I-SceI and customized meganucleases-mediated genome editing in tomato and oilseed rape

Danilo, Benoit; Montes, Émilie; Archambeau, Héloïse; Lodé, Maryse; Rousseau‐Gueutin, Mathieu; Chèvre, Anne‐Marie; Mazier, Marianne

doi:10.1007/s11248-021-00287-2

Cited by 8 publications

(1 citation statement)

References 38 publications

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“…Meganucleases have been classified into five families on the basis of their sequence and motifs present in their structure. These include LAGLIDADG, PD-(D/E) XK, His-Cys box, GIY-YIG, and HNH [39,40]. Among these, the LAGLIDADG meganuclease (LMN) family has been extensively used for genome editing.…”

Section: Different Plant Genome Editing Techniquesmentioning

confidence: 99%

Genome Editing: A Promising Approach for Achieving Abiotic Stress Tolerance in Plants

Kaur

Sharma

Hasanuzzaman

et al. 2022

International Journal of Genomics

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The susceptibility of crop plants towards abiotic stresses is highly threatening to assure global food security as it results in almost 50% annual yield loss. To address this issue, several strategies like plant breeding and genetic engineering have been used by researchers from time to time. However, these approaches are not sufficient to ensure stress resilience due to the complexity associated with the inheritance of abiotic stress adaptive traits. Thus, researchers were prompted to develop novel techniques with high precision that can address the challenges connected to the previous strategies. Genome editing is the latest approach that is in the limelight for improving the stress tolerance of plants. It has revolutionized crop research due to its versatility and precision. The present review is an update on the different genome editing tools used for crop improvement so far and the various challenges associated with them. It also highlights the emerging potential of genome editing for developing abiotic stress-resilient crops.

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Section: Different Plant Genome Editing Techniquesmentioning

confidence: 99%