<i>Trichophyton</i>species susceptibility to green and red propolis from Brazil

Siqueira, Ana Beatriz Sotero; Gomes, Bruno; Cambuim, Idalina Inês Fonsêca Nogueira; Maia, Raquel Cristina; Abreu, Sheila Lemos; Souza-Motta, Cristina Maria de; Queiroz, L. A.; Porto, Ana Lúcia Figueiredo

doi:10.1111/j.1472-765x.2008.02494.x

Cited by 53 publications

(35 citation statements)

References 33 publications

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“…2, 3 and 4) treatments with propolis allowed a lower contamination of eggs than the control treatment, and propolis concentrations of 2,400 µg and 240 µg were more efficient than formaldehyde. The propolis antifungal (fungistatic and fungicide) activity is attributed to the phenolic acids (cinnamic, feluric and caffeic acids), terpenes and flavonoids like chrysin, ermanina, galangin, kaempferol, pinobanskina and mainly pinocembrine (SiQueira et al, 2009). These substances are found in green propolis (marcucci et al, 2001;cuSHnie;lamB, 2005).…”

Section: Discussionmentioning

confidence: 99%

Propolis: a natural product as an alternative for disinfection of embryonated eggs for incubation

et al. 2012

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During the cooling process of embryonated eggs, there is a natural air flux from the surface to the inner part of the eggs, carrying contaminants such as bacteria and fungi through the shell's pores, infecting embryos and resulting in the inability to hatch or poor chick quality. Formaldehyde, a toxic product, is still the most used disinfectant for embryonated eggs in the aviculture industry. In order to evaluate the antimicrobial activity of the green propolis ethanolic extract as an alternative to formaldehyde, 140 hatching eggs from laying hens were collected and submitted to disinfection with five different treatments: T1 -without disinfection; T2 -formaldehyde fumigated eggs; T3, T4 and T5 disinfection by immersion in propolis solution in the concentrations of 2,400 µg, 240 µg and 24 µg, respectively. The contamination levels by total mesophiles and fungi of the egg shells (Aspergillus sp. and other moulds) after disinfection with propolis were lower than when compared to the control without disinfection. In comparison with formaldehyde, the 240 µg and 24 µg propolis concentrations did not differ regarding antibacterial activity, but for antifungal activity the 2,400 µg and 240 µg concentrations were more efficient. The 2,400 µg and 240 µg propolis treatments presented a hatching rate of 94.1%, compared to only 84.6% for the formaldehyde treatment. The green propolis ethanolic extract presented antibacterial and antifungal activities in embryonated eggs showing that it can be a new natural disinfectant product substituting formaldehyde.KEY WORDS: Propolis, embryonated eggs, formaldehyde, disinfectants. RESUMO PRÓPOLIS: UM PRODUTO NATURAL COMO ALTERNATIVA PARA DESINFECÇÃO DE OVOS EMBRIONADOS PARA INCUBAÇÃO. Durante o processo de resfriamento dos ovos embrionados, há um fluxo natural de ar da superfície para o interior dos ovos carreando contaminantes como bactérias e fungos, por meio dos poros da casca, infectando o embrião e resultando na inabilidade para eclodir e pintinhos de má qualidade. O formaldeído que é um produto tóxico ainda é o desinfetante mais utilizado para a desinfecção de ovos embrionados pela indústria avícola. Para avaliar a atividade antimicrobiana do extrato etanólico da própolis verde, como alternativa ao formaldeído, foram coletados 140 ovos de ninhos de matrizes de frango de corte submetidos à desinfecção com cinco tratamentos: T1 -sem desinfecção; T2 -ovos fumigados com formaldeído; T3, T4 e T5 desinfetados por imersão com solução de própolis nas concentrações de 2.400 µg, 240 µg e 24 µg, respectivamente. Os níveis de contaminação da casca dos ovos por mesófilos totais e fungos (Aspergillus e outros bolores), após a desinfecção com própolis, foram menores quando comparados ao controle. Na comparação ao tratamento com formaldeído as concentrações de própolis com 240 µg e 24 µg não diferiram para atividade antibacteriana, já para atividade antifúngica, 2,4 mg e 240 µg foram superiores. Com relação à eclodibilidade dos ovos, após 21 dias de incubação, os tratamentos de própolis...

