<i>Vibrio parahaemolyticus</i>
            Serovar O3:K6 as Cause of Unusually High Incidence of Food-Borne Disease Outbreaks in Taiwan from 1996 to 1999

Chiou, Chien-Shun; Hsu, Shih-Yuan; Chiu, Shiou-Ing; Wang, Tien-Kuei; Chao, Cheng-Shun

doi:10.1128/jcm.38.12.4621-4625.2000

Cited by 110 publications

(41 citation statements)

References 14 publications

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“…However, the presence of a clone with the characteristics of a pandemic clone in the two outbreaks and in various isolated cases of gastroenteritis, suggests that this is disseminated in the region. In fact, such situation finds support in the observations made with the serotype O3:K6 in various countries of the Far East (Okuda et al 1997;Chiou et al 2000;Matsumoto et al 2000;Wong et al , 2007, which is why the clone has been considered pandemic.…”

Section: Discussionmentioning

confidence: 78%

Vibrio parahaemolyticusserovar O3:K6 gastroenteritis in northeast Brazil

Leal¹,

Silva²,

Cavalcanti

et al. 2008

Journal of Applied Microbiology

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Aims: To examine the virulence factors and the genetic relationship isolates of the serogroup O3 of Vibrio parahaemolyticus in outbreaks of diarrhoea in the northeast region of Brazil. Methods and Results: Eighteen samples of the O3:K6 and O3:KUT serotypes of V. parahaemolyticus were analysed by multiplex polymerase chain reaction (m‐PCR) for detection of the tl, tdh and trh genes, by random‐amplified polymorphic DNA (RAPD) using two primers, and by amplification of the rDNA 16S–23S region. The gene tl was amplified in all the samples, tdh in 16 while trh in none; amplification of rDNA 16S–23S generated only one profile; each RAPD primer produced two amplification patterns allowing grouping two tdh– Kanagawa‐negative isolates. Conclusions: V. parahaemolyticus with characteristics of the pandemic clone appears to be widely disseminated in the studied region. Because of the genetic uniformity of the isolates, elucidation of outbreaks or tracking the source of contamination by the present molecular techniques seems useless. Significance and Impact of the Study: Detection of V. parahaemolyticus with virulence potential of pandemic clone from two outbreaks and from several isolated gastroenteritis cases points out the need for inclusion of this micro‐organism in the Brazilian routine monitoring of the diarrhoeas for elucidation of their aetiology.

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Section: Discussionmentioning

confidence: 78%

Vibrio parahaemolyticusserovar O3:K6 gastroenteritis in northeast Brazil

Leal¹,

Silva²,

Cavalcanti

et al. 2008

Journal of Applied Microbiology

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“…S. pyogenes and S. aureus were grown in TSBY broth or TSBY broth agar (MDBio, Inc., Taipei, Taiwan) at 37°C. V. parahaemolyticus (strain 15427, serovar O3:K6) was a clinical isolate obtained from the Center for Disease Control in Taiwan (8). The strains were maintained and grown at 37°C in tryptic soy broth (Difco) supplemented with 3% NaCl.…”

Section: Methodsmentioning

confidence: 99%

Visible-Light-Induced Bactericidal Activity of a Nitrogen-Doped Titanium Photocatalyst against Human Pathogens

Wong

Chu²,

Sun

et al. 2006

Appl Environ Microbiol

199

135

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The antibacterial activity of photocatalytic titanium dioxide (TiO 2 ) substrates is induced primarily by UV light irradiation. Recently, nitrogen-and carbon-doped TiO 2 substrates were shown to exhibit photocatalytic activities under visible-light illumination. Their antibacterial activity, however, remains to be quantified. In this study, we demonstrated that nitrogen-doped TiO 2 substrates have superior visible-light-induced bactericidal activity against Escherichia coli compared to pure TiO 2 and carbon-doped TiO 2 substrates. We also found that protein-and light-absorbing contaminants partially reduce the bactericidal activity of nitrogen-doped TiO 2 substrates due to their light-shielding effects. In the pathogen-killing experiment, a significantly higher proportion of all tested pathogens, including Shigella flexneri, Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, Streptococcus pyogenes, and Acinetobacter baumannii, were killed by visible-light-illuminated nitrogen-doped TiO 2 substrates than by pure TiO 2 substrates. These findings suggest that nitrogen-doped TiO 2 has potential application in the development of alternative disinfectants for environmental and medical usages.

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“…Vibrio parahaemolyticus is considered to be pathogenic for both humans and marine life. In humans, this organism has been reported in several countries and continents as a major cause of seafood-caused illnesses after the consumption of raw or inadequately or improperly cooked seafood (Chiou et al 2000;Daniels et al 2000;Vuddhakul et al 2000;Alam et al 2002;McLaughlin et al 2005). In aquatic animals, V. parahaemolyticus can cause serious diseases in fish, shellfish and penaeid shrimp leading to significant losses in aquaculture industries (Su and Liu 2007).…”

Section: Introductionmentioning

confidence: 99%

The development of loop-mediated isothermal amplification combined with lateral flow dipstick for detection of Vibrio parahaemolyticus

Prompamorn

Sithigorngul

Rukpratanporn

et al. 2011

Letters in Applied Microbiology

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Aims: The current study was aimed to develop a loop‐mediated isothermal amplification (LAMP) combined with amplicon detection by chromatographic lateral flow dipstick (LFD) assay for rapid and specific detection of Vibrio parahaemolyticus. Methods and Results: Biotinylated LAMP amplicons were produced by a set of four designed primers that recognized specifically the V. parahaemolyticus thermolabile haemolysin (tlh) gene followed by hybridization with an FITC‐labelled probe and LFD detection. The optimized time and temperature conditions for the LAMP assay were 90 min at 65°C. The LAMP–LFD method accurately identified 28 isolates of V. parahaemolyticus but did not detect 24 non‐parahaemolyticus Vibrio isolates and 35 non‐Vibrio bacterial isolates. The sensitivity of LAMP–LFD for V. parahaemolyticus detection in pure cultures was 120 CFU ml−1. In the case of spiked shrimp samples without enrichment, the detection limit for V. parahaemolyticus was 1·8 × 103 CFU g−1 or equivalent to 3 CFU per reaction while that of conventional PCR was 30 CFU per reaction. Conclusions: The established LAMP–LFD assay targeting tlh gene was specific, rapid and sensitive for identification of V. parahaemolyticus. Significance and Impact of the Study: The developed LAMP–LFD assay provided a valuable tool for detection of V. parahaemolyticus and can be used effectively for identification of V. parahaemolyticus in contaminated food sample.

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Vibrio parahaemolyticus Serovar O3:K6 as Cause of Unusually High Incidence of Food-Borne Disease Outbreaks in Taiwan from 1996 to 1999

Cited by 110 publications

References 14 publications

Vibrio parahaemolyticusserovar O3:K6 gastroenteritis in northeast Brazil

Vibrio parahaemolyticusserovar O3:K6 gastroenteritis in northeast Brazil

Visible-Light-Induced Bactericidal Activity of a Nitrogen-Doped Titanium Photocatalyst against Human Pathogens

The development of loop-mediated isothermal amplification combined with lateral flow dipstick for detection of Vibrio parahaemolyticus

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