Staphylococcus aureusisolates belonging to different multilocus sequence types present specific virulence gene profiles

Abstract

To characterize 73 Staphylococcus aureus isolates from infections in an orthopedic hospital in Rio de Janeiro, we investigated the SCCmec types, the clonality by pulsed field gel electrophoresis and multilocus sequence typing, and the presence of virulence genes. Twenty‐eight (38.3%) methicillin‐resistant (16 SCCmec type IV and 12 type III) isolates were detected. Most (83.5%) of the isolates were included in five lineages: sequence type (ST) 239 (SCCmecIII), 1, 5, 30, and 1462 (SCCmecIV and/or methicillin‐susceptible isolates). Virulence genes fnbB, bbp, and pvl were related to STs 239, 30, and 30/1462, respectively. Isolates from STs 1, 5, and 30 presented specific virulence profiles, irrespective of methicillin resistance.

Molecular characterization of methicillin-resistantStaphylococcus aureusresistant to tigecycline and daptomycin isolated in a hospital in Brazil

“…Although this study involves only one Brazilian hospital, the results reflect the decline in the prevalence of the BEC lineage and its replacement by other international clones, notably CC5 [3]. However, unlike other studies, we have identified a significant aggravating factor of clonal expansion of CC5 strains resistant to tigecycline but markedly less so to daptomycin.…”

Molecular characterization of Staphylococcus aureus isolates carrying the Panton-Valentine leukocidin genes from Rio de Janeiro hospitals

“…This lineage carrier of PVL genes had already been reported in Argentina (Egea et al, 2014;Fernandez et al, 2012) and in Brazil (Cavalcante et al, 2015;Caboclo et al, 2013;Schuenck et al, 2012). Moreover, in the present study, a VISA isolate belonging to USA1100/ST30 was identified, indicating the relevance of this lineage among the PVL-positive MRSA isolates.…”

“…The bbp virulence gene, whose presence could be associated to osteomyelitis and arthritis (Tristan et al, 2003) had been related to the ST30 S. aureus lineage found in an orthopedic hospital (Schuenck et al, 2012). Here, we also found a correlation between the ST30 and the bbp gene, suggesting that this gene could be a possible marker of this lineage.…”

“…Furthermore, analysis of a collection of bacterial isolates having a common characteristic, such as PVL genes, can help to understand the relationship between them. However, in general, available studies demonstrate only the occurrence of these isolates (Nichol et al, 2013;Cavalcante et al, 2015;Caboclo et al, 2013;Egea et al, 2014;Fernandez et al, 2012;Schuenck et al, 2012). In the present study, we analyzed a collection of 50 PVL-positive S. aureus isolates obtained in a 6-year period in Rio de Janeiro hospitals as their phenotypic and molecular characteristics.…”

Tendência de resistência entre isolados clínicos de Staphylococcus aureus em um hospital universitário do norte do Paraná de 2002 a 2011

“…Em adição, tem sido observado em todo mundo, assim como no Brasil, a emergência de clones de MRSA que se apresentam sensíveis a vários antimicrobianos não betalactâmicos, em especial sulfametoxazoltrimetoprim (MATOS et al, 2014;MIRANDA et al, 2007;SCHUENCK et al, 2012;TRINDADE et al, 2005). Estas observações concordam com as realizadas no presente estudo, uma vez que a sensibilidade a sulfametoxazol-trimetoprim e gentamicina diminuíram 32 e 41%, de 2002 a 2011, respectivamente.…”