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Section: Discussionmentioning

confidence: 99%

Propolis: a natural product as an alternative for disinfection of embryonated eggs for incubation

et al. 2012

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“…Propolis is a useful substance in medicine. In the literature, propolis has various pharmacological activities such as antiparasitic (Duran et al 2008), antibacterial (Onlen et al 2007a, b;Duran et al 2006;Oksuz et al 2005;Onlen et al 2007a, b), antifungal (Siqueira et al 2009), antiviral (Shimizu et al 2008), antiinflammatory (McLennan et al 2008, immunostimulatory (Cuesta et al 2005), and anti-carcinogenetic activities (Diaz-Carballo et al 2008).…”

Section: Introductionmentioning

confidence: 97%

GC-MS analysis and antileishmanial activities of two Turkish propolis types

et al. 2010

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Propolis is a honeybee product with a very complex chemical composition and various pharmacological properties. This study was aimed to investigate antileishmanial activities of "Bursa" and "Hatay" propolis samples against Leishmania infantum and Leishmania tropica strains. Propolis samples were analysed with the gas chromatography-mass spectrometry technique. Promastigotes were incubated in Roswell Park Memorial Institute culture medium in the absence and presence of several concentrations (50, 100, 250, 500, 750, and 1,000 μg/mL) of each propolis sample. The viability and cell morphology of promastigotes in each concentration were examined after 24, 48, 72, and 96 h of incubation. The growth of leishmania parasites was significantly suppressed in the presence of 500, 750, and 1,000 μg/mL of Hatay propolis. Bursa propolis was found to be efficient in inhibiting the growth of leishmania promastigotes in culture media at these concentrations, 250, 500, 750, and 1,000 μg/mL. Thus, the in vitro results showed that the Hatay and Bursa propolis samples decreased significantly the proliferation of L. infantum and L. tropica parasites (p < 0.001); however, Bursa propolis was found to be more effective than Hatay propolis against leishmania promastigotes. These two natural products may be useful agents in the prevention of leishmanial infections.

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“…Propolis has cytotoxic (53), anti-herpes virus (83), antitumor (34), radical scavenging (4), antimicrobial (38,60), antiprotozoan (55), and anti-HIV (30) activity and suppressive effects of dioxin toxicity (60). More recently, evidence has shown that propolis can be used to treat Candida fungal infections (1,17,59,68,76).…”

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confidence: 99%

Molecular Characterization of Propolis-Induced Cell Death in Saccharomyces cerevisiae

et al. 2011

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Propolis, a natural product of plant resins, is used by the bees to seal holes in their honeycombs and protect the hive entrance. However, propolis has also been used in folk medicine for centuries. Here, we apply the power of Saccharomyces cerevisiae as a model organism for studies of genetics, cell biology, and genomics to determine how propolis affects fungi at the cellular level. Propolis is able to induce an apoptosis cell death response. However, increased exposure to propolis provides a corresponding increase in the necrosis response. We showed that cytochrome c but not endonuclease G (Nuc1p) is involved in propolis-mediated cell death in S. cerevisiae. We also observed that the metacaspase YCA1 gene is important for propolis-mediated cell death. To elucidate the gene functions that may be required for propolis sensitivity in eukaryotes, the full collection of about 4,800 haploid S. cerevisiae deletion strains was screened for propolis sensitivity. We were able to identify 138 deletion strains that have different degrees of propolis sensitivity compared to the corresponding wild-type strains. Systems biology revealed enrichment for genes involved in the mitochondrial electron transport chain, vacuolar acidification, negative regulation of transcription from RNA polymerase II promoter, regulation of macroautophagy associated with protein targeting to vacuoles, and cellular response to starvation. Validation studies indicated that propolis sensitivity is dependent on the mitochondrial function and that vacuolar acidification and autophagy are important for yeast cell death caused by propolis.

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Trichophytonspecies susceptibility to green and red propolis from Brazil

Cited by 53 publications

References 33 publications

Propolis: a natural product as an alternative for disinfection of embryonated eggs for incubation

Propolis: a natural product as an alternative for disinfection of embryonated eggs for incubation

GC-MS analysis and antileishmanial activities of two Turkish propolis types

Molecular Characterization of Propolis-Induced Cell Death in Saccharomyces cerevisiae

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