α‐Lipoic acid prolongs survival and attenuates acute kidney injury in a rat model of sepsis

Abstract

Acute kidney injury is a frequent and serious complication in patients with severe sepsis. α-Lipoic acid (ALA), a naturally occurring dithiol compound, has been shown to possess anti-inflammatory and anti-oxidative properties. In the present study we investigated whether ALA could attenuate acute kidney injury and improve survival in a rat model of sepsis. Rats were subjected to caecal ligation and puncture (CLP) to induce sepsis. α-Lipoic acid (200 mg/kg) was administered by oral gavage either immediately (early treatment) or 12 h after the surgical procedure (delayed treatment). Both early and delayed ALA treatment effectively prolonged survival, improved pathological damage in kidney tissues and reduced serum blood urea nitrogen and creatinine levels in CLP-induced septic rats. Furthermore, early treatment with ALA markedly inhibited the release of tumour necrosis factor-α, interleukin (IL)-6 and IL-1β into the serum and reduced mRNA and protein expression of inducible nitric oxide synthase and high mobility group box 1 in kidney tissues from CLP-induced rats. Finally, CLP-induced nuclear factor-κB activation in kidney tissues was significantly suppressed by early ALA treatment. Together, the results indicate that ALA is able to reduce mortality and attenuate acute kidney injury associated with sepsis, possibly by anti-inflammatory actions. α-Lipoic acid may be a promising novel agent for the treatment of conditions associated with septic shock.

Alpha-Lipoic Acid Exerts Anti-Inflammatory Effects on Lipopolysaccharide-Stimulated Rat Mesangial Cells via Inhibition of Nuclear Factor Kappa B (NF-κB) Signaling Pathway

“…A recent research from Wongmekiat et al (2013) has demonstrated that ALA mitigates renal injury in rats with obstructive nephropathy [18], suggesting a therapeutic role of ALA in renal diseases. Interestingly, our prior research has demonstrated that ALA can prolong survival and attenuate AKI in a rat model of sepsis, possibly by the anti-inflammatory actions [19]. Nonetheless, the underlying regulatory mechanisms remain unclear and require to be further explored.…”

Protective effect of alpha‐lipoic acid against apical periodontitis‐induced cardiac injury in rats

“…Rats are commonly used in animal experiments of distant organ injury caused by inflammation as they have structural and functional similarities to humans . In this study, rats were chosen to investigate the effect of ALA on AP‐induced cardiac injury.…”

An injectable collagen/poly(γ-glutamic acid) hydrogel as a scaffold of stem cells and α-lipoic acid for enhanced protection against renal dysfunction

“…Because ROS production is a major causative factor of renal dysfunction in cisplatin-induced renal dysfunction, 35 we selected LA as the drug to attenuate the renal dysfunction. [25][26][27][28] As shown in Fig. 7B, the effect of the hydro-gel/LA scaffold on cell viability was similar to that of the hydrogel without LA.…”

“…1). [25][26][27][28] Experimental section Materials Collagen (type I, M w = 250 kDa) was purchased from Bioland (Cheonan, Korea). The γ-PGA (K + form, M w = 500 kDa) was from BioLeaders Corporation (Daejeon, Korea).…”