<i>Terminalia tomentosa </i>Bark Ameliorates Inflammation and Arthritis in Carrageenan Induced Inflammatory Model and Freund’s Adjuvant-Induced Arthritis Model in Rats

Jitta, Srinivas Reddy; Daram, Prasanthi; Gourishetti, Karthik; Misra, Chandra Sekhar; Polu, Picheswara Rao; Shah, Abhishek; Shreedhara, CS; Nampoothiri, Madhavan; Lobo, Richard

doi:10.1155/2019/7898914

Cited by 23 publications

(18 citation statements)

References 14 publications

(20 reference statements)

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“…Arthritis condition generally results in accumulation of leukocytes and release of lysosomal enzymes, the main mediators in arthritis. In the present study, the migration of leukocytes in the inflamed area is significantly suppressed by the methanolic extract of Cissusquadrangularis as seen from the significant decrease in total WBC count 25,26 . The ESR level which drastically increased in the arthritic group has been remarkably counteracted by the drug, restoring it to near normal, to justifying its significant role in arthritic condition 27,28 .…”

Section: Discussionsupporting

confidence: 53%

Haematological Assessment of Methanolic Stem Extract of Cissus quadrangularisLinn. against Freund's Complete Adjuvant induced Female Albino Arthritic Rats

Shamina¹,

Anjana²,

Suja

2022

Int J Life Sci Pharm Res

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Rheumatoid arthritis is a chronic, inflammatory and autoimmune disorder. Rheumatoid arthritis is a joint destruction which occurs due to increased expression of cytokines and transcription factors. Cissus quadrangularis belongs to the Vitaceae family. The plant shows anti-osteoporotic, anti-inflammatory and anti-microbial properties. Soxhlet method was used for the extraction of the plant using methanol as a solvent. The anti-arthritic activity of methanolic extract of Cissus quadrangularis was evaluated by in vivo study. The present study assess the haematological parameters in female albino rats after the induction of Freund's Complete Adjuvant. Dexamethasone was used as the standard reference drug. The study showed varied changes in the haematology after the arthritic induction by 0.1 ml FCA which were analyzed before and afterthe treatment period. The inhibition of secondary inflammation in adjuvant arthritic rats by methanolic extract of Cissus quadrangularis was studied by haematological parameters. The total haemoglobin, total RBC, total WBC, ESR and RF values were assessed before initiation of the treatment and after for 28 days of the treatment. The results showed significant incline or decline in each parameter in the study period of 28 days. While their changes were rectified with the oral administration of methanolic extract of the plant at a dose of 250mg/kg. The methanolic extract boosted the levels of Hb, RBC and simultaneously reduced WBC, ESR levels along with the serological factor RF. The plant extract showed effective results in the haematological parameters, when compared to the standard reference drug Dexamethasone. The results from the study suggest that the plant has potential effect in curing arthritis.

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Section: Discussionsupporting

confidence: 53%

Haematological Assessment of Methanolic Stem Extract of Cissus quadrangularisLinn. against Freund's Complete Adjuvant induced Female Albino Arthritic Rats

Shamina¹,

Anjana²,

Suja

2022

Int J Life Sci Pharm Res

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“…This technique is carried out, as described in Chondrex [40]. In this model, BB rats aged 7-8 weeks, with a mature immune system are most appropriate.…”

Section: Collagen-induced Arthritismentioning

confidence: 99%

“…BB rats are the most suitable species because they show the quickest onset of arthritis, within 12-14 days. The extent of arthritis development can be determined using appropriate methods [40].…”

Section: Collagen-induced Arthritismentioning

confidence: 99%

In vitro and In vivo Models for Anti-inflammation: An Evaluative Review

Eze

Uzor

Peter

et al. 2019

INNOSC Theranostics and Pharmacological Sciences

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Anti-inflammatory activity study involves developing a model that mimics, or provokes the production or release of, the biochemical mediators of inflammation, and monitoring the response of these biochemicals to the test drugs. This report constitutes an updated review of the in vitro and in vivo study models for assessing anti-inflammatory activity in plant extracts and synthetic drugs. The materials, instrumentation, and methods involved, as well as the mechanism of anti-inflammatory activity tested in each model, are extensively described. The merits and limitations of each method have also been discussed. A comparative assessment of the in vivo animal models vis-à-vis, the in vitro enzyme models have been made to assist scientists and researchers in the choice of assay method in terms of sensitivity, reliability, duration of test, ethical, and cost considerations.

