<i>Xanthium strumarium</i>L. Extracts Produce DNA Damage Mediated by Cytotoxicity in<i>In Vitro</i>Assays but Does Not Induce Micronucleus in Mice

Ferrer, Janet Piloto; Cozzi, Renata; Cornetta, Tommaso; Stano, Pasquale; Fiore, Mario; Degrassi, Francesca; Salvia, R. De; Remigio, Antonia; Francisco, Marbelis; Rutiaga-Quiñones, Olga Miriam; Valdivia, Dayana; González, Maria L.; Pérez, Carlos L; Sánchez-Lamar, Ángel

doi:10.1155/2014/575197

Cited by 16 publications

(8 citation statements)

References 24 publications

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“…Our study demonstrates that XFC selectively induces mitotic arrest in ES-2 and SKOV-3 cells, leading to decreased cell growth and viability in a dose- and time-dependent manner (Figure 4). XFC also inhibited normal mitotic progression by interfering with the metaphase to anaphase transition, consistent with previous data showing an antitubulin action of the extract [8, 17]. Our data, showing an impairment in anaphase entrance, demonstrates that XFC interferes with the normal function of the mitotic spindle, effects similar to those displayed by other well-known antimitotic drugs [36].…”

Section: Discussionsupporting

confidence: 91%

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Targeting Ovarian Cancer Cell Cytotoxic Drug Resistance Phenotype with Xanthium strumarium L. Extract

Fernandez

Charfi

Piloto-Ferrer³

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Emerging drugs aim at targeting the genomic integrity and replication machinery in ovarian cancer. While the antiproliferative activity of Xanthium strumarium L. extract (XFC), a traditional herbal medicine, is believed to alter the mitotic apparatus of Chinese hamster ovary epithelial cells, its capacity to target and overcome the chemoresistance phenotype in ovarian cancer is unknown. Among the cancer cell lines tested, we found that the best proliferation inhibitory effect for XFC was against ovarian cancer cells and ranged from 30 to 35 μg/mL. XFC efficiently targeted both the cytotoxic drug chemoresistance phenotype of SKOV-3 cells and of the chemosensitive ES-2 cells. Early apoptosis and late apoptosis were effectively induced by XFC extract in ES-2 cells, whereas late apoptosis and necrosis events were triggered in SKOV-3 cells. Cell cycling regulation was trapped by XFC extract in the G2/M phase in both the ES-2 and SKOV-3 cell models. This effect was, in part, attributable to increased dose-dependent tubulin polymerization, which was increased in SKOV-3 cells. Whereas XFC extract triggered poly (ADP-Ribose) polymerase (PARP) cleavage in both ES-2 and SKOV-3 cells, it only lowered Nrf2 in ES-2 cells and phosphorylated Akt levels in SKOV-3 cells. Interestingly, cell cycling regulators Cdk4, Cyclin D3, and p27 were all decreased in SKOV-3 cells. XFC extracts were effective in inhibiting in vitro migration in both ovarian cancer cell models. Our data support the potential anticancer targeting of chemoresistant human ovarian cancer cells phenotype by XFC extract.

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Section: Discussionsupporting

confidence: 91%

“…A voucher specimen with number ROIG 4594 was deposited at the herbarium of this institution. Plant parts (500 g) were extracted with 70% ethanol as described elsewhere [17]. A fluid extract was prepared from the dried material by hydroalcoholic extraction in using four rounds of percolation.…”

Section: Methodsmentioning

confidence: 99%

Targeting Ovarian Cancer Cell Cytotoxic Drug Resistance Phenotype with Xanthium strumarium L. Extract

Fernandez

Charfi

Piloto-Ferrer³

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…Similar approach has been used to investigate cytotoxic and antimitotic potential of many plants. Evaluation of in vitro cytotoxicity which cause DNA damage in mice was studied by using extract of Xanthium strumarium L. [34]. Potential genotoxic activity in Pterocaulon polystachyum through A. cepa test detected an antimitosis property related to increasing concentrations of aqueous extracts [35].…”

Section: Discussionmentioning

confidence: 99%

In vitro antimitotic and cytotoxic potential of plant extracts: a comparative study of Mucuna pruriens, Asteracantha longifolia and Sphaeranthus indicus

