Purification and characterization of a novel alkaline serine protease secreted by Vibrio metschnikovii

Abstract

Abstract.A novel extracellular alkaline serine protease secreted by Vibrio metschnikovii (V. metschnikovii) ATCC700040 cells was purified by three chromatographic steps and characterized in terms of enzymatic kinetics and substrate specificity. The purified enzyme (named AKP-Vm) was composed of a single polypeptide with an apparent molecular weight of 50 kDa on 12% SDS-polyacrylamide gel in the presence of CuCl 2 . The optimal temperature and the pH for the enzyme were found to be 37˚C and 9.5, respectively. However, the enzyme activity was inhibited by inhibitors such as PMSF and aprotinin. AKP-Vm could hydrolyze a peptide bond at the carboxyl side of the arginine residue, as revealed by its amidolytic activity toward a chromogenic substrate, Boc-Val-Pro-Arg-pNA. . AKP-Vm protease could cleave various blood coagulation-associated proteins, including fibrinogen, prothrombin and thrombin. In particular, the enzyme showed powerful fibrinogenolytic and fibrinolytic activities, as it could cleave all major chains of fibrinogen and also digest cross-linked fibrin. The results obtained suggest that AKP-Vm is a novel alkaline serine protease that can actively cleave fibrinogen and cross-linked fibrin.

Detergent-, solvent- and salt-compatible thermoactive alkaline serine protease from halotolerant alkaliphilic Bacillus sp. NPST-AK15: purification and characterization

“…Generally, the molecular masses of alkaline proteases from microorganisms ranged from 15 to 30 kDa (Deng et al 2010;Joshi and Satyanarayana 2013;Raval et al 2014;Gohel and Singh 2015) with few exceptions. For example, alkaline protease reported from Vibrio metschnikovii ATCC700040 had a molecular weight of 50 kDa (Park et al 2012), and that from Stenotrophomonas maltophilia had a molecular weight of 98 kDa (Waghmare et al 2015).…”