<i>SlPMEI</i>, a pollen-specific gene in tomato

Kim, Woong Bom; Lim, Chan Ju; Jang, Hyun A; Yi, So Young; Oh, Sang‐Keun; Lee, Ha Yeon; Kim, Hyun A; Park, Youn‐Il; Kwon, Suk‐Yoon

doi:10.4141/cjps2013-084

Cited by 10 publications

(9 citation statements)

References 53 publications

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“…SlCRK1 , a cysteine-rich receptor-like kinase, plays a critical role in pathogen protection and programmed cell death 45 . Pectin methylesterase inhibitor ( SlPMEI ) acts as a key regulator of pectin methylesterase (PME) 46 . LePRK3 , a pollen-specific receptor kinase gene, may take part in perceiving extracellular cues during pollen tube growth 47 .…”

Section: Resultsmentioning

confidence: 99%

“…All of these genes showed a trend of downregulation in the transgenic lines, suggesting that SlOFP20 may control the mRNA accumulation of these pollen-specific genes to impact pollen development. In addition, previous studies revealed that many pollen-specific cis-acting elements, including the pollen-specific activation-related elements POLLEN1LELAT52 and the late pollen gene g10-related elements, were enriched in the promoter regions of two pollen-specific genes, SlCRK1 45 and SlPMEI 46 . Promoter-GUS chimeric expression experiments have been used to confirm that the promoters of SlCRK1 45 and SlPMEI 46 exhibit strong pollen-specific activity in the transgenic Arabidopsis and tomato plants.…”

Section: Discussionmentioning

confidence: 99%

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Overexpression of SlOFP20 affects floral organ and pollen development

Zhou

et al. 2019

Hortic Res

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The OVATE gene was initially identified in tomato and serves as a key regulator of fruit shape. There are 31 OFP members in the tomato genome. However, their roles in tomato growth and reproductive development are largely unknown. Here, we cloned the OFP transcription factor SlOFP20. Tomato plants overexpressing SlOFP20 displayed several phenotypic defects, including an altered floral architecture and fruit shape and reduced male fertility. SlOFP20 overexpression altered the expression levels of some brassinosteroid (BR)-associated genes, implying that SlOFP20 may play a negative role in the BR response, similar to its ortholog OsOFP19 in rice. Moreover, the transcript accumulation of gibberellin (GA)-related genes was significantly affected in the transgenic lines. SlOFP20 may play an important role in the crosstalk between BR and GA. The pollen germination assay suggested that the pollen germination rate of SlOFP20-OE plants was distinctly lower than that of WT plants. In addition, the tomato pollen-associated genes SlCRK1, SlPMEI, LePRK3, SlPRALF, and LAT52 were all suppressed in the transgenic lines. Our data imply that SlOFP20 may affect floral organ and pollen development by modulating BR and GA signaling in tomato.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Overexpression of SlOFP20 affects floral organ and pollen development

Zhou

et al. 2019

Hortic Res

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“…Further, we assessed the expression of tomato pollen-specific genes involved in the pollen development in WT and SlGLO1 -silenced lines. SlCRK1 is one of the cysteine-rich receptor-like kinases, is important in pathogen defense and programmed cell death 33 ; Pectin methylesterase inhibitor (SlPMEI) is key regulators of pectin methylesterase (PME) 34 ; Pollen-specific receptor kinases gene LePRK3 is likely to be involved in perceiving extracellular cues during pollen tube growth 35 ; Exogenous rapid alkalinization factor (SlPRALF) acts as a negative regulator of pollen tube elongation within a specific developmental window 36 and LAT52 may play a role during germination or early tube growth 37 . The quantitative PCR results showed that these genes were all down-regulated by more than 90% in SlGLO1 -silenced lines ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies about two pollen-specific genes including SlCRK1 and SlPMEI showed that their promoters region possess lots of pollen-specific cis-acting elements, such as, the pollen-specific activation-related elements POLLEN1LELAT52 (Lat52, AGAAA) and late pollen gene g10-related elements (G10, GTGA) 33 34 . Kim et al 33 34 also confirmed promoters of SlCRK1 and SlPMEI have strong pollen-specific activity in the homozygous transgenic plants of tomato. Similarly, we analyzed putative cis-acting elements within the SlGLO1 promoter.…”

