<i>Stenotrophomonas maltophilia</i> Interaction with Human Epithelial Respiratory Cells <i>In Vitro</i>

Vidipó, Luciana de Abreu; Marques, Elizabeth de Andrade; Puchelle, Édith; Plotkowski, Maria-Cristina

doi:10.1111/j.1348-0421.2001.tb01287.x

Cited by 36 publications

(30 citation statements)

References 19 publications

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“…Transmission electron microscopy indicates that both cystic fibrosis (CF) and non-cystic fibrosis (NCF) S. maltophilia isolates adhere to and are able to invade transformed human bronchial epithelial 16 HBE14oϪ cells (Fig. 5) (73). No significant difference between the adherences of CF and NCF isolates to host bronchial cells was observed.…”

Section: Adherence To and Invasion Of Host Cellsmentioning

confidence: 95%

“…maltophilia can form biofilms on lung cells (73,83,268). Confocal microscopy of biofilms formed by CF isolate S. maltophilia OBGTC9 on CF sputum-derived bronchial epithelial IB3-1 cell monolayers revealed that S. maltophilia formed microcolonies embedded in a matrix (268).…”

Section: Molecular Mechanisms Involved In Pathogenesis Biofilmsmentioning

confidence: 99%

“…maltophilia can adhere to and form biofilms on human bronchial epithelial cells and is able to invade them (73,90,268). Transmission electron microscopy indicates that both cystic fibrosis (CF) and non-cystic fibrosis (NCF) S. maltophilia isolates adhere to and are able to invade transformed human bronchial epithelial 16 HBE14oϪ cells (Fig.…”

Section: Adherence To and Invasion Of Host Cellsmentioning

confidence: 99%

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Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

2012

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SUMMARY Stenotrophomonas maltophilia is an emerging multidrug-resistant global opportunistic pathogen. The increasing incidence of nosocomial and community-acquired S. maltophilia infections is of particular concern for immunocompromised individuals, as this bacterial pathogen is associated with a significant fatality/case ratio. S. maltophilia is an environmental bacterium found in aqueous habitats, including plant rhizospheres, animals, foods, and water sources. Infections of S. maltophilia can occur in a range of organs and tissues; the organism is commonly found in respiratory tract infections. This review summarizes the current literature and presents S. maltophilia as an organism with various molecular mechanisms used for colonization and infection. S. maltophilia can be recovered from polymicrobial infections, most notably from the respiratory tract of cystic fibrosis patients, as a cocolonizer with Pseudomonas aeruginosa . Recent evidence of cell-cell communication between these pathogens has implications for the development of novel pharmacological therapies. Animal models of S. maltophilia infection have provided useful information about the type of host immune response induced by this opportunistic pathogen. Current and emerging treatments for patients infected with S. maltophilia are discussed.

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Section: Adherence To and Invasion Of Host Cellsmentioning

confidence: 95%

Section: Molecular Mechanisms Involved In Pathogenesis Biofilmsmentioning

confidence: 99%

Section: Adherence To and Invasion Of Host Cellsmentioning

confidence: 99%

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Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

2012

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“…Invasion of epithelial respiratory cells was recently reported for S maltophilia CF clinical isolates (8). Uptake of our S maltophilia strains by A549 cells was assessed carrying out exclusion experiments with a combination of antibiotics (amikacin and ceftazidime) that we proved to be effective in selectively eliminating extra-cellular bacteria.…”

Section: Molecular Detection Ofvirulence Related Genesmentioning

confidence: 99%

“…S. maltophilia strains of environmental and clinical origin have been shown to be able to adhere to and to invade cultured epithelial respiratory cells, as well as to adhere to different abiotic surfaces with production of biofilm (5,8).…”

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confidence: 99%

Molecular Characterization of Virulence Determinants of Stenotrophomonas Maltophilia Strains Isolated from Patients Affected by Cystic Fibrosis

Bonaventura

Prosseda

Chierico

et al. 2007

Int J Immunopathol Pharmacol

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Stenotrophomonas maltophilia is an emerging nosocomial bacterial pathogen which is currently isolated with increasing frequency from the airways of cystic fibrosis (CF) patients. In this study 13 S. maltophilia strains (11 isolated from the airways of independent CF patients, and two non-CF respiratory reference strains) have been characterized for the expression of several virulence-associated factors. In particular, the ability to form biofilm on abiotic surfaces has been determined and correlated with different features, such as motility, adherence and the ability to invade A549 respiratory epithelial cells. Moreover, the presence of a flagellum-associated gene as well as that of the StmPr1 gene, which encodes an extra-cellular protease, have been determined by Southern blot hybridization. Our data indicate that the different degree of biofilm formation exhibited by the 11 CF isolates does not correlate with motility, ability to adhere to and invade A549 cells, or with the presence of flagella. On the other hand, among the CF isolates the StmPr1 gene was found only in two strains, both able to establish chronic lung infections in CF patients. Moreover, only four of the strains analyzed show a temperature-independent antibiotic-resistance profile, suggesting either a different origin of these strains or an intervening adaptation to host tissues.

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Effect of environmental factors on biofilm formation by clinicalStenotrophomonas maltophilia isolates

Bonaventura

Stepanović²,

Picciani

et al. 2007

Folia Microbiol

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The influence of environmental factors (temperature, aerobiosis-anaerobiosis, static-dynamic conditions, pH) was determined on biofilm formation by 51 S. maltophilia clinical isolates. The strains produced more biofilm at 32 degrees C than at 37 or 18 degrees C. Aerobic and 6% CO2 atmosphere yielded comparable biofilm amounts, higher than under anaerobic conditions. Biofilm production was not affected by static vs. agitated culture conditions. Biofilm production at pH 7.5 and 8.5 was comparable but significantly higher than at pH 5.5. The capacity of individual strains to form biofilm and thus contribute to the severity of some diseases is influenced by host traits and environmental conditions at the site of infection, and play an important role in the pathogenesis of biomaterial-related disease caused by S. maltophilia.

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Stenotrophomonas maltophilia Interaction with Human Epithelial Respiratory Cells In Vitro

Cited by 36 publications

References 19 publications

Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

Molecular Characterization of Virulence Determinants of Stenotrophomonas Maltophilia Strains Isolated from Patients Affected by Cystic Fibrosis

Effect of environmental factors on biofilm formation by clinicalStenotrophomonas maltophilia isolates

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