Vibrio alginolyticus influences quorum sensing-controlled phenotypes of acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus

Abstract

Background Acute hepatopancreatic necrosis syndrome (AHPND) caused by Vibrio parahaemolyticus strain (VPAHPND) impacts the shrimp industry worldwide. With the increasing problem of antibiotic abuse, studies on quorum sensing (QS) system and anti-QS compounds bring potential breakthroughs for disease prevention and treatment. Methods In this study, the cell-free culture supernatant (CFCS) and its extract of V. alginolyticus BC25 were investigated for anti-QS activity against a reporter bacteria, Chromobacterium violaceum DMST46846. The effects of CFCS and/ or extract on motility, biofilm formation and extracellular polymeric substances (EPSs) of VPAHPND PSU5591 were evaluated. Moreover, the effects of V. alginolyticus BC25 on virulence of VPAHPND PSU5591 were investigated by shrimp challenge test. The potentially active anti-QS compounds presented in the extract and effect on gene expression of VPAHPND PSU5591 were identified. Results The CFCS of V. alginolyticus BC25 and its extract showed a significant anti-QS activity against the reporter bacteria as well as swimming and swarming motilities, biofilms, and EPSs production by VPAHPND PSU5591. Transcriptome analysis revealed that V. alginolyticus BC25 extract significantly reduced the flagella genes involved in biofilm formation and iron-controlled virulence regulatory gene of VPAHPND PSU5591. Whereas, the LuxR family transcriptional regulator gene, c-factor, a cell-cell signaling gene, and capsular polysaccharide were up-regulated. The potentially active anti-QS compounds identified in extract were Cyclo-(L-Leu-L-Pro), and Cyclo-(L-Phe-L-Pro). Furthermore, V. alginolyticus BC25 enhanced disease resistance against VPAHPND PSU5591 in tested shrimp larvae. Conclusion These findings suggest that V. alginolyticus BC25 could provide natural anti-QS and anti-biofilms compounds and has great ability to be used as biocontrol agent against VPAHPND infection in shrimp aquaculture.

aTAP: automated transcriptome analysis platform for processing RNA-seq data by de novo assembly

“…To test the analysis of a dataset without replicates, we used the study of Paopradit et al. [ 41 ]. They examined the effect of Vibrio alginolyticus in quorum sensing-controlled phenotypes of acute hepatopancreatic necrosis disease using microbiological approaches and RNA-seq.…”

Expression of the AHPND Toxins PirAvp and PirBvp Is Regulated by Components of the Vibrio parahaemolyticus Quorum Sensing (QS) System

“…AphB is also a positive regulator of LuxR/OpaR activity, and it activates the expression of the exotoxin Asp [ 20 ]. These studies, together with the recent finding that anti-QS compounds may reduce AHPND pathogenicity [ 18 ], all suggest that the QS system might be critically important for regulating the virulence of AHPND-causing bacteria.…”

“…QS-regulated genes are involved in many important physiological activities, such as biofilm formation, bioluminescence, virulence factor production, conjugation, plasmid transfer, antibiotic production, cell mobility, and sporulation [ 15 ]. The importance of the QS system in AHPND pathogenicity was also recently demonstrated: extract from V. alginolyticus BC25 contained the anti-QS compounds Cyclo-(L-Leu-L-Pro) and Cyclo-(L-Phe-L-Pro), both of which had anti-QS activity, and pre-treatment with V. alginolyticus BC25 reduced mortality after challenge with the AHPND-causing strain V. parahaemolyticus PSU5591 [ 18 ]. However, this study did not investigate the mechanism by which the QS system regulates the virulence of AHPND-causing V. parahaemolyticus .…”

What Happened Over the Last 10 Years (2012 to 2021): A Bibliometric Analysis of Acute Hepatopancreatic Necrosis Disease (AHPND) Research in Southeast Asia

“…As shown in Figure 9, recent advances in AHPND research in Southeast Asia include the characterization of non-Vibrio parahaemolyticus species as causative agents of AHPND (De Guia et al, 2020;Dong et al, 2017;Muthukrishnan et al, 2019). As time progressed, research on AHPND focused on combating the disease (Santos et al, 2020), immune response of shrimp against AHPND upon supplementation with immunostimulants (Alonzo et al, 2017;Arabit et al, 2017), generation of polyclonal antibodies (Nguyen-Phuoc et al, 2021), the role of quorum sensing (Paopradit et al, 2021), and the use of bioactive compounds to target the mechanisms involved in the pathogenesis of AHPND (Dadi et al, 2021;Guzman et al, 2021;Karnjana et al, 2019Karnjana et al, , 2020Nguyen Thi Truc et al, 2019;Soowannayan, Boonmee, et al, 2019;Soowannayan, Chandra Teja, et al, 2019;Yatip et al, 2018).…”