<i>SPO21</i>Is Required for Meiosis-specific Modification of the Spindle Pole Body in Yeast

Bajgier, Bethany K.; Malzone, Maria; Nickas, Mark E.; Neiman, Aaron M.

doi:10.1091/mbc.12.6.1611

Cited by 54 publications

(100 citation statements)

References 48 publications

(60 reference statements)

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“…At meiosis II, Spc72 disappears (presumably by proteolysis) and several sporulation-specific proteins are recruited to form a greatly expanded cytoplasmic face termed the meiosis II outer plaque (MOP) (Moens and Rapport 1971;Knop and Strasser 2000) (Figure 2). The major MOP proteins are Spo21/Mpc70, Mpc54, and Spo74 (Knop and Strasser 2000;Bajgier et al 2001;Nickas et al 2003). The constitutive SPB proteins Cnm67 and Nud1 are also present in the MOP, as is Ady4, a minor component important for MOP complex stability (Knop and Strasser 2000;Nickas et al 2003;Mathieson et al 2010a).…”

Section: Key Events In the Phases Of Sporulationmentioning

confidence: 99%

“…In mutants lacking Mpc54, Spo21, or Spo74, an organized MOP does not assemble on the SPB and hence no prospore membranes are formed (Knop and Strasser 2000;Bajgier et al 2001;Nickas et al 2003). That the MOP specifies where prospore membranes form is shown by experiments in cnm67D mutant cells (Bajgier et al 2001), which lose the link between the MOP and the SPB. As a result, MOP complexes assemble at ectopic sites in the cytoplasm and generate prospore membranes that fail to capture daughter nuclei.…”

Section: Key Events In the Phases Of Sporulationmentioning

confidence: 99%

“…It is not known how the reduction in spore number is achieved. But it is noteworthy that strains heterozygous for deletion of any of the major MOP component genes (MPC54, SPO21, or SPO74) display increased nonsister dyad formation in normal sporulation conditions, suggesting that reduced expression of one or all of these genes could underlie the response (Bajgier et al 2001;Wesp et al 2001;Nickas et al 2003). Indeed, sporulation in limited acetate leads to reductions in the levels of the MOP proteins plus the leading edge proteins Ady3 and Ssp1 (Taxis et al 2005).…”

Section: Transition To Meiotic Division: Control Of Ndt80mentioning

confidence: 99%

“…Surprisingly, once begun there is no apparent feedback control between meiotic events and prospore membrane growth. For example, mutants defective in membrane assembly nonetheless progress through the meiotic divisions with normal kinetics (Nag et al 1997;Bajgier et al 2001). Similarly, the arrest or delay of meiotic events does not induce a corresponding change in membrane growth (Schild and Byers 1980).…”

Section: Transition To Meiotic Division: Control Of Ndt80mentioning

confidence: 99%

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Sporulation in the Budding Yeast Saccharomyces cerevisiae

2011

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In response to nitrogen starvation in the presence of a poor carbon source, diploid cells of the yeast Saccharomyces cerevisiae undergo meiosis and package the haploid nuclei produced in meiosis into spores. The formation of spores requires an unusual cell division event in which daughter cells are formed within the cytoplasm of the mother cell. This process involves the de novo generation of two different cellular structures: novel membrane compartments within the cell cytoplasm that give rise to the spore plasma membrane and an extensive spore wall that protects the spore from environmental insults. This article summarizes what is known about the molecular mechanisms controlling spore assembly with particular attention to how constitutive cellular functions are modified to create novel behaviors during this developmental process. Key regulatory points on the sporulation pathway are also discussed as well as the possible role of sporulation in the natural ecology of S. cerevisiae. TABLE OF CONTENTS Abstract 737Introduction 738

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Section: Key Events In the Phases Of Sporulationmentioning

confidence: 99%

Section: Key Events In the Phases Of Sporulationmentioning

confidence: 99%

Section: Transition To Meiotic Division: Control Of Ndt80mentioning

confidence: 99%

Section: Transition To Meiotic Division: Control Of Ndt80mentioning

confidence: 99%

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Sporulation in the Budding Yeast Saccharomyces cerevisiae