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“…Accumulating evidence indicates that κ-CGNs, especially their low-MW degradation products, induce inflammation in the colon, resulting in a range of diseases in cell and animal models, including thrombosis [28][29][30][31], peritonitis [26,[32][33][34], paw edema [35,36], pleurisy [37,38], gastroenterocolitis [39,40], arthritis [41,42], and inflammatory bowel disease. However, CGNs also show positive health effects, such as antitumor [43], antimicrobial [44], and antiviral [45,46].…”

Section: Discussionmentioning

confidence: 99%

Carrageenan oligosaccharides and their degrading bacteria induce intestinal inflammation in germ-free mouse

Yin

et al. 2020

Preprint

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Background: Carrageenans (CGNs) are widely used in food and pharmaceutical industries. However, the safety of CGNs is still under debate, because degraded CGNs have been reported to promote an intestinal inflammatory response in animal models. Here, we studied the relationship among CGNs, human gut microbiota, and the host inflammatory response.Methods: TLC was selected for detecting the degradation of KCPs by human gut microbiota in vitro batch fermentation system. PCR-DGGE and real time PCR were used for studying bacterial community. ESI-MS was used for KCPs structure analysis. Hematoxylin-eosin staining (HE), immunohistochemistry (IHC) and RNA-seq were used to evaluated the KCPs on host inflammation response in germ-free mice.Results: Thin-layer chromatography (TLC) data showed that CGNs with a molecular weight (Mw) higher than 100 kDa are not degraded by human fecal microbiota, but low Mw CGNs with an Mw around ~4.5 kDa (KCOs) could be degraded by seven of eight human fecal microbiota samples. KCO degrading B. xylanisolvens was isolated from fecal samples, and PCR-DGGE profiling with band sequencing suggested that B. xylanisolvens was the key KCO degrader in the human gut. Two putative κ-carrageenase genes were identified in the genome sequence of B. xylanisolvens. However, their function on KCO degrading was not verified in vitro. And the sulfate group from KCO is not removed after in vitro degradation by human fecal microbiota, as shown by ESI-MS analysis. The effects of KCO and KCO degrading bacteria on the inflammatory response were investigated in germ-free mice. Increased numbers of P-P38-, CD3a-, and CD79a-positive cells were found in the colon and rectum in mice fed with KCO plus KCO degrading bacteria than in mice fed with only KCO or only B. xylanisolvens and E. coli, as shown by RNA-Seq analysis, HE staining, and IHC. Conclusion: Our data suggested that the presence of KCO degrading bacteria promote the pro-inflammatory effects of CGNs.

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Terminalia tomentosa Bark Ameliorates Inflammation and Arthritis in Carrageenan Induced Inflammatory Model and Freund’s Adjuvant-Induced Arthritis Model in Rats

Cited by 23 publications

References 14 publications

Haematological Assessment of Methanolic Stem Extract of Cissus quadrangularisLinn. against Freund's Complete Adjuvant induced Female Albino Arthritic Rats

Haematological Assessment of Methanolic Stem Extract of Cissus quadrangularisLinn. against Freund's Complete Adjuvant induced Female Albino Arthritic Rats

In vitro and In vivo Models for Anti-inflammation: An Evaluative Review

Carrageenan oligosaccharides and their degrading bacteria induce intestinal inflammation in germ-free mouse

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