Ultrasensitive Detection of Shigella Species in Blood and Stool

Abstract

A modified immunosensing system with voltage-controlled signal amplification was used to detect Shigella in stool and blood matrixes at the single-digit CFU level. Inactivated Shigella was spiked in these matrixes and detected directly. The detection was completed in 78 min. Detection limits of 21 CFU/mL and 18 CFU/mL were achieved in stool and blood, respectively, corresponding to 2-7 CFUs immobilized on the detecting electrode. The outcome of the detection of extremely low bacterium concentration, i.e., below 100 CFU/mL, blood samples show a random nature. An analysis of the detection probabilities indicates the correlation between the sample volume and the success of detection and suggests that sample volume is critical for ultrasensitive detection of bacteria. The calculated detection limit is qualitatively in agreement with the empirically determined detection limit. The demonstrated ultrasensitive detection of Shigella on the single-digit CFU level suggests the feasibility of the direct detection of the bacterium in the samples without performing a culture.

Culture-free bacterial detection and identification from blood with rapid, phenotypic, antibiotic susceptibility testing

“…S1 and S2 ). The method employs an immuno-assay platform 16 , 17 for the direct detection of bacteria in samples without culture enrichment. Fig.…”

Nonlytic Recombinant Phage Tail Fiber Protein for Specific Recognition of Pseudomonas aeruginosa

“…The typical molecular recognition elements for pathogenic bacteria include an antimicrobial peptide, , antibiotic, quaternary ammonium, and carbohydrate. , Although these molecular recognition elements show outstanding merits such as a low cost and high stability, their application is greatly limited by the poor specificity for recognizing given bacteria. Alternatively, an aptamer , and antibody , show greatly improved specificity for bacterial recognition, whereas they sometimes suffer from poor repeatability and batch variability. − Furthermore, the screening of the aptamer and antibody with a high specificity and strong binding activity is always time-consuming and labor-intensive.…”

Chemical Monitoring in Clinical Settings: Recent Developments toward Real-Time Chemical Monitoring of Patients

“…186 Identification of bacterial infections linked to gastric disorders is possible by microfluidic nucleic acid-based analysis and by an ultrasensitive immunosensing system with voltage-controlled signal amplification. 187,188 ■ FUTURE PERSPECTIVES Since our first review in this area, 189 we present in this Review a dramatic increase in the progression of analytical technologies along the clinical translation time axis in Figure 1B. Interestingly, this progress is much greater than would have been predicted in 2013 on the basis of our future technology directions horizon scanning.…”

“…186 Identification of bacterial infections linked to gastric disorders is possible by microfluidic nucleic acid-based analysis and by ultrasensitive immunosensing system with voltage-controlled signal amplification. 187,188…”

“…Analysis of stool samples helps to understand the gut microbiome by untargeted mass spectrometry . Identification of bacterial infections linked to gastric disorders is possible by microfluidic nucleic acid-based analysis and by an ultrasensitive immunosensing system with voltage-controlled signal amplification. , …”