<i>Shigella flexneri</i>Bacteremia in Two Immune‐Competent Adult Travelers

Grondin, Cynthia; Imbert, Patrick; Ficko, C.; Mérens, Audrey; Dutasta, F.; Bigaillon, C.; Rapp, C.

doi:10.1111/j.1708-8305.2012.00627.x

Cited by 8 publications

(6 citation statements)

References 11 publications

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“…Shigellosis is diagnosed through laboratory testing of stool samples for the presence of Shigella cells. Shigellosis may lead to bacteremia . In this article, we show that a modified immunosensing system that provides voltage-controlled intrinsic signal amplification is able to detect inactivated Shigella directly in stool and blood at the single-digit CFU level.…”

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confidence: 93%

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Ultrasensitive Detection of Shigella Species in Blood and Stool

et al. 2016

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A modified immunosensing system with voltage-controlled signal amplification was used to detect Shigella in stool and blood matrixes at the single-digit CFU level. Inactivated Shigella was spiked in these matrixes and detected directly. The detection was completed in 78 min. Detection limits of 21 CFU/mL and 18 CFU/mL were achieved in stool and blood, respectively, corresponding to 2-7 CFUs immobilized on the detecting electrode. The outcome of the detection of extremely low bacterium concentration, i.e., below 100 CFU/mL, blood samples show a random nature. An analysis of the detection probabilities indicates the correlation between the sample volume and the success of detection and suggests that sample volume is critical for ultrasensitive detection of bacteria. The calculated detection limit is qualitatively in agreement with the empirically determined detection limit. The demonstrated ultrasensitive detection of Shigella on the single-digit CFU level suggests the feasibility of the direct detection of the bacterium in the samples without performing a culture.

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confidence: 93%

“…Shigellosis may lead to bacteremia. 5 In this article, we show that a modified immunosensing system that provides voltage-controlled intrinsic signal amplification is able to detect inactivated Shigella directly in stool and blood at the single-digit CFU level. The detection in stool samples is characterized by a wide linear range from 50 to 3 × 10 4 CFU/mL.…”

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confidence: 94%

Ultrasensitive Detection of Shigella Species in Blood and Stool

et al. 2016

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“…Shigella is an infectious food-borne pathogen that can cause a severe diarrhea illness called shigellosis (1)(2)(3). It is responsible for approximately 165 million cases of diarrhea annually, of which 163 million are in developing countries and 1.5 million are in industrialized ones (4,5).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Shigella species and Antimicrobial Resistance Patterns of Isolated Strains from Infected Pediatrics in Tehran

Mardaneh¹,

Poor²,

Afrugh³

2013

Int J Enteric Pathog

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Background:Shigella is an infectious food-borne pathogen that can cause a severe diarrhea illness called shigellosis. The increasing levels of antimicrobial resistance of Shigella isolates have complicated the treatment of shigellosis. The antimicrobial resistance patterns of Shigella species vary according to geographic region and in the same place over time, leading to a therapeutic problem. Objectives: This study attempts to determine the prevalence of shigellosis and the antimicrobial susceptibility pattern of isolated strains. Materials and Methods:This cross-sectional study, with a 12-month duration, between August 2010 and August 2011 was carried out on 9131 infants with acute diarrheal disease. Stool samples were inoculated on selective and differential media. The Shigella isolates were identified and confirmed by biochemical laboratory tests. Subsequently, serotyping was performed with group specific antisera. Drug sensitivity test was carried out according to CLSI (2010) recommendations by using the standard disc diffusion method. Results: Shigella spp. were isolated from 90 (1%) of the 9131 stool samples collected from children with acute diarrhea. Among Shigella isolates, the most common subgroup was S. sonnei 63 (70%). Our results showed that imipenem, ciprofloxacin, ceftazidime and ceftizoxime are most effective antimicrobial agents against Shigellasp. The most frequent resistance observed, was towards co-trimoxazole (92.2%), ampicillin (65.6%) and tetracycline (65.6%) Conclusion: Since antibiotic resistant profile of Shigella varies geographically and also over time within a single country, regular, continuous surveillance is necessary. The results of accurate surveillance should be used to guide policies for antibiotic prescription.

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“…La distribución según especies fue la siguiente: S. flexneri 70,9%, S. sonnei 23,1%, Shigella spp. 5,8% y Shigella boydii 0,1%; coincidiendo con los datos encontrados en otros centros de nuestro país [8][9][10] .…”

Section: Discussionunclassified

“…Con respecto a la susceptibilidad antimicrobiana, en un estudio realizado en las provincias de Chaco y Corrientes, entre 1998 y 2002, a partir de 132 aislados de Shigella (78% S. flexneri y 22% S. sonnei), se observó mayor resistencia en S. flexneri (p < 0,001). El patrón de resistencia para la misma fue: ampicilina 80,6%; cotrimoxazol 59,2%; ampicilina-sulbactam 45,6%; cefalotina 16,5%; sin resistencia a ciprofloxacina 10 .…”

Section: Enunclassified