Gupta

Patel

2020

Futur J Pharm Sci

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Background Plants have been used in alternative and traditional medicines for the cure of different types of diseases since ancient time. Secondary metabolites from natural sources play a crucial role in the treatment of various ailments. The present study carried out to investigate the phytochemical, antimitotic and cytotoxic activity of methanolic (95%) extracts of Mucuna pruriens seeds, Asteracantha longifolia seeds and Sphaeranthus indicus stems. Result Phytochemical analysis was performed using qualitative test to confirm the presence of phytochemical such as flavonoids, terpenoids, amino acids, cardiac glycosides, saponins, steroids, tannins, phenols and carbohydrates. The antimitotic activity was screened by using Allium cepa root meristematic cells. Methotrexate (0.1 mg/mL) was used as a standard. The data was analyzed by using software GraphPad Prism, Version 6.0 (GraphPad Software Inc., San Diego, CA) with one-way ANOVA. A statistical difference of p < 0.05 was considered significant in all cases. p value of M. pruriens seeds, A. longifolia seeds and S. indicus stems calculated p = 0.0001 for all plant extracts. Cytotoxic potential of all three plant extracts have been studied on breast cancer cell line MCF7 and lung cancer cell line A549. M. pruriens showed mild cytotoxicity with IC50 values 36.74 μg/mL on MCF7 and 39.42 μg/mL on A549 cell line. A. longifolia showed better activity on MCF7 with IC50 of 12.32 μg/mL and the S. indicus showed the least activity on MCF7 with IC50 of 185.56 μg/mL. The A. longifolia showed better activity on A549 with IC50 of 16.53 μg/mL. Conclusion A. longifolia has significant amount of nearly all phytochemicals as compared to other two plant extracts. It is found that all three plant extracts showed antimitotic activity having p value less than 0.05. The cytotoxicity assay revealed that all plant extracts displayed inhibition of MCF7 and A549 cells lines. A. longifolia showed better activity against MCF7 while M. pruriens possessed mild cytotoxic effect against both MCF7 and A549 cell lines.

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“…In 2013, Yu et al indicated that WEX at concentrations 100 μg/mL can inhibit growth of HK-2 cells [151]. Moreover, HEXA (25–100 μg/mL) also causes in vitro DNA damage at cytotoxic concentrations through sister chromatid exchanges, chromosome aberrations, and comet assay, meanwhile, it also shows significant reduction in CHO cell viability [152]. In 2016, Su et al compared the cytotoxicities of the components with different polarities, and study indicated that EAFEEX (IC 50 = 231.1 μg/mL) was the most toxic part [153].…”

Section: Toxicitymentioning

confidence: 99%

Traditional Uses, Botany, Phytochemistry, Pharmacology, Pharmacokinetics and Toxicology of Xanthium strumarium L.: A Review

Fan

Peng

et al. 2019

Molecules

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Xanthium strumarium L. (Asteraceae) is a common and well-known traditional Chinese herbal medicine usually named Cang-Er-Zi, and has been used for thousands of years in China. The purpose of this paper is to summarize the progress of modern research, and provide a systematic review on the traditional usages, botany, phytochemistry, pharmacology, pharmacokinetics, and toxicology of the X. strumarium. Moreover, an in-depth discussion of some valuable issues and possible development for future research on this plant is also given. X. strumarium, as a traditional herbal medicine, has been extensively applied to treat many diseases, such as rhinitis, nasal sinusitis, headache, gastric ulcer, urticaria, rheumatism bacterial, fungal infections and arthritis. Up to now, more than 170 chemical constituents have been isolated and identified from X. strumarium, including sesquiterpenoids, phenylpropenoids, lignanoids, coumarins, steroids, glycosides, flavonoids, thiazides, anthraquinones, naphthoquinones and other compounds. Modern research shows that the extracts and compounds from X. strumarium possess wide-ranging pharmacological effects, including anti- allergic rhinitis (AR) effects, anti-tumor effects, anti-inflammatory and analgesic effects, insecticide and antiparasitic effects, antioxidant effects, antibacterial and antifungal effects, antidiabetic effects, antilipidemic effects and antiviral effects. However, further research should focus on investigating bioactive compounds and demonstrate the mechanism of its detoxification, and more reasonable quality control standards for X. strumarium should also be established.

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Xanthium strumariumL. Extracts Produce DNA Damage Mediated by Cytotoxicity inIn VitroAssays but Does Not Induce Micronucleus in Mice

Cited by 16 publications

References 24 publications

Targeting Ovarian Cancer Cell Cytotoxic Drug Resistance Phenotype with Xanthium strumarium L. Extract

Targeting Ovarian Cancer Cell Cytotoxic Drug Resistance Phenotype with Xanthium strumarium L. Extract

In vitro antimitotic and cytotoxic potential of plant extracts: a comparative study of Mucuna pruriens, Asteracantha longifolia and Sphaeranthus indicus

Traditional Uses, Botany, Phytochemistry, Pharmacology, Pharmacokinetics and Toxicology of Xanthium strumarium L.: A Review

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