<i>Vibrio damsela</i>
            , a Marine Bacterium, Causes Skin Ulcers on the Damselfish
            <i>Chromis punctipinnis</i>

Love, Milton S.; Teebken-Fisher, Dixie; Hose, Jo Ellen; Farmer, J. J.; Hickman, F W; Fanning, G R

doi:10.1126/science.214.4525.1139

Cited by 185 publications

(111 citation statements)

References 5 publications

Supporting

Mentioning

101

Contrasting

Unclassified

Order By: Relevance

“…This renewed effort has led to the recent reorganization of the genus Vibrio (2) and to descriptions of a number of new species (3-5, 7,8,10).…”

mentioning

confidence: 99%

Vibrio aestuarianus: a New Species from Estuarine Waters and Shellfish

Tison¹,

Seidler

1983

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

A group of phenotypically similar bacteria were isolated from estuarine waters and shellfish from the Oregon coast by procedures designed for the isolation of potentially pathogenic Vibrio species. Although these strains were typical of bacteria of the genus Vibrio, they could be distinguished from previously described species by their arginine di hydrolase activities, negative Voges-Proskauer reactions, fermentation of sucrose and lactose, and inability to grow in 0 or >5% added NaCl. The deoxyribonucleic acid base compositions of these strains ranged from 43 to 44 mol% guanine plus cytosine. There was >85% deoxyribonucleic acid relatedness among these strains (melting temperature -15°C; membrane filter competition method), whereas there was <25% relatedness with other Vibrio species, including Vibria anguillarum, Vibrio nereis, and Vibrio splendidus, which are also arginine dihydrolase positive and have similar guanine-pluscytosine contents. Our results indicate that the strains which we studied are distinct from previously described species of the genus Vibrio, and we propose the name Vibrio aestuarianus for these strains, indicating their estuarine origin. The type strain is strain OY-0-002 (= ATCC 35048).The recent realization that Vibrio species pathogenic to humans were endemic to United States coastal waters has led to a renaissance in the study of the ecology, pathogenicity, and taxonomy of these bacteria. This renewed effort has led to the recent reorganization of the genus Vibrio (2) and to descriptions of a number of new species (3-5, 7, 8, 10).During the course of a survey to determine the distribution of pathogenic Vibrio spp. in estuarine waters and shellfish from the coast of Oregon (D. L. Tison, M. Nishibuchi, and R. J. Seidler, Abstr. Annu. Meet. Am. SOC. Microbi-01. 1981, Q97, p. 216), phenotypically similar bacteria which were distinct from previously described Vibrio species were isolated. Comparisons with other lactose-fermenting Vibrio species indicated that the arginine dihydrolase-positive, lactose-positive strains isolated from Oregon estuaries were probably a new Vibrio species (11).In this paper we describe the characteristics of these strains and show that these bacteria were genetically distinct from other phenotypically similar Vibrio species, thereby warranting their designation as a new species, Vibrio aestuarianus MATERIALS AND METHODSBacterial strains. Strains from water and shellfish from the Oregon coast were isolated by inoculating alkaline peptone broth and subsequently plating and isolating the organisms on thiosulfate-citrate-bile saltssucrose agar (Oxoid) (6). The sources of V . aestuarianus and the reference strains used in this study are listed in Table 1.Phenotypic and genetic characterization. The media and methods used in phenotypic characterizations, deoxyribonucleic acid (DNA) base composition determinations, and DNA-DNA hybridization studies were essentially as described previously (12, 13). RESULTS AND DISCUSSIONVibrio aestuarianus sp. nov. The type strain of V...

show abstract

“…This renewed effort has led to the recent reorganization of the genus Vibrio (2) and to descriptions of a number of new species (3-5, 7,8,10).…”

mentioning

confidence: 99%

Vibrio aestuarianus: a New Species from Estuarine Waters and Shellfish

Tison¹,

Seidler

1983

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

show abstract

“…In addition, we have recently demonstrated (Fouz and Toranzo, unpublished data) that some of these vibrios are V. damsela which was described as a common pathogen from fish, reptiles and mammals (Blake et al, 1980;Love et al, 1981). Occasionally, the external signs of these atypical vibrioses in turbot are lesions centered on the mouth and head which create clinical manifestations similar to the enteric redmouth syndrome of salmonids infected by Yersinia ruckeri.…”

Section: Resultsmentioning

confidence: 99%

Epizootic study in a turbot farm: bacteriology, virology, parasitology and histology

Novoa¹,

Nún˜ez²,

Fernández-Puentes³

et al. 1992

Aquaculture

View full text Add to dashboard Cite

show abstract

“…V. carchariaedid not contain detectable plasmids. Interestingly, V. damsela did contain one plasmid band, the 30 Mdal band, that was identical to that detected in the type strain of V. damsela (strain CDC-2588-80, Love et al, 1981). We are currently examining other V. damsela strains for plasmid content, using both whole plasmid analysis and restriction endonuclease analysis.…”

Section: Description Of Isolatesmentioning

confidence: 99%

“…Previously, V. damsela was reported by Love et al (1981) to have a narrow host range, infecting only the blacksmith (Chromis punctipinnis) and the garibaldi (Hypsypops rubicunda). Thus, this report extends the host range for V. damsela to include the spiny dogfish and brown shark, and lemon shark.…”

Section: Virulence Studiesmentioning

confidence: 99%

Vibrio species as agents of elasmobranch disease

Grimes

Colwell

Stemmler

et al. 1984

Helgolander Meeresunters

View full text Add to dashboard Cite

Two Vibrio species identified as V. damsela and a new sucrose-positive Vibrio sp., V. carchariae sp. nov., were simultaneously isolated from a brown shark which died while being held in captivity at a large aquarium. Pathogenicity studies were subsequently conducted using a variety of elasmobranchs, including smooth dogfish and lemon sharks. Both bacterial strains proved pathogenic, causing death in nearly all of the elasmobranch hosts challenged. Virulence studies revealed that both bacterial strains were cytotoxic for Y-I mouse adrenal cells. The V. damsela strain was highly cytotoxic, causing Y-1 cellular damage at culture supernatant dilutions up to 1 : 128. Both strains were hemolytic, but neither exhibited the Kanagawa phenomenon. They were both capable of urea hydrolysis, an interesting trait, considering that elasmobranchs retain large (ca 300 milliosmolal) urea concentration in their tissue.

show abstract

Vibrio damsela , a Marine Bacterium, Causes Skin Ulcers on the Damselfish Chromis punctipinnis

Cited by 185 publications

References 5 publications

Vibrio aestuarianus: a New Species from Estuarine Waters and Shellfish

Vibrio aestuarianus: a New Species from Estuarine Waters and Shellfish

Epizootic study in a turbot farm: bacteriology, virology, parasitology and histology

Vibrio species as agents of elasmobranch disease

Contact Info

Product

Resources

About