Streptococcus panodentis sp. nov. from the oral cavities of chimpanzees

Abstract

Three strains TKU9, TKU49 and TKU50 T , were isolated from the oral cavities of chimpanzees (Pan troglodytes). The isolates were all gram-positive, facultative anaerobic cocci that lacked catalase activity. Analysis of partial 16S rRNA gene sequences showed that the most closely related species was Streptococcus infantis (96.7%). The next most closely related species to the isolates were S. rubneri, S. mitis, S. peroris and S. australis (96.6 to 96.4%). Based on the rpoB and gyrB gene sequences, TKU50T was clustered with other member of the mitis group. Enzyme activity and sugar fermentation patterns differentiated this novel bacterium from other members of the mitis group streptococci. The DNA G þ C content of strain TKU50T was 46.7 mol%, which is the highest reported value for members of the mitis group (40-46 mol%). On the basis of the phenotypic characterization, partial 16S rRNA gene and sequences data for two housekeeping gene (gyrB and rpoB), we propose a novel taxa, S. panodentis for TKU 50 T (type strain ¼ CM 30579 T ¼ DSM 29921 T ), for these newly described isolates.

Noncontiguous finished genome sequence and description of Streptococcus timonensis sp. nov. isolated from the human stomach

“…The rpoB gene sequence of strain Marseille-P2915 T was obtained using the 1730_F and 3700_R primers as previously described [8], [9], [10], [11], [12], [13], [14], [15], [16], [17], [18], [19]. Then a BLAST search was performed against the NCBI database using default parameters.…”

“…The use of rpoB was initially proposed in our laboratory [8]. Since then, it has been validated by its use in the classification of different new streptococcal species [9], [10], [11].…”

Streptococcus chenjunshii sp. nov. isolated from feces of Tibetan antelopes

“…Streptococcus has played an important role in clinical infectious diseases, as some Streptococci are associated with endometritis, respiratory infections, endocarditis, meningitis, arthritis and mastitis [2,3]. Animals, especially wild ones, act as crucial hosts of Streptococcus [4][5][6][7][8][9][10][11]. Tibetan antelope (Pantholops hodgsonii) of the family Bovidae can tolerate an environment of low oxygen conditions, high attitude and high ambient ultraviolet radiation, typical of the Qinghai-Tibet Plateau.…”

Streptococcus oricebi sp. nov., isolated from the oral cavity of tufted capuchin

“…Novel streptococci from the oral cavity of various animals have previously been reported (Takada & Hirasawa, 2007, 2008Takada et al, 2010Takada et al, , 2013Shinozaki-Kuwahara et al, 2011Okamoto et al, 2013;Saito et al, 2014). Some oral streptococci are known to form characteristic colonies on Mitis Salivarius (MS) agar, demonstrating the synthesis of exopolysaccharides from sucrose (Hamada et al, 1979;Coykendall & Gustafson, 1985;Kilian et al, 1989).…”