SEUSSandAINTEGUMENTAMediate Patterning and Ovule Initiation during Gynoecium Medial Domain Development    

Abstract

The Arabidopsis (Arabidopsis thaliana) gynoecium, the female floral reproductive structure, requires the action of genes that specify positional identities during its development to generate an organ competent for seed development and dispersal. Early in gynoecial development, patterning events divide the primordium into distinct domains that will give rise to specific tissues and organs. The medial domain of the gynoecium gives rise to the ovules, and several other structures critical for reproductive competence. Here we report a synergistic genetic interaction between seuss and aintegumenta mutants resulting in a complete loss of ovule initiation and a reduction of the structures derived from the medial domain. We show that patterning events are disrupted early in the development of the seuss aintegumenta gynoecia and we identify PHABULOSA (PHB), REVOLUTA, and CRABS CLAW (CRC) as potential downstream targets of SEUSS (SEU) and AINTEGUMENTA (ANT) regulation. Our genetic data suggest that SEU additionally functions in pathways that are partially redundant and parallel to PHB, CRC, and ANT. Thus, SEU and ANT are part of a complex and robust molecular system that coordinates patterning cues and cellular proliferation along the three positional axes of the developing gynoecium.During organ development, positional identity information must be coordinated with cellular proliferation to achieve proper organ shape and function. In the Arabidopsis (Arabidopsis thaliana) gynoecium, the female reproductive floral structure, early patterning events divide the gynoecial primordium into distinct zones that distinguish adaxial (inner) versus abaxial (outer), medial versus lateral, and apical versus basal

Role of Plant Somatic Embryogenesis Receptor Kinases (SERKs) in Cell-to-Embryo Transitional Activity: Key at Novel Assorted Structural Subunits

“…It is thus clear that ANT transcription factors are regulated by auxin gradient suggested for auxin-mediated regulation of ANT transcription factor in SE. The ANT is part of a complex and robust molecular system that coordinates patterning signals and cellular proliferation through embryo development [58]. This data indeed provide support for the structural changes in the regulatory sequence of AtSERK1 gene evolved exclusively for its spatial expression during SE, than the regulatory sequences of AtSERK2 gene.…”

“…AINTEGUMENTA is an AP2-like ethylene responsive transcription factor, a family of proteins involved in ethylene signal transduction recognizing and binding to the DNA consensus sequence 5'-CAC[AG]N[AT] TNCCNANG-3' [58]- [61]. The trans-factor ANT has been reported for its important role in the cellular fate deciding processes throughout the development of ovule and female gametophyte; and even upto embryogenesis.…”

SEUSS Integrates Gibberellin Signaling with Transcriptional Inputs from the SHR-SCR-SCL3 Module to Regulate Middle Cortex Formation in the Arabidopsis Root

“…Previously published mutants and marker lines used in this study include seu-3 and seu-4 (Pfluger and Zambryski, 2004), ga1 (Sun and Kamiya, 1994), scr-3, shr-2, and SHR::SHR-GFP (Nakajima et al, 2001), SCR::H2B-YFP (Heidstra et al, 2004), scl3, SCL3::GUS, and SCL3::SCL3-GFP , CYCD6;1::GFP-GUS (Sozzani et al, 2010), and SEU::SEU-GFP (Azhakanandam et al, 2008). Primers used for mutant genotyping are listed in Supplemental Table S1.…”

“…nA, No antibody; Ab, GFP antibody. Azhakanandam et al, 2008;Bao et al, 2010;Nole-Wilson et al, 2010;Lee et al, 2014). Interestingly, although SEU does not appear to possess a recognizable DNA-binding motif, it was shown by chromatin immunoprecipitation (ChIP) to associate with cis-regulatory elements located in the second intron of the floral homeotic gene AGAMOUS (AG; Sridhar et al, 2006) and with cisregulatory elements of miR172 genes (Grigorova et al, 2011).…”

Temporal and tissue expression of genes involved in buds of earliness cotton cultivar

“…This agrees with the results shown in Figure 2, as the expression pattern of this gene was reduced by 70% when buds were next to the anthesis and therefore when all gametic structures had been generated. In A. thaliana, functional analysis indicated that SEUSS plays other roles in flower development, specifically in the carpel margin meristems, a vital meristematic structure located at the margins of the fused carpels and that gives rise to several structures in the female reproductive system, including the eggs (Franks et al, 2002;Azhakanandam et al, 2008;Grigorova et al, 2011;Kamiuchi et al, 2014).…”