Terminalia belliricastimulates the secretion and action of insulin and inhibits starch digestion and protein glycationin vitro

Abstract

Traditional plant treatments have been used throughout the world for the therapy of diabetes mellitus. The aim of the present study was to investigate the efficacy and mode of action of Terminalia bellirica used traditionally for the treatment of diabetes in India. T. bellirica aqueous extract stimulated basal insulin output and potentiated glucose-stimulated insulin secretion concentration-dependently in the clonal pancreatic β-cell line, BRIN-BD11 (P < 0·001). The insulin-secretory activity of the plant extract was abolished in the absence of extracellular Ca2+ and by inhibitors of cellular Ca2+ uptake, diazoxide and verapamil (P < 0·001; n 8). Furthermore, the extract did not increase insulin secretion in depolarised cells and did not further augment insulin secretion triggered by tolbutamide or glibenclamide. T. bellirica extract also displayed insulin-mimetic activity and enhanced insulin-stimulated glucose uptake in 3T3-L1 adipocytes by 300 %. At higher concentrations, the extract also produced a 10–50 % (P < 0·001) decrease in starch digestion in vitro and inhibited protein glycation (P < 0·001). The present study has revealed that components in T. bellirica extract stimulate insulin secretion, enhance insulin action and inhibit both protein glycation and starch digestion. The former actions are dependent on the active principle(s) in the plant being absorbed intact. Future work assessing the use of T. bellirica as a dietary adjunct or as a source of active anti-diabetic agents may provide new opportunities for the treatment of diabetes.

Aqueous extract of some indigenous medicinal plants inhibits glycation at multiple stages and protects erythrocytes from oxidative damage–an in vitro study

“…The observed results for AGEs inhibition by T. bellirica , E. officinalis and A. indica extracts are supported by other recent studies (Gutierrez et al 2011;Nampoothiri et al 2011;Kasabri et al 2010). However inhibitory effects of the analyzed extracts were not limited to AGEs formation (which account for only a Glycation also induces the β aggregation of proteins and collectively leads to OS.…”

“…Inhibited albumin (Nampoothiri et al 2011) and insulin glycation (Kasabri et al 2010) 21 mM per cm). The results were expressed as % inhibition as calculated by the following formula-% Inhibition=[(A 0 −A 1 )/ A 0 ]×100, where A 0 is the absorbance of protein carbonyls of the positive control and A 1 is the absorbance of glycated albumin samples coincubated with plant extracts.…”

The medicinal properties and phytochemistry of plants of the genus Terminalia (Combretaceae)

“…Antidiabetic activities have also been reported for several Asian Terminalia species including T. arjuna (Ragavan and Kumari 2006), T. bellerica (Kannan et al 2012;Kasabri et al 2010), T. catappa (Ahmed et al 2005;Nagappa et al 2003), T. chebula (Kumar et al 2006;Rao et al 2006), T. pallida and T. paniculata (Ramachandran et al 2012). T. chebula ethanolic extracts were found to decrease the levels of blood glucose and glycosylated haemoglobin in a streptozocin-induced diabetic rat model (Kumar et al 2006).…”

Uncovering potential of Indonesian medicinal plants on glucose uptake enhancement and lipid suppression in 3T3-L1 adipocytes

“…Roxb. (Kasabri et al, 2010), and P. anisum L. (Rajeshwari et al, 2011) through various mechanisms of actions . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 3.2.…”