Viscum album L. homogenizer‐assisted and ultrasound‐assisted extracts as potential sources of bioactive compounds

Abstract

Viscum album L. (Mistletoe) is one of the most famous plants in many countries utilized for several purposes. The current study aimed to describe chemical profiles and biological activities of homogenizer‐assisted extract (HAE) and ultrasound‐assisted extract (UAE)) of V. album parts (leaf, fruits, and seeds). Antioxidant (radical scavenging, reducing power, metal chelation, and phosphomolybdenum assays) and enzyme inhibitory properties (cholinesterases, amylase, glucosidase, and tyrosinase) were selected for biological evaluation. Chemical profiles were studied by HPLC‐MS/MS and 32 compounds were identified in the extracts; caffeoylquinic acids and its derivatives, dimethylated flavonoids were the most significant compounds. Generally, the leaf extracts exhibited the best antioxidant and enzyme inhibitory effects in our tests. Multivariate analysis was performed to obtain more information for these data, then strong correlations between total bioactive compounds and tested parameters were observed. The present findings encourage us to further investigate V. album as a potential candidate for pharmaceutical and nutraceutical applications. Practical applications Viscum album L. commonly called European mistletoe is a woody perennial shrub growing on coniferous trees with lathery leaves, small flowers, and white berries. It belongs to the Santalaceae R. Br. family from Europe and western/southern Asia. Traditional medicine recognizes mistletoe as a folk remedy to manage inflammation, hypertension, ulcers, and other diseases due to the presence of different bioactive compounds, among them mistletoe lectins and viscotoxins. Recent studies documented the possible therapeutic applications of Viscum extracts in association with cancer's therapy leading to improvements in health and patient's quality of life. Thus, this work gives novel data regarding the phytochemical characterizations and antioxidant/enzymatic inhibitor activities of different types of extracts from seeds, leaves, and fruits of Viscum L. obtained by homogenizer‐assisted and ultrasound‐assisted techniques, in order to increase the data set of potential applications in medicine.

Dynamics of Polyphenol Biosynthesis by Calli Cultures, Suspension Cultures and Wild Specimens of the Medicinal Plant Ligaria cuneifolia (Ruiz & Pav.) Tiegh. (Loranthaceae). Analysis of Their Biological Activity

“…These in vitro results correlate with the in vivo and ex vivo experimental designs previously performed in L. cuneifolia [11]. In accordance with our findings, a linear correlation between the total phenolic level and antioxidant properties was described in V. album, which was attributed to the presence of phenolic compounds in earlier studies [28,60].…”

“…QE-3-O-glycosides and galloyl glycosides were also present at lower values. Our results are in agreement with those found in V. album, in which leaf extracts showed higher concentrations of total phenolics and flavonoids compared to fruit and seed extracts [28]. Similarities in polyphenolic profile but quantitative differences among growing seasons were also reported in C. palirus leaves.…”

Impact of Harvest Conditions and Host Tree Species on Chemical Composition and Antioxidant Activity of Extracts from Viscum album L.

“…As shown by the available literature data, the analysis of phenolic acids in mistletoe from different the host trees has been an object of scientific interest. Qualitative studies of phenolic acids were carried out by Krzaczek (1977) [ 26 ] and quantitative studies were conducted by Łuczkiewicz et al (2001) [ 28 ], Vicas et al (2011) [ 33 ], and Stefanucci et al (2020) [ 34 ]. Flavonoid aglycones were studied by Haas et al (2003) [ 35 ], Vicas et al (2011) [ 33 ], and Stefanucci et al (2020) [ 34 ].…”

“…Qualitative studies of phenolic acids were carried out by Krzaczek (1977) [ 26 ] and quantitative studies were conducted by Łuczkiewicz et al (2001) [ 28 ], Vicas et al (2011) [ 33 ], and Stefanucci et al (2020) [ 34 ]. Flavonoid aglycones were studied by Haas et al (2003) [ 35 ], Vicas et al (2011) [ 33 ], and Stefanucci et al (2020) [ 34 ]. The chemical composition of mistletoe was the subject of some publications, but these studies in most cases were focused on Viscum species without specifying the host trees from which the plant material was collected.…”

Polysaccharides of Scrophularia ningpoensis Hemsl.: Extraction, Antioxidant, and Anti‐Inflammatory Evaluation

“…This method is widely used, simple to operate, and associated with high extraction efficiency [ 15 , 16 ]. More importantly, UAE has potential advantages of protecting the structure and biological activity of active components [ 17 – 19 ].…”