Trans‐18:1 and CLA Isomers in Rumen and Duodenal Digesta of Bulls Fed n‐3 and n‐6 PUFA‐Based Diets

Abstract

The aim of the study was to investigate the effect of n-6 PUFA (maize silage/grass silage, soybean meal and soybean oil, control) and n-3 PUFA (grass silage, rapeseed cake and rapeseed oil, experiment) based diets on the occurrence of rumen- and duodenal digesta trans-C18:1 and CLA isomers of German Simmental bulls. The results based on rumen and duodenal digesta samples immediately taken from the bulls just after slaughter. The diet affected the occurrence of individual trans-C18:1 and CLA isomers in the rumen and duodenal digesta in different ways. The isomer trans-11,cis-13 CLA was detected as the most abundant isomer in the rumen of n-3 PUFA based diet fed bulls compared to n-6 PUFA based diet fed bulls. The trans-7,cis-9 CLA isomer was not detected in the rumen samples of bulls fed both diets, however abundant trans-7,cis-9 CLA was identified and quantified in the duodenum digesta. Both the concentration of the sum of trans-18:1 fatty acids and individual isomers in the rumen were not affected by the diet, except trans-16-18:1. The concentration of trans-16-18:1 was significantly decreased in the rumen of n-3 PUFA supplemented-fed bulls compared to n-6 PUFA supplemented-fed bulls.

Lipid metabolism in the rumen: New insights on lipolysis and biohydrogenation with an emphasis on the role of endogenous plant factors

“…Bacteria usually has the highest genetic diversity (Nam and Garnsworthy, 2007;Jenkins et al, 2008), and it is recognized that ruminal bacteria are mainly responsible for LP (Dawson et al, 1977;Jenkins et al, 2008;Lourenç o et al, 2010) and BH (Nam and Garnsworthy, 2007). However, contrary to expectations, protozoa also influence the content of BH intermediate by isomerase Or-Rashid et al, 2011;Shen et al, 2011) as do fungi (Nam and Garnsworthy, 2007). Results of Or-Rashid et al (2011) appear interesting in this context as their comparison of linoleic acid metabolism in bacteria, protozoa and their mixture showed that all metabolize linoleic acid to different CLA isomers and protozoa were incapable of biohydrogenating LA and, when tested in the bacterial/protozoal mixture, modulated BH of LA (Varadyova et al, 2008).…”

Addition of an extract of lucerne (Medicago sativa L.) to cattle diets – Effects on fatty acid profile, meat quality and eating quality of the M. longissimus muscle

“…The CLA isomers in the extracted total lipids of M. longissimus were analysed using silver ion high pressure liquid chromatography (HPLC) using an HPLC system (LC 10A, Shimadzu, Kyoto, Japan) equipped with a pump (LC-10AD VP), auto sampler (SIL-10AF), 50 µL injection loop, a photodiode array detector (SPD-M 10Avp, Shimadzu, Kyoto, Japan) operated at 233 nm, and a Shimadzu CLASS-VP software system (Version 6.12 SP4), as described in detail by Shen, Dannenberger, Nuernberg, Nuernberg, & Zhao (2011). Briefly, four ChromSpher 5 Lipids silver ion-impregnated columns (4.6 mm i.d.…”

Sea buckthorn pomace supplementation in the finishing diets of pigs – are there effects on meat quality and muscle fatty acids?

“…The fatty acid analysis of the muscle lipids was performed using capillary GC with a CP-Sil 88 CB column (100 m × 0.25 mm, Chrompack-Varian, Lake Forest, CA, USA) that was installed in a PerkinElmer gas chromatograph Clarus 680 with a flame ionisation detector (PerkinElmer Instruments, Shelton, CT, USA). Detailed GC conditions are described in Shen et al (2011). Hydrogen was used as the carrier gas at a flow rate of 1 mL min −1 .…”