An injectable collagen/poly(γ-glutamic acid) hydrogel as a scaffold of stem cells and α-lipoic acid for enhanced protection against renal dysfunction

Cho, Sunhee; Noh, Jung‐Ran; Cho, Mi Young; Go, Min‐Jeong; Kim, Yong‐Hoon; Kang, Eun Sung; Lee, Chul‐Ho; Lim, Yong Taik

doi:10.1039/c6bm00711b

Cited by 22 publications

(9 citation statements)

References 34 publications

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“…Copolymers made of conductive poly(3,4-ethylenedioxythiphene) grafted with d , l -PLA (PEDOT- co -PDLLA) were synthesized and tested with embryonic stem cells (ESCs) to obtain biodegradable biomaterials with electric properties . Many other biomaterials based on poly(α-hydroxy) esters and derived copolymers have been synthesized and investigated, taking advantage of their approved use in clinical fields and their processability by different fabrication strategies. − Some examples of synthetic polymers employed for stem cell encapsulation are reported in Table . …”

Section: Synthetic Strategies To Mimic Extracellular Matricesmentioning

confidence: 99%

3D Extracellular Matrix Mimics: Fundamental Concepts and Role of Materials Chemistry to Influence Stem Cell Fate

et al. 2020

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Synthetic 3D extracellular matrices (ECMs) find application in cell studies, regenerative medicine, and drug discovery. While cells cultured in a monolayer may exhibit unnatural behavior and develop very different phenotypes and genotypes than in vivo, great efforts in materials chemistry have been devoted to reproducing in vitro behavior in in vivo cell microenvironments. This requires fine-tuning the biochemical and structural actors in synthetic ECMs. This review will present the fundamentals of the ECM, cover the chemical and structural features of the scaffolds used to generate ECM mimics, discuss the nature of the signaling biomolecules required and exploited to generate bioresponsive cell microenvironments able to induce a specific cell fate, and highlight the synthetic strategies involved in creating functional 3D ECM mimics.

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Section: Synthetic Strategies To Mimic Extracellular Matricesmentioning

confidence: 99%

3D Extracellular Matrix Mimics: Fundamental Concepts and Role of Materials Chemistry to Influence Stem Cell Fate

et al. 2020

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“…Cell activity was quantitatively analyzed with CCK-8 assay [23]. A total of 20 μL cell suspensions at a density of 5×10 5 cells ml −1 were seeded into lung scaffolds in a 24-well plate.…”

Section: Cell Viabilitymentioning

confidence: 99%

A novel tissue-engineered 3D tumor model for anti-cancer drug discovery

Yang

et al. 2018

Biofabrication

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Cancer biology and drug discovery are heavily dependent on conventional 2D cell culture systems. However, a 2D culture is significantly limited by its ability to reflect 'true biology' of tumor in vivo. Three-dimensional (3D) in vitro cell culture models have been introduced to aid cancer drug discovery by better modeling the tumor microenvironment. Here, decellularized lung scaffolds cultured with MCF-7 cancer cells were bioengineered as a platform to study tumor development and anti-cancer drug evaluation. Excellent cell compatibility of decellularized lung scaffolds promoted cell growth and proliferation. Multicellular tumor cell spheroids (tumoriods) were formed and enlarged exclusively in decellularized lung scaffolds over time. The expression of breast cancer biomarkers (BRCA1 and HER2) in MCF-7 cells significantly increased in the lung matrix compared to those cultured in 2D systems. Insufficient oxygen and nutrient diffusion into the internal surface of lung scaffolds resulted in intracellular hypoxia, quantified by a significant upregulation of HIF-1α protein expression compared to that of cell monolayers. Higher survival rates after exposure to 5-FU were observed in lung scaffolds (52.04%) compared to that in 2D systems (18.39%) on day 3 of culture. Overall, this new breast tumor system provides a promising platform to study breast cancer progression and develop new targeted therapeutic strategies.

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“…Typically, these hydrogels comprised naturally derived and highly biocompatibility polymeric materials. Examples of promising hydrogels for cell therapy of CKD included the following: HA/collagen cross-linked with polyethylene glycol diacrylate (PEG-DA) and loaded with bioactive factors such as stromal cell-derived factor (SDF)-1 [141][142][143]; collagen/poly γ-glutamic acid (γ-PGA) loaded with antioxidant α-lipoic acid [144]; and decellularised bovine pericardium [145]. In rodent models of adriamycin-induced nephrotoxicity or bilateral ischemia [142], LPS-induced endotoxemia [141,143], and cisplatin-induced renal dysfunction [144], hydrogels prolonged the paracrine effects and improved retention of co-transplanted stem cells, protecting them from the inflammatory and pathological tissue microenvironment.…”

Section: Hydrogel Carriers For Ckdmentioning

confidence: 99%

Hydrogel and nanoparticle carriers for kidney disease therapy: trends and recent advancements

Liu

Tai

et al. 2022

Prog. Biomed. Eng.

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Achieving local therapeutic agent concentration in the kidneys through traditional systemic administration routes have associated concerns with off-target drug effects and toxicity. Additionally, kidney diseases are often accompanied by co-morbidities in other major organs, which negatively impacts drug metabolism and clearance. To circumvent these issues, kidney-specific targeting of therapeutics aims to achieve the delivery of controlled doses of therapeutic agents, such as drugs, nucleic acids, peptides, or proteins, to kidney tissues in a safe and efficient manner. Current carrier material approaches implement macromolecular and polyplex hydrogel constructs, prodrug strategies, and nanoparticle-based delivery technologies. In the context of multidisciplinary and cross-discipline innovations, the medical and bioengineering research fields have facilitated the rapid development of kidney-targeted therapies and carrier materials. In this review, we summarize the current trends and recent advancements made in the development of carrier materials for kidney disease targeted therapies, specifically hydrogel and nanoparticle-based strategies for acute kidney disease, chronic kidney disease, and renal cell carcinoma. Additionally, we discuss the current limitations in carrier materials and their delivery mechanisms.

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An injectable collagen/poly(γ-glutamic acid) hydrogel as a scaffold of stem cells and α-lipoic acid for enhanced protection against renal dysfunction

Cited by 22 publications

References 34 publications

3D Extracellular Matrix Mimics: Fundamental Concepts and Role of Materials Chemistry to Influence Stem Cell Fate

3D Extracellular Matrix Mimics: Fundamental Concepts and Role of Materials Chemistry to Influence Stem Cell Fate

A novel tissue-engineered 3D tumor model for anti-cancer drug discovery

Hydrogel and nanoparticle carriers for kidney disease therapy: trends and recent advancements

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