2011

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“…As a consequence of this process, four haploid gametes (spores) are formed in the cytoplasm of the mother cell (ascus) (reviewed by Neiman 2005). At the onset of meiosis II, the spindle pole body (SPB) is modified by the incorporation of several meiosis-specific proteins, which together make up the meiosis II outer plaque (Knop and Strasser 2000;Bajgier et al 2001;Nickas et al 2003). This change in composition converts the function of the cytoplasmic face of the SPB from microtubule nucleation to membrane nucleation.…”

mentioning

confidence: 99%

Cdc15 Is Required for Spore Morphogenesis Independently of Cdc14 in Saccharomyces cerevisiae

et al. 2007

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In Saccharomyces cerevisiae exit from mitosis requires the Cdc14 phosphatase to reverse CDK-mediated phosphorylation. Cdc14 is released from the nucleolus by the Cdc14 early anaphase release (FEAR) and mitotic exit network (MEN) pathways. In meiosis, the FEAR pathway is essential for exit from anaphase I. The MEN component Cdc15 is required for the formation of mature spores. To analyze the role of Cdc15 during sporulation, a conditional mutant in which CDC15 expression was controlled by the CLB2 promoter was used. Cdc15-depleted cells proceeded normally through the meiotic divisions but were unable to properly disassemble meiosis II spindles. The morphology of the prospore membrane was aberrant and failed to capture the nuclear lobes. Cdc15 was not required for Cdc14 release from the nucleoli, but it was essential to maintain Cdc14 released and for its nucleo-cytoplasmic transport. However, cells carrying a CDC14 allele with defects in nuclear export (Cdc14-DNES) were able to disassemble the spindle and to complete spore formation, suggesting that the Cdc14 nuclear export defect was not the cause of the phenotypes observed in cdc15 mutants. MEIOSIS is a specialized cell division that produces the haploid cells needed for sexual reproduction, and the completion of meiosis is normally coupled to differentiation programs that produce gametes. In Saccharomyces cerevisiae, sporulation is initiated when diploid cells are grown in the absence of nitrogen and the presence of a nonfermentable carbon source such as acetate (Esposito and Klapholtz 1981). When triggered to enter the sporulation program, cells exit the mitotic cycle from the G1 phase. This is followed by one round of S phase and two meiotic divisions that generate four haploid nuclei, which are packaged into individual spores. As a consequence of this process, four haploid gametes (spores) are formed in the cytoplasm of the mother cell (ascus) (reviewed by Neiman 2005). At the onset of meiosis II, the spindle pole body (SPB) is modified by the incorporation of several meiosis-specific proteins, which together make up the meiosis II outer plaque (Knop and Strasser 2000;Bajgier et al. 2001;Nickas et al. 2003). This change in composition converts the function of the cytoplasmic face of the SPB from microtubule nucleation to membrane nucleation. The process of spore construction requires the de novo assembly of two cellular structures, the prospore membrane (PSM) that is generated around the daughter nuclei to create prospores and a protective spore wall that surrounds the prospores. Once the meiosis II outer plaque of the SPB has been assembled, it becomes a site for the docking and coalescence of secretory vesicles to form flattened double-membrane sheets termed PSMs (Moens 1971;Neiman 1998). These nascent PSMs expand during meiosis II, and at the time of nuclear division each PSM completely engulfs the nuclear lobe to which it is anchored via the SPB. At the completion of meiosis II, each PSM fuses with itself so that each daughter nucleus (and assoc...

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Spindle Pole Body ( SPB )

2004

Encyclopedic Dictionary of Genetics, Genomics and Proteomics

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SPO21Is Required for Meiosis-specific Modification of the Spindle Pole Body in Yeast

Cited by 54 publications

References 48 publications

Sporulation in the Budding Yeast Saccharomyces cerevisiae

Sporulation in the Budding Yeast Saccharomyces cerevisiae

Cdc15 Is Required for Spore Morphogenesis Independently of Cdc14 in Saccharomyces cerevisiae

Spindle Pole Body ( SPB )

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