Section: Discussionmentioning

confidence: 99%

The tomato floral homeotic protein FBP1-like gene, SlGLO1, plays key roles in petal and stamen development

Guo

Yin

et al. 2016

Sci Rep

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MADS-box transcription factors play important role in plant growth and development, especially floral organ identities. In our study, a MADS-box gene SlGLO1- tomato floral homeotic protein FBP1-like gene was isolated. Its tissue-specific expression profile analysis showed that SlGLO1 was highly expressed in petals and stamens. RNAi (RNA interference) repression of SlGLO1 resulted in floral organ abnormal phenotypes, including green petals with shorter size, and aberrant carpelloid stamens. SlGLO1-silenced lines are male sterile. Total chlorophyll content was increased and chlorophyll biosynthetic genes were significantly up-regulated in SlGLO1-silenced petals and stamens. Furthermore, B-class genes expression analysis indicated that the repressed function of SlGLO1 led to the enhanced expression of TAP3 and the down-regulation of TPI in the petals and stamens, while the expression of TM6 was reduced in petals and increased in stamens and carpels of SlGLO1-RNAi plants. Additionally, pollen grains of transgenic lines were aberrant and failed to germinate and tomato pollen-specific genes were down-regulated by more than 90% in SlGLO1-silenced lines. These results suggest that SlGLO1 plays important role in regulating plant floral organ and pollen development in tomato.

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“…Arabidopsis has been widely used to effectively characterize the cis -elements of the anther/pollen-specific promoters for homologous and heterologous crop plant species ( Swapna et al, 2011 ; Kim et al, 2014 ; Gao et al, 2016 ). In our study, the activities of pBnaC.SP6 deletions were analyzed in Arabidopsis .…”

Section: Discussionmentioning

confidence: 99%

BnaA.bZIP1 Negatively Regulates a Novel Small Peptide Gene, BnaC.SP6, Involved in Pollen Activity

Wang

et al. 2017

Front. Plant Sci.

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Small peptides secreted to the extracellular matrix control many aspects of the plant’s physiological activities which were identified in Arabidopsis thaliana, called ATSPs. Here, we isolated and characterized the small peptide gene Bna.SP6 from Brassica napus. The BnaC.SP6 promoter was cloned and identified. Promoter deletion analysis suggested that the -447 to -375 and -210 to -135 regions are crucial for the silique septum and pollen expression of BnaC.SP6, respectively. Furthermore, the minimal promoter region of p158 (-210 to -52) was sufficient for driving gene expression specifically in pollen and highly conserved in Brassica species. In addition, BnaA.bZIP1 was predominantly expressed in anthers where BnaC.SP6 was also expressed, and was localized to the nuclei. BnaA.bZIP1 possessed transcriptional activation activity in yeast and protoplast system. It could specifically bind to the C-box in p158 in vitro, and negatively regulate p158 activity in vivo. BnaA.bZIP1 functions as a transcriptional repressor of BnaC.SP6 in pollen activity. These results provide novel insight into the transcriptional regulation of BnaC.SP6 in pollen activity and the pollen/anther-specific promoter regions of BnaC.SP6 may have their potential agricultural application for new male sterility line generation.

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SlPMEI, a pollen-specific gene in tomato

Cited by 10 publications

References 53 publications

Overexpression of SlOFP20 affects floral organ and pollen development

Overexpression of SlOFP20 affects floral organ and pollen development

The tomato floral homeotic protein FBP1-like gene, SlGLO1, plays key roles in petal and stamen development

BnaA.bZIP1 Negatively Regulates a Novel Small Peptide Gene, BnaC.SP6, Involved in Pollen Activity

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