Vibrio species involved in seafood-borne outbreaks (Vibrio cholerae, V. parahaemolyticus and V. vulnificus): Review of microbiological versus recent molecular detection methods in seafood products

Bonnin-Jusserand, Maryse; Copin, S.; Bris, Cédric Le; Brauge, Thomas; Gay, Mélanie; Brisabois, Anne; Grard, Thierry; Midelet, Graziella

doi:10.1080/10408398.2017.1384715

Cited by 134 publications

(109 citation statements)

References 113 publications

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“…Bacteria belonging to the Vibrio genus, are highly abundant in aquatic environments ( Thompson et al, 2004 ). Some of them are well-known enteric human pathogens such as Vibrio parahaemolyticus , V. vulnificus , or even V. cholerae , known as the causative agent of pandemic cholera ( Bonnin-Jusserand et al, 2017 ). Other Vibrio species are pathogenic to aquatic animals such as several species belonging to the Harveyi clade.…”

Section: Introductionmentioning

confidence: 99%

Rapid Identification of Vibrio Species of the Harveyi Clade Using MALDI-TOF MS Profiling With Main Spectral Profile Database Implemented With an In-House Database: Luvibase

et al. 2020

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Vibrio bacteria, and particularly members of the Harveyi clade, are the causative agents of vibriosis. This disease is responsible for mass mortality events and important economic losses on aquaculture farms. Improvements in surveillance and diagnosis are needed to successfully manage vibriosis outbreaks. 16S rRNA gene sequencing is generally considered to be the gold standard for bacterial identification but the cost and long processing time make it difficult to apply for routine identification. In contrast, MALDI-TOF MS offers rapid diagnosis and is commonly used in veterinary laboratories today. The major limiting factor for using this technique is the low environmental bacterial diversity in the commonly available databases. Here, we demonstrate that the sole use of the commercially available Bruker BioTyper database is not fully adequate for identifying Vibrio bacteria isolated from aquaculture farms. We therefore developed a new in-house database named Luvibase, composed of 23 reference MALDI-TOF mass spectra profiles obtained from Vibrio collection strains, mostly belonging to the Harveyi clade. The comparison of the accuracy of MALDI-TOF MS profiling and 16S rRNA gene sequencing revealed a lack of resolution for 16S rRNA gene sequencing. In contrast, MALDI-TOF MS profiling proved to be a more reliable tool for resolving species-level variations within the Harveyi clade. Finally, combining the Luvibase with the Bruker ver.9.0.0.0 database, led to successful identification of 47 Vibrio isolates obtained from moribund abalone, seabass and oysters. Thus, the use of Luvibase allow for increased confidence in identifying Vibrio species belonging to the Harveyi clade.

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Section: Introductionmentioning

confidence: 99%

Rapid Identification of Vibrio Species of the Harveyi Clade Using MALDI-TOF MS Profiling With Main Spectral Profile Database Implemented With an In-House Database: Luvibase

et al. 2020

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“…is a Gram-negative halophilic bacteria that belongs to the Vibrionaceae family [3][4][5][6][7][8] . They naturally inhabit the aquatic surroundings and associated with aquatic animals for example crustaceans, molluscs and fish [9][10][11][12][13] .…”

Section: Introductionmentioning

confidence: 99%

Genome sequence of Vibrio sp. OULL4 isolated from shellfish

Letchumanan¹,

Tan²,

Yin³

et al. 2020

Prog Micobes Mol Biol

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The members of Vibrionaceae family are Gram-negative bacterium are ubiquitous in marine and estuarine environments. This diverse group of bacteria include many pathogenic strains that potentially cause infection to humanand aquaculture animals. Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus are among the few recognized as a major, worldwide cause gastroenteritis, particularly in countries where seafood consumption is high. The control of these vibrios has been a hurdle due to the rising numbers of antibiotic resistant strains in the environments. We report the genome sequence of Vibrio sp. OULL4 isolated from shellfish. The availability of this genome sequence will facilitate the study of its antimicrobial traits, as well as add our knowledge of Vibrio sp. diversity and evolution.

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“…Migratory birds carry and transmit a wide range of in uenza A viruses in nature, causing international attention to the migratory birds health problems [3]. Aquatic products are mostly carriers of Vibrio, and migratory birds eat aquatic products such as shell sh and sh [4]. It discoveried, migratory birds were new carriers of V. cholerae and V. parahaemolyticus [5,6].…”

Section: Introductionmentioning

confidence: 99%

Bacterial Community Research of Migratory Bird in Chifeng, Neimeng via High-Throughput Sequencing

Zheng

Zhu

Guan

et al. 2020

Preprint

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Background: In August 2018, a large number of migratory birds died in Chifeng, Neimeng. We were entrusted by local animal disease prevention and control center to collect the migratory bird epidemic materials and their living environmental water, and in 2019, we collect the local migratory bird stool and their living environmental water again. The bacterial communities in migratory bird epidemic materials, water and aquatic plants are profifiled by high-throughput sequencing of the V3–V4 hypervariable region of 16S rDNA gene.Results: We found that the dominant phylum between migratory bird epidemic materials, water and aquatic plant were Proteobacteia, Bacteroidetes, Firmicutes, Fusobacteria and Verrucomicrobia in 2018. One year later, we found that the dominant phylum between migratory bird stool, water and aquatic plant were Proteobacteia, Firmicutes, Cyanobacteria, Bacteroidetes. The relative abundance among bacterial phylum notably differed between two years. The relative abundance of Fusobacteria and Verrucomicrobia were higher in samples in 2018, while that of Cyanobacteria was higher in water, 2019. The relative abundance of Fusobacteria in migratory bird epidemic materials began to decline in the later period over time. At genus level, the relative abundance of Vibrio, Clostridium and other patnogenic bacteria decreased markedly and disappeared in 2019. The salt content and pH show a downward trend.Conclusions: Differences in diet and geographical location can lead to diversification in migratory bird intestinal flora. It is necessary to pay attention to diversification in intestinal flora of migratory birds, especially the abundance of vibrio in intestine. The overall structure of intestinal flora and relative abundance changes of various species are showed intuitively by 16S rDNA amplicon. But comparing to PCR which using specific primers, the accuracy and sensitivity are relatively poor. In the case of purposeful detection, it can be used in combination with both 16S rDNA amplicon and PCR which using specific primers. They are more accurately, because of supplement each other.

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Vibrio species involved in seafood-borne outbreaks (Vibrio cholerae, V. parahaemolyticus and V. vulnificus): Review of microbiological versus recent molecular detection methods in seafood products

Cited by 134 publications

References 113 publications

Rapid Identification of Vibrio Species of the Harveyi Clade Using MALDI-TOF MS Profiling With Main Spectral Profile Database Implemented With an In-House Database: Luvibase

Rapid Identification of Vibrio Species of the Harveyi Clade Using MALDI-TOF MS Profiling With Main Spectral Profile Database Implemented With an In-House Database: Luvibase

Genome sequence of Vibrio sp. OULL4 isolated from shellfish

Bacterial Community Research of Migratory Bird in Chifeng, Neimeng via High-Throughput Sequencing

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