<i>Vibrio harveyi</i>
 isolated from cage-cultured seabass <i>Lates calcarifer</i>
 Bloch in the Philippines

Tendencia, Eleonor A.

doi:10.1046/j.1365-2109.2002.00688.x

Cited by 57 publications

(36 citation statements)

References 7 publications

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“…Vibrio harveyi, a Gram-negative bioluminescent marine bacterium of the family Vibrionaceae, causes Vibriosis, a highly virulent disease that has devastating effects on fish and prawnfarming industries worldwide (1)(2)(3)(4)(5). Through its ability to grow rapidly under both aerobic and anaerobic conditions, V. harveyi has a crucial role in the rapid turnover of chitin biomaterials in marine ecosystems.…”

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“…We recently identified chitoporin from V. harveyi (known as VhChiP) as a pore-forming channel that performs highly specific translocation of chitooligosaccharides (9,10). Single channel recordings using a black lipid membrane (BLM) 4 reconstitution technique showed that VhChiP inserted into the artificial bilayer membranes and formed a trimeric channel that remained steadily open under applied potentials of up to Ϯ150 mV. The fully open channel exhibited an average conductance of 1.8 Ϯ 0.13 nS in 1 M KCl, which is much larger than the average conductance of maltoporin (also called LamB) (0.15 nS in 1 M KCl) from Escherichia coli (11).…”

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Chitoporin from the Marine Bacterium Vibrio harveyi

Chumjan¹,

Winterhalter

Schulte

et al. 2015

Journal of Biological Chemistry

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Background: VhChiP is a sugar uptake channel specific for chitohexaose. Results: Mutations of Trp 136 , located at the entrance of the transmembrane pore, affect ion and sugar transport through VhChiP. Conclusion: Trp 136 regulates chitooligosaccharide uptake through VhChiP. Significance: Chitin uptake by the highly virulent bacterium V. harveyi through VhChiP is dependent on hydrophobic interactions between the sugar molecule and the channel surface.

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Chitoporin from the Marine Bacterium Vibrio harveyi

Chumjan¹,

Winterhalter

Schulte

et al. 2015

Journal of Biological Chemistry

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“…Bacteria from the genus Vibrio are frequently isolated from outbreaks in marine fish such as V. alginolyticus in the gilthead seabream Sparus aurata (Akaayli et al, 2008), V. harveyi in seabass Lates calcarifer (Tendencia, 2002) and in the summer flounder Paralichthys dentatus (Gauger et al, 2006), V. pelagius in turbot Scophthalmus maximus (Villamil et al, 2003), V. splendidus and V. scophthalmi in common dentex Dentex dentex (Sitjà-Bobadilla et al, 2007). In sea horses, Alcaide et al (2001) reported V. harveyi from an outbreak in Hippocampus kuda.…”

Section: Resultsmentioning

confidence: 99%

Isolation and experimental infection with Vibrio alginolyticus in the sea horse, Hippocampus reidi Ginsburg, 1933 (Osteichthyes: Syngnathidae) in Brazil

et al. 2010

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The aim of this study was to evaluate the pathogenicity of Vibrio alginolyticus isolated from an outbreak of sea horse Hippocampus reidi reared in the State of Santa Catarina, Brazil, by experimental infection. Sea horses with necrosis on the mouth epithelium were collected from aquaria at the Aquaculture Department, UFSC and the bacterium isolated from the mouth, liver, heart and blood in tiosulphate citrate bilesalt sucrose agar broth. The strains were identified by API 20E kit with 99.1% probability as Vibrio alginolyticus. Twelve adult sea horses (9.63 ± 2.42 g and 15.12 ± 0.87 cm) were distributed in six aquaria of 10 L capacity with aerated sea water. Fish from three aquaria were submitted to an immersion bath in a solution containing 1.0 × 10 7 CFU of V. alginolyticus/mL for 15 minutes. Fish from the other three aquaria received the same procedure without bacteria. Twenty four hours after this challenge, 100% mortality was observed in the animals infected with V. alginolyticus. No mortality was observed in non-infected fish. Hyperplasia, displacement and fusion of secondary lamellae of the gills; leukocyte infiltration and necrotic foci in the kidney; hyperplasia, sinusoidal deformation and necrotic foci in the liver were observed in histopathological analysis. The V. alginolyticus isolated in this study was pathogenic to H. reidi and constitutes an important sanitary problem to its culture.Keywords: experimental infection, vibriosis, histopathology. Isolamento e infecção experimental de Vibrio alginolyticus em cavalo-marinho,Hippocampus reidi Ginsburg, 1933 (Osteichthyes: Syngnathidae) no Brasil ResumoFoi avaliado por meio de infecção experimental a patogenicidade de Vibrio alginolyticus isolado de um surto de enfermidade em cavalo-marinho Hippocampus reidi cultivado no Estado de Santa Catarina, Brasil. Os peixes com necroses no epitélio bucal foram coletados em aquários do Departamento de Aquicultura, UFSC e as bactérias isoladas da boca, fígado, coração e sangue em meio Agar tiossulfato citrato bile sacarose. Os isolados foram identificados pelo kit API 20E como Vibrio alginolyticus com 99,1% de probabilidade. Doze peixes adultos (9,63 ± 2,42 g e 15,12 ± 0,87 cm) foram distribuídos em seis aquários de 10 L com água marinha e aeração. Peixes de três aquários foram submetidos a um banho de imersão por 15 minutos em uma solução contendo 1,0 × 10 7 UFC de V. alginolyticus/ mL. Nos outros três aquários realizou-se o mesmo procedimento sem a bactéria. Vinte e quatro horas após o desafio, 100% de mortalidade foi observada nos animais infectados com V. alginolyticus. Não houve mortalidade nos peixes não infectados. Nas análises histopatológicas, foi observado hiperplasia, deslocamento do epitélio e fusão das lamelas secundárias das brânquias; infiltração de leucócitos e necrose no rim; hiperplasia, deformação sinusoidal e necrose no fígado nos animais desafiados com V. alginolyticus. O V. alginolyticus isolado neste estudo foi patogênico para H. reidi, constituindo-se de um importante problema para seu...

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“…Penaeid shrimp is particularly susceptible to V. harveyi, and, upon V. harveyi infection, develops a disease called luminous vibriosis that can result in heavy economic losses [3][4][5][6][7][8]. In addition to shrimp, fish, lobster, and abalone are also known to be affected by V. harveyi-related vibriosis [9][10][11][12].…”

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confidence: 99%