<i>Vibrio metschnikovii</i>Pneumonia

Wallet, Frédéric; Tachon, M.; Nseir, Saad; Courcol, René; Roussel-Delvallez, M.

doi:10.3201/eid1110.050177

Cited by 22 publications

(14 citation statements)

References 9 publications

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“…The pathogenic bacteria Vibrios produce various virulent factors, including adhesin, polysaccharide, enterotoxin, cytotoxin, hemolysin and proteases (1). In particular, extracellular proteases produced by Vibrio vulnificus (V. vulnificus), V. mimicus, and V. cholerae have been known to play various roles in bacterial infection.…”

Section: Introductionmentioning

confidence: 99%

“…metschnikovii is a gram-negative alkalophilic human pathogenic bacterium (1,7,8). This facultative bacterium is often found in natural aquatic environments such as seawater (9).…”

Section: Introductionmentioning

confidence: 99%

“…This facultative bacterium is often found in natural aquatic environments such as seawater (9). In addition, the bacterium has been known as one of causative agents that can induce dyspneic symptoms (10), diarrhea (11,12), wound infection (13), pneumonia (1) and leg ulcer (9). There have been brief reports on alkaline proteases (AprB, AprJ1, VapT and VapK) from a few V. metschnikovii strains.…”

Section: Introductionmentioning

confidence: 99%

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Purification and characterization of a novel alkaline serine protease secreted by Vibrio metschnikovii

Park

Park²,

Park³

et al. 2011

Int J Mol Med

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Abstract.A novel extracellular alkaline serine protease secreted by Vibrio metschnikovii (V. metschnikovii) ATCC700040 cells was purified by three chromatographic steps and characterized in terms of enzymatic kinetics and substrate specificity. The purified enzyme (named AKP-Vm) was composed of a single polypeptide with an apparent molecular weight of 50 kDa on 12% SDS-polyacrylamide gel in the presence of CuCl 2 . The optimal temperature and the pH for the enzyme were found to be 37˚C and 9.5, respectively. However, the enzyme activity was inhibited by inhibitors such as PMSF and aprotinin. AKP-Vm could hydrolyze a peptide bond at the carboxyl side of the arginine residue, as revealed by its amidolytic activity toward a chromogenic substrate, Boc-Val-Pro-Arg-pNA. . AKP-Vm protease could cleave various blood coagulation-associated proteins, including fibrinogen, prothrombin and thrombin. In particular, the enzyme showed powerful fibrinogenolytic and fibrinolytic activities, as it could cleave all major chains of fibrinogen and also digest cross-linked fibrin. The results obtained suggest that AKP-Vm is a novel alkaline serine protease that can actively cleave fibrinogen and cross-linked fibrin.

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Section: Introductionmentioning

confidence: 99%

“…metschnikovii is a gram-negative alkalophilic human pathogenic bacterium (1,7,8). This facultative bacterium is often found in natural aquatic environments such as seawater (9).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Purification and characterization of a novel alkaline serine protease secreted by Vibrio metschnikovii

Park

Park²,

Park³

et al. 2011

Int J Mol Med

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“…Several Vibrio species are also important opportunistic human pathogens. The best known of these are Vibrio cholerae (20)(21)(22), Vibrio parahaemolyticus (23,24), and Vibrio vulnificus (15,25), but Vibrio cincinnatiensis (26), Vibrio fluvialis (27)(28)(29), Vibrio furnissii (30), Vibrio metschnikovii (31)(32)(33), and Vibrio mimicus (34)(35)(36) can also cause infections in human hosts. These organisms are obviously of great interest, as are additional Vibrio species that have been shown to carry an array of virulence-related genes (1).…”

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Optimization of Multilocus Sequence Analysis for Identification of Species in the Genus Vibrio

Gabriel

Matsui

Friedman

et al. 2014

Appl Environ Microbiol

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Multilocus sequence analysis (MLSA) is an important method for identification of taxa that are not well differentiated by 16S rRNA gene sequences alone. In this procedure, concatenated sequences of selected genes are constructed and then analyzed. The effects that the number and the order of genes used in MLSA have on reconstruction of phylogenetic relationships were examined. The recA, rpoA, gapA, 16S rRNA gene, gyrB, and ftsZ sequences from 56 species of the genus Vibrio were used to construct molecular phylogenies, and these were evaluated individually and using various gene combinations. Phylogenies from two-gene sequences employing recA and rpoA in both possible gene orders were different. The addition of the gapA gene sequence, producing all six possible concatenated sequences, reduced the differences in phylogenies to degrees of statistical (bootstrap) support for some nodes. The overall statistical support for the phylogenetic tree, assayed on the basis of a reliability score (calculated from the number of nodes having bootstrap values of >80 divided by the total number of nodes) increased with increasing numbers of genes used, up to a maximum of four. No further improvement was observed from addition of the fifth gene sequence (ftsZ), and addition of the sixth gene (gyrB) resulted in lower proportions of strongly supported nodes. Reductions in the numbers of strongly supported nodes were also observed when maximum parsimony was employed for tree construction. Use of a small number of gene sequences in MLSA resulted in accurate identification of Vibrio species.

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“…Only four cases of bacteremia in adults [4][5][6] and one neonatal sepsis 9 , a postoperative wound infection in a leg 7 and one case of pneumonia 10 have been reported. It has also been isolated from the urine of two patients 3 , from an ulcer in the foot 3 , from the feces of five children suffering from watery diarrhea 1 and in pure culture from the feces of a patient with diarrhea 8 .…”

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confidence: 99%

Vibrio metschnikovii from a human infected leg ulcer

Martín

Garaizabal

Sánchez

et al. 2008

Rev. Inst. Med. trop. S. Paulo

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Dear Sir:Vibrio metschnikovii is a gram-negative bacillus that can be found in various aquatic habitats including fresh, brackish and marine waters and sewage, as well as shellfish, birds, poultry and domestic ungulates 2,3,7 . The first case of infection in humans was published in 1981 6 and very few cases have been reported since then. We did not find any reference to the isolation of V. metschnikovii in Spain. We report a case where V. metschnikovii was isolated from an infected ulcer in the leg.The patient was a 49-year-old woman from Uruguay who had been living in our country for five months, with a 7-year history of fibromyalgia and frequently infected leg ulcers. She came to our hospital´s emergency service suffering from painful ulcers in both legs, which carried a chronic lymphoedema background. Swabs with exudates from ulcers were sent to the laboratory for microbiological study and a regular cure was prescribed. Mixed flora -consisting of multiresistant bacterial organisms -was isolated from both legs. In addition, V. metschnikovii was isolated from the left lower limb. This microorganism formed grey colonies (diameter: 2-3 mm) with complete hemolysis on blood agar after 24 hours of incubation at 36 °C. It formed lactose positive colonies on MacConkey agar and yellowish ones on thiosulfate-citrate-bile salts-sucrose agar. Gram staining of the colonies showed curved gram-negative rods. It was oxidase negative and did not reduce nitrate to nitrite. This strain was identified as V. metschnikovii with a Vitek 2 GN card (bioMérieux) and ID 32 E gallery (bioMérieux), and was confirmed by sequencing the 16S rRNA gene at the National Center for Microbiology.

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Vibrio metschnikoviiPneumonia

Cited by 22 publications

References 9 publications

Purification and characterization of a novel alkaline serine protease secreted by Vibrio metschnikovii

Purification and characterization of a novel alkaline serine protease secreted by Vibrio metschnikovii

Optimization of Multilocus Sequence Analysis for Identification of Species in the Genus Vibrio

Vibrio metschnikovii from a human infected leg ulcer

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