract

“…DC are present in the normal airway epithelium, lung parenchyma, and epithelial surface of the lower respiratory tract (9 -11). The distribution of DC within the epithelium of conducting airways is similar to the Langerhans cell network in the epidermis (12). The presence of DC in human respiratory tract tissues was also noted in the context of several diseases such as histiocytosis X (13), carcinoma (14), and various granulomatous disorders (15,16).…”

mentioning

confidence: 84%

Human Small Cell Lung Carcinoma and Carcinoid Tumor Regulate Dendritic Cell Maturation and Function

et al. 2001

View full text Add to dashboard Cite

The induction of apoptosis in dendritic cells (DC) is a key mechanism by which tumors escape immune recognition and elimination. In fact, a number of studies have showed the correlation between the number of DC within the tumor and the clinical prognosis, suggesting that increased infiltration of tumor tissue by DC was associated with better patient survival and low incidence of metastatic disease. We compared the number of DC and their distribution pattern in human small-cell lung carcinoma and bronchial carcinoid tumor (CT) tissues. Immunohistochemical analysis revealed the presence of cells expressing DC markers CD1a and CD83 in small-cell lung carcinoma tissues and the complete absence of these cells in CT samples. Next, we examined whether human lung tumor cells produce soluble factors that inhibit differentiation of hematopoietic precursors into mature DC. The addition of small-cell lung carcinoma-conditioned medium to CD34 ؉ precursor cell cultures significantly inhibited colony-forming units of DC formation when compared with nontreated control DC cultures. Furthermore, DC generation and differentiation was completely abrogated in CD34 ؉ cell cultures treated with CT-conditioned medium, suggesting that CT-derived factors blocked CD34 ؉ cell differentiation into DC or induced their apoptosis. Finally, flow cytometry analysis of cultured DC confirmed these results. Thus, analysis of our data suggests that human lung tumors produce factors that inhibit DC generation or maturation and may also induce apoptotic death of DC precursors in vitro.

show abstract

Ia‐positive dendritic cells form a tightly meshed network within the human airway epithelium

Cited by 174 publications

References 6 publications

Trachea, Lung, and Tracheobronchial Lymph Nodes Are the Major Sites Where Antigen-Presenting Cells Are Detected after Nasal Vaccination of Mice with Human Papillomavirus Type 16 Virus-Like Particles

Trachea, Lung, and Tracheobronchial Lymph Nodes Are the Major Sites Where Antigen-Presenting Cells Are Detected after Nasal Vaccination of Mice with Human Papillomavirus Type 16 Virus-Like Particles

Regulation of Dendritic Cell Recruitment into Resting and Inflamed Airway Epithelium: Use of Alternative Chemokine Receptors as a Function of Inducing Stimulus

Human Small Cell Lung Carcinoma and Carcinoid Tumor Regulate Dendritic Cell Maturation and Function

Contact Info

Product

Resources

About